################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HphI KpnI Ksp632I MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AfaI 507 GTAC GT^AC Asp700I 417 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 253 GAGCTC G[AT]GC[AT]^C AvaI 626 CCCGGG C^[CT]CG[GA]G BbrPI 763 CACGTG CAC^GTG BglI 264 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 723 GCTGAGC GC^T[ACGT]AGC Bpu1102I 723 GCTGAGC GC^T[ACGT]AGC BsaAI 763 CACGTG [CT]AC^GT[GA] BsaI 631 GAGACC GGTCTC(1/5) BsgI 512 GTGCAG GTGCAG(16/14) BsiEI 57 CGGCCG CG[GA][CT]^CG BsiHKAI 253 GAGCTC G[AT]GC[AT]^C BsmAI 631 GAGAC GTCTC(1/5) BsmFI 468 GTCCC GGGAC(10/14) BsoBI 626 CCCGGG C^[CT]CG[GA]G BsrFI 220 GCCGGC [GA]^CCGG[CT] BssSI 527 CTCGTG CACGAG(-5/-1) BstF5I 315 GGATG GGATG(2/0) CelII 723 GCTGAGC GC^T[ACGT]AGC Cfr10I 220 GCCGGC [GA]^CCGG[CT] EaeI 57 CGGCCG [CT]^GGCC[GA] EagI 57 CGGCCG C^GGCCG EclXI 57 CGGCCG C^GGCCG Eco52I 57 CGGCCG C^GGCCG EcoNI 487 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 266 CCAAGG C^C[AT][AT]GG FokI 315 GGATG GGATG(9/13) HincII 457 GTTAAC GT[CT]^[GA]AC HindII 457 GTTAAC GT[CT]^[GA]AC HinfI 346 GACTC G^A[ACGT]TC HpaI 457 GTTAAC GTT^AAC KspI 48 CCGCGG CCGC^GG MaeII 764 ACGT A^CGT MaeIII 344 GTGAC ^GT[ACGT]AC MseI 458 TTAA T^TAA NaeI 220 GCCGGC GCC^GGC NgoMIV 220 GCCGGC G^CCGGC PflMI 543 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 346 GACTC GAGTC(4/5) PmaCI 763 CACGTG CAC^GTG PmlI 763 CACGTG CAC^GTG RsaI 507 GTAC GT^AC SacI 253 GAGCTC GAGCT^C SacII 48 CCGCGG CCGC^GG SmaI 626 CCCGGG CCC^GGG SmlI 353 CTCAAG C^T[CT][GA]AG StuI 419 AGGCCT AGG^CCT StyI 266 CCAAGG C^C[AT][AT]GG Tru9I 458 TTAA T^TAA Tsp45I 344 GTGAC ^GT[GC]AC Van91I 543 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 323 CCAAAGGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 626 CCCGGG C^CCGGG XmaI 626 CCCGGG C^CCGGG XmnI 417 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsrBI 73 CCGCTC CCGCTC(-3/-3) 169 GAGCGG BsrI 368 ACTGG ACTGG(1/-1) 732 ACTGG BstAPI 228 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 276 GCAGCGACTGC HgaI 63 GACGC GACGC(5/10) 410 GCGTC MslI 351 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 606 CACAGACATG NspI 611 ACATGC [GA]CATG^[CT] 650 ACATGC PpuMI 545 AGGTCCT [GA]G^G[AT]CC[CT] 594 AGGTCCT PstI 274 CTGCAG CTGCA^G 664 CTGCAG PvuII 145 CAGCTG CAG^CTG 232 CAGCTG SfcI 274 CTGCAG C^T[GA][CT]AG 664 CTGCAG TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^ 374 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV Sau96I ScrFI SfoI TseI