Restriction map for
pFN21AE6245
(Circular) (Six-base) MAPSORT of: pFN21AE6245.seq Check: 4996 from: 1 to: 5488
pFN21AE6245 5488 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 2699 3551 4935 5203 1632
Size: 809 258 852 1384 268 1917
Fragments arranged by size:
1917 1384 852 809 268 258
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 2360 1166
Size: 1194 4294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 2375
Size: 32 5456
AlwNI CAG_nnn'CTG
Cuts at: 2239 2287 2554 2677 2725 3231 4803 2239
Size: 48 267 123 48 506 1572 2924
Fragments arranged by size:
2924 1572 506 267 123 48 48
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 2813 1870
Size: 73 688 182 4545
Fragments arranged by size:
4545 688 182 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2200 2216 2246 2809
Size: 325 217 627 381 32 16 30 563
Cuts at: 2809 4264 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 563 381 325 217 32
30 16
BanII G_rGCy'C
Cuts at: 1391 2262 2312 2767 2807 1391
Size: 871 50 455 40 4072
Fragments arranged by size:
4072 871 455 50 40
BbeI G_GCGC'C
Cuts at: 2172 2220 2250 2172
Size: 48 30 5410
Fragments arranged by size:
5410 48 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2275 4099 136
Size: 107 122 71 631 605 603 1824 1525
Fragments arranged by size:
1824 1525 631 605 603 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2729 2905 2729
Size: 176 5312
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2380 2485 3549 4705 1790
Size: 415 175 105 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 415 175 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 3219 4177
Size: 416 53 83 399 24 966 836 958
Cuts at: 4177 442
Size: 1753
Fragments arranged by size:
1753 966 958 836 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2582 2781 4148 1239
Size: 935 408 199 1367 2579
Fragments arranged by size:
2579 1367 935 408 199
Bpu10I CC'TnA_GC
Cuts at: 2120 2120
Size: 5488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2343 3613 4478 4776 2343
Size: 1270 865 298 3055
Fragments arranged by size:
3055 1270 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 4151 882
Size: 617 1131 1521 2219
Fragments arranged by size:
2219 1521 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 2770 3279 493
Size: 711 1566 509 2702
Fragments arranged by size:
2702 1566 711 509
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2217 2247 3295
Size: 53 83 186 936 636 48 30 1048
Cuts at: 3295 3677 4312 275
Size: 382 635 1451
Fragments arranged by size:
1451 1048 936 636 635 382 186 83
53 48 30
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 2754 2784 1178
Size: 30 165 30 972 30 349 30 3882
Fragments arranged by size:
3882 972 349 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 2017 2164 2502 4691 5187 2017
Size: 147 338 2189 496 2318
Fragments arranged by size:
2318 2189 496 338 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2538 1163
Size: 573 802 4113
Fragments arranged by size:
4113 802 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 2938 3699 4727 1540
Size: 275 186 64 873 761 1028 2301
Fragments arranged by size:
2301 1028 873 761 275 186 64
BsiHKAI G_wGCw'C
Cuts at: 2262 3549 3634 4705 2262
Size: 1287 85 1071 3045
Fragments arranged by size:
3045 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2262 2312 2380 2485 2767 2807
Size: 399 415 57 50 68 105 282 40
Cuts at: 2807 3549 3634 4705 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 282 105 85
68 57 50 40
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 2176 3375 1394
Size: 429 257 96 1199 3507
Fragments arranged by size:
3507 1199 429 257 96
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2225 4132 4319 1700
Size: 110 415 1907 187 2869
Fragments arranged by size:
2869 1907 415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 2532 3548 4560 2532
Size: 1016 1012 3460
Fragments arranged by size:
3460 1016 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 2287 794
Size: 1445 48 3995
Fragments arranged by size:
3995 1445 48
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2457 2643 513
Size: 552 939 49 404 186 3358
Fragments arranged by size:
3358 939 552 404 186 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 3004 3798 3818 2141
Size: 863 794 20 3811
Fragments arranged by size:
3811 863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2935 3824
Size: 54 1425 50 275 18 232 873 889
Cuts at: 3824 5453 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 873 275 232 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2062 2935 1537
Size: 275 250 873 4090
Fragments arranged by size:
4090 873 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 3418 1353
Size: 2065 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2260 2260
Size: 5488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2441 2582 2699 2781 3551 4148
Size: 393 542 267 141 117 82 770 597
Cuts at: 4148 4935 5203 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 770 597 542 393 268 267
141 117 82
EcoNI CCTnn'n_nnAGG
Cuts at: 2496 2496
Size: 5488
EcoO109I rG'GnC_Cy
Cuts at: 2046 2551 2600 2046
Size: 505 49 4934
Fragments arranged by size:
4934 505 49
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 2127 2172 2220 2250 2510 2561 4635 1678
Size: 449 45 48 30 260 51 2074 2531
Fragments arranged by size:
2531 2074 449 260 51 48 45 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2741 2773 4129 4161 4203
Size: 32 135 32 1169 32 1356 32 42
Cuts at: 4203 4235 1373
Size: 32 2626
Fragments arranged by size:
2626 1356 1169 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2464 2832 3078 669
Size: 988 281 526 368 246 3079
Fragments arranged by size:
3079 988 526 368 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 2464 3078 2464
Size: 614 4874
KasI G'GCGC_C
Cuts at: 2168 2216 2246 2168
Size: 48 30 5410
Fragments arranged by size:
5410 48 30
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2615 3446 3805 3964 518
Size: 648 1194 255 831 359 159 2042
Fragments arranged by size:
2042 1194 831 648 359 255 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2152 2239 2904 3581 4729 4974 1606
Size: 449 97 87 665 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 665 449 245 97 87
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 5488
NarI GG'CG_CC
Cuts at: 2169 2217 2247 2169
Size: 48 30 5410
Fragments arranged by size:
5410 48 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2620 2659 2846 4391 1132
Size: 97 1391 39 187 1545 2229
Fragments arranged by size:
2229 1545 1391 187 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 1108
Size: 1446 4042
PfoI T'CCnGG_A
Cuts at: 2430 2430
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PmlI CAC'GTG
Cuts at: 2770 2770
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2375 2407 3557 3589 5070 5102 2375
Size: 32 1150 32 1481 32 2761
Fragments arranged by size:
2761 1481 1150 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2600 2551
Size: 49 5439
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2283 2673 2840 830
Size: 1453 390 167 3478
Fragments arranged by size:
3478 1453 390 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
PvuII CAG'CTG
Cuts at: 2152 2239 2152
Size: 87 5401
SacI G_AGCT'C
Cuts at: 2262 2262
Size: 5488
SacII CC_GC'GG
Cuts at: 2056 2056
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2279 2669 2836 3971 4652 4843 826
Size: 219 1234 390 167 1135 681 191 1471
Fragments arranged by size:
1471 1234 1135 681 390 219 191 167
SfoI GGC'GCC
Cuts at: 2170 2218 2248 2170
Size: 48 30 5410
Fragments arranged by size:
5410 48 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2633 2815 1872
Size: 761 182 4545
Fragments arranged by size:
4545 761 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2358 3592 4493 4755 820
Size: 197 926 415 1234 901 262 1553
Fragments arranged by size:
1553 1234 926 901 415 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2426 1517
Size: 909 4579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2271 2927 513
Size: 552 939 267 656 3074
Fragments arranged by size:
3074 939 656 552 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 3734 5446
Size: 274 80 33 51 1443 534 1223 1712
Cuts at: 5446 96
Size: 138
Fragments arranged by size:
1712 1443 1223 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2754 2786 5448 5480 2754
Size: 32 2662 32 2762
Fragments arranged by size:
2762 2662 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2335
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2631 2813 1870
Size: 761 182 4545
Fragments arranged by size:
4545 761 182
XmnI GAAnn'nnTTC
Cuts at: 2426 3617 2426
Size: 1191 4297
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI