(Linear) (Six-base) MAPSORT of: KIEE6245.seq Check: 4148 from: 1 to: 783 KIEE6245.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 694 783 Size: 436 258 89 Fragments arranged by size: 436 258 89 AleI CACnn'nnGTG Cuts at: 0 355 783 Size: 355 428 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 370 402 783 Size: 370 32 381 Fragments arranged by size: 381 370 32 AlwNI CAG_nnn'CTG Cuts at: 0 234 282 549 672 720 783 Size: 234 48 267 123 48 63 Fragments arranged by size: 267 234 123 63 48 48 AvaI C'yCGr_G Cuts at: 0 626 783 Size: 626 157 BanI G'GyrC_C Cuts at: 0 163 195 211 241 783 Size: 163 32 16 30 542 Fragments arranged by size: 542 163 32 30 16 BanII G_rGCy'C Cuts at: 0 257 307 762 783 Size: 257 50 455 21 Fragments arranged by size: 455 257 50 21 BbeI G_GCGC'C Cuts at: 0 167 215 245 783 Size: 167 48 30 538 Fragments arranged by size: 538 167 48 30 BglI GCCn_nnn'nGGC Cuts at: 0 270 783 Size: 270 513 BlpI GC'TnA_GC Cuts at: 0 724 783 Size: 724 59 Bme1580I G_kGCm'C Cuts at: 0 200 375 480 783 Size: 200 175 105 303 Fragments arranged by size: 303 200 175 105 BmrI ACTGGGnnnn_n' Cuts at: 0 378 783 Size: 378 405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 577 776 783 Size: 169 408 199 7 Fragments arranged by size: 408 199 169 7 Bpu10I CC'TnA_GC Cuts at: 0 115 783 Size: 115 668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 783 Size: 338 445 BsaI GGTCTCn'nnnn_ Cuts at: 0 625 783 Size: 625 158 BsaAI yAC'GTr Cuts at: 0 765 783 Size: 765 18 BsaHI Gr'CG_yC Cuts at: 0 164 212 242 783 Size: 164 48 30 541 Fragments arranged by size: 541 164 48 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 370 400 749 779 783 Size: 370 30 349 30 4 Fragments arranged by size: 370 349 30 30 4 BseYI C'CCAG_C Cuts at: 0 12 159 497 783 Size: 12 147 338 286 Fragments arranged by size: 338 286 147 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 533 783 Size: 533 250 BsiEI CG_ry'CG Cuts at: 0 60 783 Size: 60 723 BsiHKAI G_wGCw'C Cuts at: 0 257 783 Size: 257 526 Bsp1286I G_dGCh'C Cuts at: 0 200 257 307 375 480 762 783 Size: 200 57 50 68 105 282 21 Fragments arranged by size: 282 200 105 68 57 50 21 BsrBI CCG'CTC Cuts at: 0 75 171 783 Size: 75 96 612 Fragments arranged by size: 612 96 75 BsrFI r'CCGG_y Cuts at: 0 220 783 Size: 220 563 BssSI C'ACGA_G Cuts at: 0 527 783 Size: 527 256 BstAPI GCAn_nnn'nTGC Cuts at: 0 234 282 783 Size: 234 48 501 Fragments arranged by size: 501 234 48 BtgI C'CryG_G Cuts at: 0 48 452 638 783 Size: 48 404 186 145 Fragments arranged by size: 404 186 145 48 BtsI GCAGTG_nn' Cuts at: 0 136 783 Size: 136 647 EaeI y'GGCC_r Cuts at: 0 57 783 Size: 57 726 EagI C'GGCC_G Cuts at: 0 57 783 Size: 57 726 EcoICRI GAG'CTC Cuts at: 0 255 783 Size: 255 528 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 436 577 694 776 783 Size: 169 267 141 117 82 7 Fragments arranged by size: 267 169 141 117 82 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 491 783 Size: 491 292 EcoO109I rG'GnC_Cy Cuts at: 0 41 546 595 783 Size: 41 505 49 188 Fragments arranged by size: 505 188 49 41 HaeII r_GCGC'y Cuts at: 0 122 167 215 245 505 556 783 Size: 122 45 48 30 260 51 227 Fragments arranged by size: 260 227 122 51 48 45 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 736 768 783 Size: 736 32 15 Fragments arranged by size: 736 32 15 HincII GTy'rAC Cuts at: 0 459 783 Size: 459 324 HpaI GTT'AAC Cuts at: 0 459 783 Size: 459 324 KasI G'GCGC_C Cuts at: 0 163 211 241 783 Size: 163 48 30 542 Fragments arranged by size: 542 163 48 30 MslI CAynn'nnrTG Cuts at: 0 355 610 783 Size: 355 255 173 Fragments arranged by size: 355 255 173 MspA1I CmG'CkG Cuts at: 0 50 147 234 783 Size: 50 97 87 549 Fragments arranged by size: 549 97 87 50 NaeI GCC'GGC Cuts at: 0 222 783 Size: 222 561 NarI GG'CG_CC Cuts at: 0 164 212 242 783 Size: 164 48 30 541 Fragments arranged by size: 541 164 48 30 NgoMIV G'CCGG_C Cuts at: 0 220 783 Size: 220 563 NspI r_CATG'y Cuts at: 0 615 654 783 Size: 615 39 129 Fragments arranged by size: 615 129 39 PflMI CCAn_nnn'nTGG Cuts at: 0 549 783 Size: 549 234 PfoI T'CCnGG_A Cuts at: 0 425 783 Size: 425 358 PmlI CAC'GTG Cuts at: 0 765 783 Size: 765 18 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 370 402 783 Size: 370 32 381 Fragments arranged by size: 381 370 32 PpuMI rG'GwC_Cy Cuts at: 0 546 595 783 Size: 546 49 188 Fragments arranged by size: 546 188 49 PstI C_TGCA'G Cuts at: 0 278 668 783 Size: 278 390 115 Fragments arranged by size: 390 278 115 PvuII CAG'CTG Cuts at: 0 147 234 783 Size: 147 87 549 Fragments arranged by size: 549 147 87 SacI G_AGCT'C Cuts at: 0 257 783 Size: 257 526 SacII CC_GC'GG Cuts at: 0 51 783 Size: 51 732 SfcI C'TryA_G Cuts at: 0 274 664 783 Size: 274 390 119 Fragments arranged by size: 390 274 119 SfoI GGC'GCC Cuts at: 0 165 213 243 783 Size: 165 48 30 540 Fragments arranged by size: 540 165 48 30 SmaI CCC'GGG Cuts at: 0 628 783 Size: 628 155 SmlI C'TyrA_G Cuts at: 0 353 783 Size: 353 430 StuI AGG'CCT Cuts at: 0 421 783 Size: 421 362 StyI C'CwwG_G Cuts at: 0 266 783 Size: 266 517 TatI w'GTAC_w Cuts at: 0 506 783 Size: 506 277 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 749 781 783 Size: 749 32 2 Fragments arranged by size: 749 32 2 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 330 783 Size: 330 453 XmaI C'CCGG_G Cuts at: 0 626 783 Size: 626 157 XmnI GAAnn'nnTTC Cuts at: 0 421 783 Size: 421 362 Enzymes that do cut: AcuI AleI AloI AlwNI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstAPI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI KasI MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII SfcI SfoI SmaI SmlI StuI StyI TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI ZraI