################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AspEI AviII AvrII BamHI BbeI BfrI BlnI BseAI BsiEI BsiWI BspEI BspHI BssHII Bst1107I BstBI BstZ17I Bsu36I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I FseI FspI HpaI KasI MluI MroI NarI NheI NotI NruI NspV PacI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SbfI SfoI SfuI SgrAI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 4979 GGTACC G^GTACC AseI 7501 ATTAAT AT^TAAT AsnI 7501 ATTAAT AT^TAAT Asp718I 4979 GGTACC G^GTACC BclI 5931 TGATCA T^GATCA BspDI 1988 ATCGAT AT^CGAT ClaI 1988 ATCGAT AT^CGAT DraI 7335 TTTAAA TTT^AAA EcoRI 5216 GAATTC G^AATTC EcoRV 5125 GATATC GAT^ATC FbaI 5931 TGATCA T^GATCA HaeII 4327 AGCGCC [GA]GCGC^[CT] HgaI 6550 GACGC GACGC(5/10) HincII 6562 GTCGAC GT[CT]^[GA]AC HindII 6562 GTCGAC GT[CT]^[GA]AC KpnI 4979 GGTACC GGTAC^C NaeI 4012 GCCGGC GCC^GGC NdeI 3187 CATATG CA^TATG NgoMIV 4012 GCCGGC G^CCGGC PaeR7I 7016 CTCGAG C^TCGAG PshBI 7501 ATTAAT AT^TAAT SacI 5596 GAGCTC GAGCT^C SalI 6562 GTCGAC G^TCGAC SexAI 7255 ACCAGGT A^CC[AT]GGT SfiI 307 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 756 TACGTA TAC^GTA SphI 3650 GCATGC GCATG^C SspI 3163 AATATT AAT^ATT XhoI 7016 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 44 enzymes) # ################################### AatII 3392 GACGTC GACGT^C 3589 GACGTC AccII 4786 CGCG CG^CG 7060 CGCG Alw44I 4491 GTGCAC G^TGCAC 4587 GTGCAC ApaI 315 GGGCCC GGGCC^C 2491 GGGCCC ApaLI 4491 GTGCAC G^TGCAC 4587 GTGCAC Asp700I 1245 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3138 GAAAAGCTTC BbrPI 4494 CACGTG CAC^GTG 6195 CACGTG BbvCI 2480 CCTCAGC CCTCAGC(-5/-2) 7568 CCTCAGC BciVI 6337 GGATAC GTATCC(6/5) 6579 GGATAC BlpI 6731 GCTCAGC GC^T[ACGT]AGC 7010 GCTCAGC Bpu1102I 6731 GCTCAGC GC^T[ACGT]AGC 7010 GCTCAGC BsmBI 5073 CGTCTC CGTCTC(1/5) 2444 GAGACG Bsp1407I 42 TGTACA T^GTACA 784 TGTACA BspLU11I 1355 ACATGT A^CATGT 3242 ACATGT BsrFI 4012 GCCGGC [GA]^CCGG[CT] 5968 ACCGGC BsrGI 42 TGTACA T^GTACA 784 TGTACA BstEII 350 GGTCACC G^GT[ACGT]ACC 5551 GGTGACC BstPI 350 GGTCACC G^GT[ACGT]ACC 5551 GGTGACC BstUI 4786 CGCG CG^CG 7060 CGCG BstXI 6786 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 7400 CCATCATCCTGG CelII 6731 GCTCAGC GC^T[ACGT]AGC 7010 GCTCAGC CfoI 137 GCGC GCG^C 4328 GCGC Cfr10I 4012 GCCGGC [GA]^CCGG[CT] 5968 ACCGGC DrdI 1371 GACTGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7044 GACTGACCTGTC EcoO65I 350 GGTCACC G^GT[ACGT]ACC 5551 GGTGACC HhaI 137 GCGC GCG^C 4328 GCGC HinP1I 137 GCGC G^CGC 4328 GCGC KspI 4785 CCGCGG CCGC^GG 7059 CCGCGG MfeI 3305 CAATTG C^AATTG 3494 CAATTG MunI 3305 CAATTG C^AATTG 3494 CAATTG MvnI 4786 CGCG CG^CG 7060 CGCG PflMI 3774 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5486 CCACTGCCTGG PmaCI 4494 CACGTG CAC^GTG 6195 CACGTG PmlI 4494 CACGTG CAC^GTG 6195 CACGTG SacII 4785 CCGCGG CCGC^GG 7059 CCGCGG SapI 2133 GAAGAGC GCTCTTC(1/4) 7271 GAAGAGC ScaI 5134 AGTACT AGT^ACT 5936 AGTACT SmaI 2797 CCCGGG CCC^GGG 6435 CCCGGG SspBI 42 TGTACA T^GTACA 784 TGTACA Van91I 3774 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5486 CCACTGCCTGG XbaI 3579 TCTAGA T^CTAGA 7032 TCTAGA XmaCI 2797 CCCGGG C^CCGGG 6435 CCCGGG XmaI 2797 CCCGGG C^CCGGG 6435 CCCGGG XmnI 1245 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3138 GAAAAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII Hin1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII