(Circular) (Six-base) MAPSORT of: pFN21AE2561.seq Check: 6382 from: 1 to: 12358 pFN21AE2561 12358 bp With 182 enzymes: SgfI * March 4, 2015 12:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4694 8906 4694 Size: 4212 8146 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5401 5598 10168 278 Size: 53 83 186 936 3865 197 4570 2468 Fragments arranged by size: 4570 3865 2468 936 197 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 4113 8568 9701 1937 Size: 942 1234 4455 1133 4594 Fragments arranged by size: 4594 4455 1234 1133 942 Acc65I G'GTAC_C Cuts at: 6984 9679 6984 Size: 2695 9663 AclI AA'CG_TT Cuts at: 1563 10485 10858 1563 Size: 8922 373 3063 Fragments arranged by size: 8922 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2406 2667 4157 5654 6121 7445 9636 10421 Size: 774 261 1490 1497 467 1324 2191 785 Cuts at: 10421 11805 12073 1632 Size: 1384 268 1917 Fragments arranged by size: 2191 1917 1497 1490 1384 1324 785 774 467 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12161 AflIII A'CryG_T Cuts at: 1203 3083 3360 5247 8199 8201 11257 12234 1203 Size: 1880 277 1887 2952 2 3056 977 1327 Fragments arranged by size: 3056 2952 1887 1880 1327 977 277 2 AgeI A'CCGG_T Cuts at: 11189 11189 Size: 12358 AhdI GACnn_n'nnGTC Cuts at: 11087 11087 Size: 12358 AleI CACnn'nnGTG Cuts at: 1166 4034 4107 4688 6113 8249 8454 1166 Size: 2868 73 581 1425 2136 205 5070 Fragments arranged by size: 5070 2868 2136 1425 581 205 73 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2519 4783 7607 8565 9209 1184 Size: 1335 2264 2824 958 644 4333 Fragments arranged by size: 4333 2824 2264 1335 958 644 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7382 7414 7382 Size: 32 12326 AlwNI CAG_nnn'CTG Cuts at: 3883 5233 7854 8956 10101 11673 3883 Size: 1350 2621 1102 1145 1572 4568 Fragments arranged by size: 4568 2621 1572 1350 1145 1102 ApaI G_GGCC'C Cuts at: 2324 4500 2324 Size: 2176 10182 ApaLI G'TGCA_C Cuts at: 6496 6592 10415 11571 6496 Size: 96 3823 1156 7283 Fragments arranged by size: 7283 3823 1156 96 ApoI r'AATT_y Cuts at: 1493 2053 2623 2941 3936 5802 6707 7186 7221 Size: 560 570 318 995 1866 905 479 35 Cuts at: 7221 7268 9667 9893 10045 12154 1493 Size: 47 2399 226 152 2109 1697 Fragments arranged by size: 2399 2109 1866 1697 995 905 570 560 479 318 226 152 47 35 AseI AT'TA_AT Cuts at: 160 1032 9507 10912 160 Size: 872 8475 1405 1606 Fragments arranged by size: 8475 1606 1405 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12358 AvaI C'yCGr_G Cuts at: 1870 1943 4802 6936 7699 8440 9021 9683 1870 Size: 73 2859 2134 763 741 581 662 4545 Fragments arranged by size: 4545 2859 2134 763 741 662 581 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 8577 8610 8577 Size: 33 12325 BamHI G'GATC_C Cuts at: 9688 10055 9688 Size: 367 11991 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4676 5970 6984 9068 9185 Size: 325 217 627 2889 1294 1014 2084 117 Cuts at: 9185 9679 11134 618 Size: 494 1455 1842 Fragments arranged by size: 2889 2084 1842 1455 1294 1014 627 494 325 217 117 BanII G_rGCy'C Cuts at: 1391 2324 4086 4500 7605 8084 9677 1391 Size: 933 1762 414 3105 479 1593 4072 Fragments arranged by size: 4072 3105 1762 1593 933 479 414 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5229 5956 6309 7508 8857 928 Size: 971 3330 727 353 1199 1349 4429 Fragments arranged by size: 4429 3330 1349 1199 971 727 353 BbvCI CC'TCA_GC Cuts at: 4486 9574 4486 Size: 5088 7270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2721 2755 3007 3027 3041 3061 7534 Size: 34 784 34 252 20 14 20 4473 Cuts at: 7534 7568 10549 10583 1903 Size: 34 2981 34 3678 Fragments arranged by size: 4473 3678 2981 784 252 34 34 34 34 20 20 14 BciVI GTATCCnnnnn_n' Cuts at: 8336 8578 10250 11460 8336 Size: 242 1672 1210 9234 Fragments arranged by size: 9234 1672 1210 242 BclI T'GATC_A Cuts at: 1548 7936 1548 Size: 6388 5970 BfrBI ATG'CAT Cuts at: 3048 4296 4659 5659 5925 3048 Size: 1248 363 1000 266 9481 Fragments arranged by size: 9481 1248 1000 363 266 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2319 2802 4281 Size: 107 122 71 631 605 647 483 1479 Cuts at: 4281 4437 8657 8717 10969 136 Size: 156 4220 60 2252 1525 Fragments arranged by size: 4220 2252 1525 1479 647 631 605 483 156 122 107 71 60 BglII A'GATC_T Cuts at: 3599 5221 6871 12353 3599 Size: 1622 1650 5482 3604 Fragments arranged by size: 5482 3604 1650 1622 BlpI GC'TnA_GC Cuts at: 8737 9016 9775 8737 Size: 279 759 11320 Fragments arranged by size: 11320 759 279 Bme1580I G_kGCm'C Cuts at: 1790 2168 2324 2342 4031 4153 4500 4679 6500 Size: 378 156 18 1689 122 347 179 1821 Cuts at: 6500 6596 8315 8361 10419 11575 1790 Size: 96 1719 46 2058 1156 2573 Fragments arranged by size: 2573 2058 1821 1719 1689 1156 378 347 179 156 122 96 46 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4034 4318 4749 Size: 416 53 83 399 24 2617 284 431 Cuts at: 4749 6738 6829 6958 7325 8630 9429 10089 11047 Size: 1989 91 129 367 1305 799 660 958 Cuts at: 11047 442 Size: 1753 Fragments arranged by size: 2617 1989 1753 1305 958 799 660 431 416 399 367 284 129 91 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12358 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2572 4062 9562 2572 Size: 1490 5500 5368 Fragments arranged by size: 5500 5368 1490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2973 3717 4078 4614 4957 5639 6666 7914 Size: 1734 744 361 536 343 682 1027 1248 Cuts at: 7914 8771 11018 1239 Size: 857 2247 2579 Fragments arranged by size: 2579 2247 1734 1248 1027 857 744 682 536 361 343 Bpu10I CC'TnA_GC Cuts at: 4249 4486 5693 9574 4249 Size: 237 1207 3881 7033 Fragments arranged by size: 7033 3881 1207 237 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 9650 9667 10483 11348 11646 9650 Size: 17 816 865 298 10362 Fragments arranged by size: 10362 865 816 298 17 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5660 6149 8326 8747 9104 9251 11021 Size: 617 4161 489 2177 421 357 147 1770 Cuts at: 11021 882 Size: 2219 Fragments arranged by size: 4161 2219 2177 1770 617 489 421 357 147 BsaAI yAC'GTr Cuts at: 493 1204 2763 3067 6501 8202 10149 493 Size: 711 1559 304 3434 1701 1947 2702 Fragments arranged by size: 3434 2702 1947 1701 1559 711 304 BsaBI GATnn'nnATC Cuts at: 3335 5366 7841 12352 3335 Size: 2031 2475 4511 3341 Fragments arranged by size: 4511 3341 2475 2031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5398 5595 8556 10165 Size: 53 83 186 936 3865 197 2961 1609 Cuts at: 10165 10547 11182 275 Size: 382 635 1451 Fragments arranged by size: 3865 2961 1609 1451 936 635 382 197 186 83 53 BsaWI w'CCGG_w Cuts at: 5601 7952 9462 10791 11189 11463 11610 12021 5601 Size: 2351 1510 1329 398 274 147 411 5938 Fragments arranged by size: 5938 2351 1510 1329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4051 4081 4456 4486 7592 Size: 30 165 30 2648 30 375 30 3106 Cuts at: 7592 7622 8687 8717 9248 9278 9562 9592 1178 Size: 30 1065 30 531 30 284 30 3944 Fragments arranged by size: 3944 3106 2648 1065 531 375 284 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4319 5603 5636 5857 5932 6288 1186 Size: 3133 1284 33 221 75 356 7256 Fragments arranged by size: 7256 3133 1284 356 221 75 33 BseYI C'CCAG_C Cuts at: 2065 2316 2477 3208 3789 5674 7730 9097 9205 Size: 251 161 731 581 1885 2056 1367 108 Cuts at: 9205 11561 12057 2065 Size: 2356 496 2366 Fragments arranged by size: 2366 2356 2056 1885 1367 731 581 496 251 161 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2622 2983 3482 4287 4526 4779 5630 Size: 573 886 361 499 805 239 253 851 Cuts at: 5630 6145 6394 6399 6646 7613 7846 8350 8406 Size: 515 249 5 247 967 233 504 56 Cuts at: 8406 8878 1163 Size: 472 4643 Fragments arranged by size: 4643 967 886 851 805 573 515 504 499 472 361 253 249 247 239 233 56 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9808 10569 11597 1540 Size: 275 186 7807 761 1028 2301 Fragments arranged by size: 7807 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6500 6596 7091 7605 8418 8738 9138 9569 10419 Size: 96 495 514 813 320 400 431 850 Cuts at: 10419 10504 11575 6500 Size: 85 1071 7283 Fragments arranged by size: 7283 1071 850 813 514 495 431 400 320 96 85 BsmI GAATG_Cn' Cuts at: 2672 3013 3050 4661 5500 6014 6061 6589 9869 Size: 341 37 1611 839 514 47 528 3280 Cuts at: 9869 9962 2672 Size: 93 5068 Fragments arranged by size: 5068 3280 1611 839 528 514 341 93 47 37 BsmBI CGTCTCn'nnnn_ Cuts at: 4443 7084 4443 Size: 2641 9717 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2324 2342 4031 4086 4153 4500 Size: 399 378 156 18 1689 55 67 347 Cuts at: 4500 4679 6500 6596 7091 7605 8084 8315 8361 Size: 179 1821 96 495 514 479 231 46 Cuts at: 8361 8418 8738 9138 9569 9677 10419 10504 11575 Size: 57 320 400 431 108 742 85 1071 Cuts at: 11575 1391 Size: 2174 Fragments arranged by size: 2174 1821 1689 1071 742 514 495 479 431 400 399 378 347 320 231 179 156 108 96 85 67 57 55 46 18 BspEI T'CCGG_A Cuts at: 12021 12021 Size: 12358 BspHI T'CATG_A Cuts at: 10247 11977 10247 Size: 1730 10628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 2417 4113 4694 5430 5652 5888 6167 Size: 1364 209 1696 581 736 222 236 279 Cuts at: 6167 6794 7384 7589 7794 8906 8941 8952 9713 Size: 627 590 205 205 1112 35 11 761 Cuts at: 9713 844 Size: 3489 Fragments arranged by size: 3489 1696 1364 1112 761 736 627 590 581 279 236 222 209 205 205 35 11 BsrBI CCG'CTC Cuts at: 1394 1823 2351 4143 7822 10245 1394 Size: 429 528 1792 3679 2423 3507 Fragments arranged by size: 3679 3507 2423 1792 528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2371 3477 3906 4168 4549 4654 6319 Size: 1183 1123 1106 429 262 381 105 1665 Cuts at: 6319 6445 9643 10853 11027 65 Size: 126 3198 1210 174 1396 Fragments arranged by size: 3198 1665 1396 1210 1183 1123 1106 429 381 262 174 126 105 BsrFI r'CCGG_y Cuts at: 1700 1810 6017 7973 11002 11189 1700 Size: 110 4207 1956 3029 187 2869 Fragments arranged by size: 4207 3029 2869 1956 187 110 BsrGI T'GTAC_A Cuts at: 96 2047 2789 96 Size: 1951 742 9665 Fragments arranged by size: 9665 1951 742 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12358 BssSI C'ACGA_G Cuts at: 5099 8122 8411 10418 11430 5099 Size: 3023 289 2007 1012 6027 Fragments arranged by size: 6027 3023 2007 1012 289 BstAPI GCAn_nnn'nTGC Cuts at: 794 2706 6150 6422 6534 9353 794 Size: 1912 3444 272 112 2819 3799 Fragments arranged by size: 3799 3444 2819 1912 272 112 BstBI TT'CG_AA Cuts at: 11162 11162 Size: 12358 BstEII G'GTnAC_C Cuts at: 1184 2355 7556 1184 Size: 1171 5201 5986 Fragments arranged by size: 5986 5201 1171 BstXI CCAn_nnnn'nTGG Cuts at: 8798 9412 8798 Size: 614 11744 BstYI r'GATC_y Cuts at: 1972 3599 3740 5221 6871 7477 7842 9393 9688 Size: 1627 141 1481 1650 606 365 1551 295 Cuts at: 9688 10055 10440 10457 11909 11995 12007 12353 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1650 1627 1551 1481 1452 606 385 367 365 346 295 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2809 3436 3516 5779 6211 6565 6790 Size: 552 1744 627 80 2263 432 354 225 Cuts at: 6790 8053 8682 9064 513 Size: 1263 629 382 3807 Fragments arranged by size: 3807 2263 1744 1263 629 627 552 432 382 354 225 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2550 2709 4434 8079 487 Size: 683 56 1324 159 1725 3645 4766 Fragments arranged by size: 4766 3645 1725 1324 683 159 56 BtsI GCAGTG_nn' Cuts at: 2914 3918 5004 7491 8096 8157 8549 8941 9225 Size: 1004 1086 2487 605 61 392 392 284 Cuts at: 9225 9296 9874 10668 10688 2914 Size: 71 578 794 20 4584 Fragments arranged by size: 4584 2487 1086 1004 794 605 578 392 392 284 71 61 20 ClaI AT'CG_AT Cuts at: 1554 3994 1554 Size: 2440 9918 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3876 3911 529 Size: 35 3312 35 8976 Fragments arranged by size: 8976 3312 35 35 DraI TTT'AAA Cuts at: 9342 9662 9342 Size: 320 12038 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3382 9055 11365 809 Size: 507 2066 5673 2310 1802 Fragments arranged by size: 5673 2310 2066 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2311 2818 3194 Size: 54 1425 50 275 18 481 507 376 Cuts at: 3194 3801 3837 3931 7573 7947 8006 8292 9805 Size: 607 36 94 3642 374 59 286 1513 Cuts at: 9805 10694 12323 8 Size: 889 1629 43 Fragments arranged by size: 3642 1629 1513 1425 889 607 507 481 376 374 286 275 94 59 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 9805 1537 Size: 275 7993 4090 Fragments arranged by size: 7993 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2115 4100 4133 5909 7346 9271 9492 10288 Size: 762 1985 33 1776 1437 1925 221 796 Cuts at: 10288 1353 Size: 3423 Fragments arranged by size: 3423 1985 1925 1776 1437 796 762 221 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10945 11319 11465 387 Size: 1064 138 9356 374 146 1280 Fragments arranged by size: 9356 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 7603 7603 Size: 12358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2406 2667 2973 3717 4078 4157 4614 Size: 393 774 261 306 744 361 79 457 Cuts at: 4614 4957 5639 5654 6121 6666 7445 7914 8771 Size: 343 682 15 467 545 779 469 857 Cuts at: 8771 9636 10421 11018 11805 12073 1239 Size: 865 785 597 787 268 1524 Fragments arranged by size: 1524 865 857 787 785 779 774 744 682 597 545 469 467 457 393 361 343 306 268 261 79 15 EcoNI CCTnn'n_nnAGG Cuts at: 4617 7795 8948 4617 Size: 3178 1153 8027 Fragments arranged by size: 8027 3178 1153 EcoO109I rG'GnC_Cy Cuts at: 2320 3212 4425 4497 4799 5319 6230 6605 6887 Size: 892 1213 72 302 520 911 375 282 Cuts at: 6887 8117 8953 9238 2320 Size: 1230 836 285 5440 Fragments arranged by size: 5440 1230 1213 911 892 836 520 375 302 285 282 72 EcoRI G'AATT_C Cuts at: 7221 9667 10045 7221 Size: 2446 378 9534 Fragments arranged by size: 9534 2446 378 EcoRV GAT'ATC Cuts at: 7132 7132 Size: 12358 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5593 7882 8656 5593 Size: 2289 774 9295 Fragments arranged by size: 9295 2289 774 FspI TGC'GCA Cuts at: 10864 10864 Size: 12358 HaeII r_GCGC'y Cuts at: 1678 6336 11505 1678 Size: 4658 5169 2531 Fragments arranged by size: 5169 4658 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2120 2122 2152 4051 Size: 32 135 32 518 30 2 30 1899 Cuts at: 4051 4083 4766 4798 7928 7960 8912 8944 9101 Size: 32 683 32 3130 32 952 32 157 Cuts at: 9101 9133 9378 9410 10999 11031 11073 11105 1373 Size: 32 245 32 1589 32 42 32 2626 Fragments arranged by size: 3130 2626 1899 1589 952 683 518 245 157 135 42 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 8569 9702 9948 669 Size: 988 281 6631 1133 246 3079 Fragments arranged by size: 6631 3079 1133 988 281 246 HindIII A'AGCT_T Cuts at: 748 3183 3425 3749 5146 748 Size: 2435 242 324 1397 7960 Fragments arranged by size: 7960 2435 1397 324 242 HpaI GTT'AAC Cuts at: 9948 9948 Size: 12358 KpnI G_GTAC'C Cuts at: 6988 9683 6988 Size: 2695 9663 MfeI C'AATT_G Cuts at: 5310 5499 9957 5310 Size: 189 4458 7711 Fragments arranged by size: 7711 4458 189 MluI A'CGCG_T Cuts at: 12234 12234 Size: 12358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2317 4685 8458 9033 9271 9474 11472 11656 Size: 1012 2368 3773 575 238 203 1998 184 Cuts at: 11656 1305 Size: 2007 Fragments arranged by size: 3773 2368 2007 1998 1012 575 238 203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2820 3196 3803 3839 7575 Size: 54 1425 343 988 376 607 36 3736 Cuts at: 7575 8294 10 Size: 719 4074 Fragments arranged by size: 4074 3736 1425 988 719 607 376 343 54 36 MslI CAynn'nnrTG Cuts at: 518 1166 2491 2748 3006 3359 3996 4034 4105 Size: 648 1325 257 258 353 637 38 71 Cuts at: 4105 4107 4688 6113 6373 8249 8454 9413 10316 Size: 2 581 1425 260 1876 205 959 903 Cuts at: 10316 10675 10834 518 Size: 359 159 2042 Fragments arranged by size: 2042 1876 1425 1325 959 903 648 637 581 359 353 260 258 257 205 159 71 38 2 MspA1I CmG'CkG Cuts at: 1606 2069 2316 2481 6141 6792 7009 7597 7671 Size: 463 247 165 3660 651 217 588 74 Cuts at: 7671 7734 8331 8364 8509 8523 9066 9774 10451 Size: 63 597 33 145 14 543 708 677 Cuts at: 10451 11599 11844 1606 Size: 1148 245 2120 Fragments arranged by size: 3660 2120 1148 708 677 651 597 588 543 463 247 245 217 165 145 74 63 33 14 NaeI GCC'GGC Cuts at: 6019 6019 Size: 12358 NcoI C'CATG_G Cuts at: 513 1065 3436 3516 5779 6211 6565 8682 513 Size: 552 2371 80 2263 432 354 2117 4189 Fragments arranged by size: 4189 2371 2263 2117 552 432 354 80 NdeI CA'TA_TG Cuts at: 387 5193 387 Size: 4806 7552 NgoMIV G'CCGG_C Cuts at: 6017 6017 Size: 12358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12358 NotI GC'GGCC_GC Cuts at: 9805 9805 Size: 12358 NsiI A_TGCA'T Cuts at: 3050 4298 4661 5661 5927 3050 Size: 1248 363 1000 266 9481 Fragments arranged by size: 9481 1248 1000 363 266 NspI r_CATG'y Cuts at: 1132 1229 2796 3364 3632 4296 4401 4446 5251 Size: 97 1567 568 268 664 105 45 805 Cuts at: 5251 5659 5865 6201 6324 8244 9160 9716 11261 Size: 408 206 336 123 1920 916 556 1545 Cuts at: 11261 1132 Size: 2229 Fragments arranged by size: 2229 1920 1567 1545 916 805 664 568 556 408 336 268 206 123 105 97 45 PasI CC'CwG_GG Cuts at: 1348 7660 1348 Size: 6312 6046 PciI A'CATG_T Cuts at: 3360 5247 11257 3360 Size: 1887 6010 4461 Fragments arranged by size: 6010 4461 1887 PflMI CCAn_nnn'nTGG Cuts at: 1108 5785 7497 1108 Size: 4677 1712 5969 Fragments arranged by size: 5969 4677 1712 PfoI T'CCnGG_A Cuts at: 4225 4630 5616 8580 4225 Size: 405 986 2964 8003 Fragments arranged by size: 8003 2964 986 405 PmeI GTTT'AAAC Cuts at: 9662 9662 Size: 12358 PmlI CAC'GTG Cuts at: 6501 8202 6501 Size: 1701 10657 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2230 2262 5884 5916 8786 8818 10427 10459 11940 Size: 32 3622 32 2870 32 1609 32 1481 Cuts at: 11940 11972 2230 Size: 32 2616 Fragments arranged by size: 3622 2870 2616 1609 1481 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3212 4799 6230 6605 8953 9238 3212 Size: 1587 1431 375 2348 285 6332 Fragments arranged by size: 6332 2348 1587 1431 375 285 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12358 PsiI TTA'TAA Cuts at: 9928 9928 Size: 12358 PspOMI G'GGCC_C Cuts at: 2320 4496 2320 Size: 2176 10182 PspXI vC'TCGA_Gb Cuts at: 9021 9021 Size: 12358 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3578 3610 5983 6015 3578 Size: 32 2373 32 9921 Fragments arranged by size: 9921 2373 32 32 PstI C_TGCA'G Cuts at: 830 5229 6919 7586 8152 8330 8952 9291 9710 Size: 4399 1690 667 566 178 622 339 419 Cuts at: 9710 830 Size: 3478 Fragments arranged by size: 4399 3478 1690 667 622 566 419 339 178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12358 PvuII CAG'CTG Cuts at: 2069 2481 7009 7734 8331 8364 8509 2069 Size: 412 4528 725 597 33 145 5918 Fragments arranged by size: 5918 4528 725 597 412 145 33 SacI G_AGCT'C Cuts at: 7605 7605 Size: 12358 SacII CC_GC'GG Cuts at: 6793 9067 6793 Size: 2274 10084 SalI G'TCGA_C Cuts at: 1936 8567 9700 1936 Size: 6631 1133 4594 Fragments arranged by size: 6631 4594 1133 SanDI GG'GwC_CC Cuts at: 3212 4799 3212 Size: 1587 10771 SapI GCTCTTCn'nnn_ Cuts at: 4133 9271 4133 Size: 5138 7220 SbfI CC_TGCA'GG Cuts at: 9710 9710 Size: 12358 ScaI AGT'ACT Cuts at: 7141 7943 10606 12318 7141 Size: 802 2663 1712 7181 Fragments arranged by size: 7181 2663 1712 802 SexAI A'CCwGG_T Cuts at: 9260 9260 Size: 12358 SfcI C'TryA_G Cuts at: 826 1045 2199 2880 3234 4114 4639 5225 6700 Size: 219 1154 681 354 880 525 586 1475 Cuts at: 6700 6915 7582 8148 8326 8948 9287 9706 10841 Size: 215 667 566 178 622 339 419 1135 Cuts at: 10841 11522 11713 826 Size: 681 191 1471 Fragments arranged by size: 1475 1471 1154 1135 880 681 681 667 622 586 566 525 419 354 339 219 215 191 178 SfiI GGCCn_nnn'nGGCC Cuts at: 2319 2319 Size: 12358 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12358 SmaI CCC'GGG Cuts at: 1872 4804 8442 9685 1872 Size: 2932 3638 1243 4545 Fragments arranged by size: 4545 3638 2932 1243 SmlI C'TyrA_G Cuts at: 820 1017 1943 9021 9629 9646 10462 11363 11625 Size: 197 926 7078 608 17 816 901 262 Cuts at: 11625 820 Size: 1553 Fragments arranged by size: 7078 1553 926 901 816 608 262 197 17 SnaBI TAC'GTA Cuts at: 493 2763 10149 493 Size: 2270 7386 2702 Fragments arranged by size: 7386 2702 2270 SpeI A'CTAG_T Cuts at: 152 152 Size: 12358 SphI G_CATG'C Cuts at: 1132 5659 9716 1132 Size: 4527 4057 3774 Fragments arranged by size: 4527 4057 3774 SrfI GCCC'GGGC Cuts at: 8442 8442 Size: 12358 SspI AAT'ATT Cuts at: 5 52 5170 10282 5 Size: 47 5118 5112 2081 Fragments arranged by size: 5118 5112 2081 47 StuI AGG'CCT Cuts at: 1517 7886 8660 9337 1517 Size: 6369 774 677 4538 Fragments arranged by size: 6369 4538 774 677 StyI C'CwwG_G Cuts at: 513 1065 2152 3436 3516 3581 4315 5779 6211 Size: 552 1087 1284 80 65 734 1464 432 Cuts at: 6211 6226 6234 6391 6456 6565 7054 7461 7739 Size: 15 8 157 65 109 489 407 278 Cuts at: 7739 8682 9432 9797 513 Size: 943 750 365 3074 Fragments arranged by size: 3074 1464 1284 1087 943 750 734 552 489 432 407 365 278 157 109 80 65 65 15 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2141 3059 4694 5958 7607 10385 10402 Size: 1255 561 918 1635 1264 1649 2778 17 Cuts at: 10402 10555 10740 11185 325 Size: 153 185 445 1498 Fragments arranged by size: 2778 1649 1635 1498 1264 1255 918 561 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2047 2789 4053 Size: 274 80 33 51 1443 70 742 1264 Cuts at: 4053 6749 7139 7941 10604 12316 96 Size: 2696 390 802 2663 1712 138 Fragments arranged by size: 2696 2663 1712 1443 1264 802 742 390 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4456 4488 4840 4872 6477 6509 7978 8010 12318 Size: 32 352 32 1605 32 1469 32 4308 Cuts at: 12318 12350 4456 Size: 32 4464 Fragments arranged by size: 4464 4308 1605 1469 352 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5233 9115 9176 1316 Size: 3917 3882 61 4498 Fragments arranged by size: 4498 3917 3882 61 XbaI T'CTAG_A Cuts at: 5584 9037 9694 5584 Size: 3453 657 8248 Fragments arranged by size: 8248 3453 657 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2426 2855 3320 2426 Size: 429 465 11464 Fragments arranged by size: 11464 465 429 XhoI C'TCGA_G Cuts at: 1943 9021 1943 Size: 7078 5280 XmaI C'CCGG_G Cuts at: 1870 4802 8440 9683 1870 Size: 2932 3638 1243 4545 Fragments arranged by size: 4545 3638 2932 1243 XmnI GAAnn'nnTTC Cuts at: 3254 5147 10487 3254 Size: 1893 5340 5125 Fragments arranged by size: 5340 5125 1893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5399 5596 10166 276 Size: 53 83 186 936 3865 197 4570 2468 Fragments arranged by size: 4570 3865 2468 936 197 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BsiWI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI PacI RsrII SfoI SwaI