(Circular) (Six-base) MAPSORT of: pFN21AE2123.seq Check: 3122 from: 1 to: 7447 pFN21AE2123 7447 bp With 182 enzymes: SgfI * February 3, 2015 17:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3353 4378 3353 Size: 1025 6422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5257 278 Size: 53 83 186 936 3721 2468 Fragments arranged by size: 3721 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4790 1937 Size: 2853 4594 Acc65I G'GTAC_C Cuts at: 4768 4768 Size: 7447 AclI AA'CG_TT Cuts at: 1563 5574 5947 1563 Size: 4011 373 3063 Fragments arranged by size: 4011 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3515 4598 4664 5510 6894 7162 1632 Size: 1883 1083 66 846 1384 268 1917 Fragments arranged by size: 1917 1883 1384 1083 846 268 66 AfeI AGC'GCT Cuts at: 2288 4004 4334 2288 Size: 1716 330 5401 Fragments arranged by size: 5401 1716 330 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7250 AflIII A'CryG_T Cuts at: 1203 4440 6346 7323 1203 Size: 3237 1906 977 1327 Fragments arranged by size: 3237 1906 1327 977 AgeI A'CCGG_T Cuts at: 6278 6278 Size: 7447 AhdI GACnn_n'nnGTC Cuts at: 6176 6176 Size: 7447 AleI CACnn'nnGTG Cuts at: 1166 4307 1166 Size: 3141 4306 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7447 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 4199 4231 2468 Size: 32 1699 32 5684 Fragments arranged by size: 5684 1699 32 32 AlwNI CAG_nnn'CTG Cuts at: 2473 2777 3547 5190 6762 2473 Size: 304 770 1643 1572 3158 Fragments arranged by size: 3158 1643 1572 770 304 ApaLI G'TGCA_C Cuts at: 4130 5504 6660 4130 Size: 1374 1156 4917 Fragments arranged by size: 4917 1374 1156 ApoI r'AATT_y Cuts at: 1493 4756 4982 5134 7243 1493 Size: 3263 226 152 2109 1697 Fragments arranged by size: 3263 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6001 160 Size: 872 4969 1606 Fragments arranged by size: 4969 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 AvaI C'yCGr_G Cuts at: 1870 1943 2294 2822 2844 3350 4772 1870 Size: 73 351 528 22 506 1422 4545 Fragments arranged by size: 4545 1422 528 506 351 73 22 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4387 4420 4387 Size: 33 7414 BamHI G'GATC_C Cuts at: 3028 3426 4777 5144 3028 Size: 398 1351 367 5331 Fragments arranged by size: 5331 1351 398 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2389 2609 3220 3283 Size: 325 217 627 261 341 220 611 63 Cuts at: 3283 3611 3689 4768 6223 618 Size: 328 78 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 611 341 328 325 261 220 217 78 63 BanII G_rGCy'C Cuts at: 1391 2072 2192 2823 2949 3242 3655 3945 4038 Size: 681 120 631 126 293 413 290 93 Cuts at: 4038 4350 4766 1391 Size: 312 416 4072 Fragments arranged by size: 4072 681 631 416 413 312 293 290 126 120 93 BbeI G_GCGC'C Cuts at: 2052 3287 2052 Size: 1235 6212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3506 3516 4594 928 Size: 971 1607 10 1078 3781 Fragments arranged by size: 3781 1607 1078 971 10 BbvCI CC'TCA_GC Cuts at: 4097 4291 4097 Size: 194 7253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5638 5672 1903 Size: 34 3701 34 3678 Fragments arranged by size: 3701 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5339 6549 5339 Size: 1210 6237 BclI T'GATC_A Cuts at: 1548 2217 1548 Size: 669 6778 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 2516 3097 Size: 107 122 71 631 605 358 486 581 Cuts at: 3097 6058 136 Size: 2961 1525 Fragments arranged by size: 2961 1525 631 605 581 486 358 122 107 71 BglII A'GATC_T Cuts at: 3166 3555 7442 3166 Size: 389 3887 3171 Fragments arranged by size: 3887 3171 389 BlpI GC'TnA_GC Cuts at: 3902 4124 4264 4864 3902 Size: 222 140 600 6485 Fragments arranged by size: 6485 600 222 140 Bme1580I G_kGCm'C Cuts at: 1790 2612 2728 3225 3614 3692 3710 4134 5508 Size: 822 116 497 389 78 18 424 1374 Cuts at: 5508 6664 1790 Size: 1156 2573 Fragments arranged by size: 2573 1374 1156 822 497 424 389 116 78 18 BmgBI CAC'GTC Cuts at: 1318 3504 4159 4426 1318 Size: 2186 655 267 4339 Fragments arranged by size: 4339 2186 655 267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2852 3304 5178 Size: 416 53 83 399 24 1435 452 1874 Cuts at: 5178 6136 442 Size: 958 1753 Fragments arranged by size: 1874 1753 1435 958 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2831 1056 Size: 1775 5672 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3577 3631 3577 Size: 54 7393 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2387 2735 2768 2799 2853 2889 2913 Size: 890 258 348 33 31 54 36 24 Cuts at: 2913 2933 2943 2973 3003 3051 3077 3147 3653 Size: 20 10 30 30 48 26 70 506 Cuts at: 3653 3959 4027 4255 4342 4538 6107 1239 Size: 306 68 228 87 196 1569 2579 Fragments arranged by size: 2579 1569 890 506 348 306 258 228 196 87 70 68 54 48 36 33 31 30 30 26 24 20 10 Bpu10I CC'TnA_GC Cuts at: 4079 4097 4291 4079 Size: 18 194 7235 Fragments arranged by size: 7235 194 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 3416 4709 5572 6437 6735 2651 Size: 765 1293 863 865 298 3363 Fragments arranged by size: 3363 1293 865 863 765 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 6110 882 Size: 617 2742 1869 2219 Fragments arranged by size: 2742 2219 1869 617 BsaAI yAC'GTr Cuts at: 493 1204 2686 5238 493 Size: 711 1482 2552 2702 Fragments arranged by size: 2702 2552 1482 711 BsaBI GATnn'nnATC Cuts at: 4172 7441 4172 Size: 3269 4178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2097 2118 2508 Size: 53 83 186 936 516 48 21 390 Cuts at: 2508 3284 5254 5636 6271 275 Size: 776 1970 382 635 1451 Fragments arranged by size: 1970 1451 936 776 635 516 390 382 186 83 53 48 21 BsaWI w'CCGG_w Cuts at: 2650 2941 3531 4569 5880 6278 6552 6699 7110 Size: 291 590 1038 1311 398 274 147 411 Cuts at: 7110 2650 Size: 2987 Fragments arranged by size: 2987 1311 1038 590 411 398 291 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 2887 2917 4346 Size: 30 165 30 822 30 632 30 1429 Cuts at: 4346 4376 4659 4689 1178 Size: 30 283 30 3936 Fragments arranged by size: 3936 1429 822 632 283 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 2512 2587 3149 3802 4135 4178 4279 Size: 1039 287 75 562 653 333 43 101 Cuts at: 4279 4535 4538 4541 1186 Size: 256 3 3 4092 Fragments arranged by size: 4092 1039 653 562 333 287 256 101 75 43 3 3 BseYI C'CCAG_C Cuts at: 2742 2852 3252 4025 6650 7146 2742 Size: 110 400 773 2625 496 3043 Fragments arranged by size: 3043 2625 773 496 400 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 2129 2246 2276 2708 1163 Size: 573 294 99 117 30 432 5902 Fragments arranged by size: 5902 573 432 294 117 99 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3665 4015 4140 4897 5658 Size: 275 186 33 1631 350 125 757 761 Cuts at: 5658 6686 1540 Size: 1028 2301 Fragments arranged by size: 2301 1631 1028 761 757 350 275 186 125 33 BsiHKAI G_wGCw'C Cuts at: 2192 2385 2754 2823 2851 2949 3279 3399 4038 Size: 193 369 69 28 98 330 120 639 Cuts at: 4038 4134 5508 5593 6664 2192 Size: 96 1374 85 1071 2975 Fragments arranged by size: 2975 1374 1071 639 369 330 193 120 98 96 85 69 28 BsmI GAATG_Cn' Cuts at: 4958 5051 4958 Size: 93 7354 BsmBI CGTCTCn'nnnn_ Cuts at: 3665 3665 Size: 7447 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2192 2385 2612 2728 2754 2823 Size: 399 282 120 193 227 116 26 69 Cuts at: 2823 2851 2949 3225 3242 3279 3399 3614 3655 Size: 28 98 276 17 37 120 215 41 Cuts at: 3655 3692 3710 3945 4038 4134 4350 4766 5508 Size: 37 18 235 93 96 216 416 742 Cuts at: 5508 5593 6664 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 416 399 282 276 235 227 216 215 193 120 120 116 98 96 93 85 69 41 37 37 28 26 18 17 BspEI T'CCGG_A Cuts at: 2650 2941 3531 7110 2650 Size: 291 590 3579 2987 Fragments arranged by size: 3579 2987 590 291 BspHI T'CATG_A Cuts at: 5336 7066 5336 Size: 1730 5717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2487 3353 4378 4802 844 Size: 1337 306 866 1025 424 3489 Fragments arranged by size: 3489 1337 1025 866 424 306 BsrBI CCG'CTC Cuts at: 1394 1823 3898 4375 5334 1394 Size: 429 2075 477 959 3507 Fragments arranged by size: 3507 2075 959 477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5942 6116 65 Size: 1183 4694 174 1396 Fragments arranged by size: 4694 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2863 4139 6091 6278 1700 Size: 110 1053 1276 1952 187 2869 Fragments arranged by size: 2869 1952 1276 1053 187 110 BsrGI T'GTAC_A Cuts at: 96 4443 96 Size: 4347 3100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7447 BssSI C'ACGA_G Cuts at: 5507 6519 5507 Size: 1012 6435 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 794 Size: 1595 5852 BstBI TT'CG_AA Cuts at: 6251 6251 Size: 7447 BstEII G'GTnAC_C Cuts at: 1184 4459 1184 Size: 3275 4172 BstXI CCAn_nnnn'nTGG Cuts at: 3391 4309 3391 Size: 918 6529 BstYI r'GATC_y Cuts at: 1972 3028 3166 3426 3534 3555 4581 4777 5144 Size: 1056 138 260 108 21 1026 196 367 Cuts at: 5144 5529 5546 6998 7084 7096 7442 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1056 1026 385 367 346 260 196 138 108 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2778 3971 2778 Size: 1193 6254 BtgI C'CryG_G Cuts at: 513 1065 2004 2266 3891 4308 4500 513 Size: 552 939 262 1625 417 192 3460 Fragments arranged by size: 3460 1625 939 552 417 262 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3838 4096 487 Size: 683 56 2612 258 3838 Fragments arranged by size: 3838 2612 683 258 56 BtsI GCAGTG_nn' Cuts at: 3229 3738 4963 5757 5777 3229 Size: 509 1225 794 20 4899 Fragments arranged by size: 4899 1225 794 509 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7447 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7412 DraI TTT'AAA Cuts at: 4751 4751 Size: 7447 DraIII CAC_nnn'GTG Cuts at: 2389 2389 Size: 7447 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6454 809 Size: 507 5138 1802 Fragments arranged by size: 5138 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3324 3411 Size: 54 1425 50 275 18 201 1293 87 Cuts at: 3411 3662 4137 4201 4894 5783 7412 8 Size: 251 475 64 693 889 1629 43 Fragments arranged by size: 1629 1425 1293 889 693 475 275 251 201 87 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3662 4137 4894 1537 Size: 275 219 1631 475 757 4090 Fragments arranged by size: 4090 1631 757 475 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 3779 5377 1353 Size: 2426 1598 3423 Fragments arranged by size: 3423 2426 1598 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2092 6034 6408 6554 387 Size: 1064 138 503 3942 374 146 1280 Fragments arranged by size: 3942 1280 1064 503 374 146 138 EcoICRI GAG'CTC Cuts at: 2190 2821 2947 4036 2190 Size: 631 126 1089 5601 Fragments arranged by size: 5601 1089 631 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2387 2735 2768 2799 2853 2889 Size: 393 497 258 348 33 31 54 36 Cuts at: 2889 2913 2933 2943 2973 3003 3051 3077 3147 Size: 24 20 10 30 30 48 26 70 Cuts at: 3147 3515 3653 3959 4027 4255 4342 4538 4598 Size: 368 138 306 68 228 87 196 60 Cuts at: 4598 4664 5510 6107 6894 7162 1239 Size: 66 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 597 497 393 368 348 306 268 258 228 196 138 87 70 68 66 60 54 48 36 33 31 30 30 26 24 20 10 EcoNI CCTnn'n_nnAGG Cuts at: 2249 4520 2249 Size: 2271 5176 EcoO109I rG'GnC_Cy Cuts at: 2470 3876 4021 2470 Size: 1406 145 5896 Fragments arranged by size: 5896 1406 145 EcoRI G'AATT_C Cuts at: 4756 5134 4756 Size: 378 7069 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4672 4759 4672 Size: 87 7360 FspI TGC'GCA Cuts at: 2249 5953 2249 Size: 3704 3743 HaeII r_GCGC'y Cuts at: 1678 2052 2290 2722 2996 3074 3287 4006 4336 Size: 374 238 432 274 78 213 719 330 Cuts at: 4336 6594 1678 Size: 2258 2531 Fragments arranged by size: 2531 2258 719 432 374 330 274 238 213 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2612 2644 2771 Size: 32 135 32 722 32 286 32 127 Cuts at: 2771 2803 3932 3964 4659 4691 6088 6120 6162 Size: 32 1129 32 695 32 1397 32 42 Cuts at: 6162 6194 1373 Size: 32 2626 Fragments arranged by size: 2626 1397 1129 722 695 286 135 127 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2645 4791 5037 669 Size: 988 281 707 2146 246 3079 Fragments arranged by size: 3079 2146 988 707 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7447 HpaI GTT'AAC Cuts at: 5037 5037 Size: 7447 KasI G'GCGC_C Cuts at: 2048 3283 2048 Size: 1235 6212 KpnI G_GTAC'C Cuts at: 4772 4772 Size: 7447 MfeI C'AATT_G Cuts at: 5046 5046 Size: 7447 MluI A'CGCG_T Cuts at: 7323 7323 Size: 7447 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 2918 4179 6561 6745 1305 Size: 1272 341 1261 2382 184 2007 Fragments arranged by size: 2382 2007 1272 1261 341 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3326 3413 4203 10 Size: 54 1425 343 1494 87 790 3254 Fragments arranged by size: 3254 1494 1425 790 343 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 3743 4307 4457 5405 5764 5923 518 Size: 648 2577 564 150 948 359 159 2042 Fragments arranged by size: 2577 2042 948 648 564 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2059 2130 2260 2674 2746 2969 3256 4104 Size: 453 71 130 414 72 223 287 848 Cuts at: 4104 4863 5540 6688 6933 1606 Size: 759 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 759 677 453 414 287 245 223 130 72 71 NaeI GCC'GGC Cuts at: 2865 4141 2865 Size: 1276 6171 NarI GG'CG_CC Cuts at: 2049 3284 2049 Size: 1235 6212 NcoI C'CATG_G Cuts at: 513 1065 2004 4500 513 Size: 552 939 2496 3460 Fragments arranged by size: 3460 2496 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7447 NgoMIV G'CCGG_C Cuts at: 2863 4139 2863 Size: 1276 6171 NheI G'CTAG_C Cuts at: 1052 2827 1052 Size: 1775 5672 NotI GC'GGCC_GC Cuts at: 4894 4894 Size: 7447 NspI r_CATG'y Cuts at: 1132 1229 2020 2701 4444 4805 6350 1132 Size: 97 791 681 1743 361 1545 2229 Fragments arranged by size: 2229 1743 1545 791 681 361 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7447 PciI A'CATG_T Cuts at: 4440 6346 4440 Size: 1906 5541 PflMI CCAn_nnn'nTGG Cuts at: 1108 3235 4390 1108 Size: 2127 1155 4165 Fragments arranged by size: 4165 2127 1155 PfoI T'CCnGG_A Cuts at: 2472 4185 2472 Size: 1713 5734 PmeI GTTT'AAAC Cuts at: 4751 4751 Size: 7447 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5516 5548 7029 7061 3563 Size: 32 1921 32 1481 32 3949 Fragments arranged by size: 3949 1921 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2470 2470 Size: 7447 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7447 PsiI TTA'TAA Cuts at: 5017 5017 Size: 7447 PspXI vC'TCGA_Gb Cuts at: 2822 2844 2822 Size: 22 7425 PstI C_TGCA'G Cuts at: 830 3474 3831 4554 4799 830 Size: 2644 357 723 245 3478 Fragments arranged by size: 3478 2644 723 357 245 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7447 PvuII CAG'CTG Cuts at: 2130 2260 2674 2746 2969 2130 Size: 130 414 72 223 6608 Fragments arranged by size: 6608 414 223 130 72 RsrII CG'GwC_CG Cuts at: 2346 2346 Size: 7447 SacI G_AGCT'C Cuts at: 2192 2823 2949 4038 2192 Size: 631 126 1089 5601 Fragments arranged by size: 5601 1089 631 126 SalI G'TCGA_C Cuts at: 1936 4789 1936 Size: 2853 4594 SbfI CC_TGCA'GG Cuts at: 4799 4799 Size: 7447 ScaI AGT'ACT Cuts at: 2627 5695 7407 2627 Size: 3068 1712 2667 Fragments arranged by size: 3068 2667 1712 SexAI A'CCwGG_T Cuts at: 2385 3723 2385 Size: 1338 6109 SfcI C'TryA_G Cuts at: 826 1045 2443 3470 3827 3847 4550 4795 5930 Size: 219 1398 1027 357 20 703 245 1135 Cuts at: 5930 6611 6802 826 Size: 681 191 1471 Fragments arranged by size: 1471 1398 1135 1027 703 681 357 245 219 191 20 SfiI GGCCn_nnn'nGGCC Cuts at: 2030 2030 Size: 7447 SfoI GGC'GCC Cuts at: 2050 3285 2050 Size: 1235 6212 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7447 SmaI CCC'GGG Cuts at: 1872 4774 1872 Size: 2902 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2822 2844 3431 4724 5551 Size: 197 926 723 156 22 587 1293 827 Cuts at: 5551 6452 6714 820 Size: 901 262 1553 Fragments arranged by size: 1553 1293 926 901 827 723 587 262 197 156 22 SnaBI TAC'GTA Cuts at: 493 5238 493 Size: 4745 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7447 SphI G_CATG'C Cuts at: 1132 4805 1132 Size: 3673 3774 SspI AAT'ATT Cuts at: 5 52 5371 5 Size: 47 5319 2081 Fragments arranged by size: 5319 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7447 StyI C'CwwG_G Cuts at: 513 1065 2004 4500 4662 4734 4886 513 Size: 552 939 2496 162 72 152 3074 Fragments arranged by size: 3074 2496 939 552 162 152 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 2866 3323 4340 5474 5491 5644 Size: 1255 897 389 457 1017 1134 17 153 Cuts at: 5644 5829 6274 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1134 1017 897 457 445 389 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 4443 5693 Size: 274 80 33 51 1443 648 1818 1250 Cuts at: 5693 7405 96 Size: 1712 138 Fragments arranged by size: 1818 1712 1443 1250 648 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4049 4081 7407 7439 4049 Size: 32 3326 32 4057 Fragments arranged by size: 4057 3326 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4637 1316 Size: 3321 4126 XbaI T'CTAG_A Cuts at: 2525 3049 4783 2525 Size: 524 1734 5189 Fragments arranged by size: 5189 1734 524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4082 4306 4082 Size: 224 7223 XhoI C'TCGA_G Cuts at: 1943 2822 2844 1943 Size: 879 22 6546 Fragments arranged by size: 6546 879 22 XmaI C'CCGG_G Cuts at: 1870 4772 1870 Size: 2902 4545 XmnI GAAnn'nnTTC Cuts at: 3797 5576 3797 Size: 1779 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5255 276 Size: 53 83 186 936 3721 2468 Fragments arranged by size: 3721 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI SacII SanDI SapI SrfI SwaI