Restriction map for
pFN21AE9688
##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MluI MluNI MroI
MscI MslI MspA1I MspI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 54 GGATC GGATC(4/5)
AspHI 595 GTGCTC G[AT]GC[AT]^C
AvaII 447 GGACC G^G[AT]CC
BmyI 595 GTGCTC G[AGT]GC[ACT]^C
BsaJI 267 CCCTGG C^C[ACGT][ACGT]GG
BsgI 317 GTGCAG GTGCAG(16/14)
BsiHKAI 595 GTGCTC G[AT]GC[AT]^C
BsiYI 268 CCTGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 268 CCTGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 264 GTCCC GGGAC(10/14)
Bsp1286I 595 GTGCTC G[AGT]GC[ACT]^C
BspMI 326 ACCTGC ACCTGC(4/8)
Cac8I 319 GCAGGC GC[ACGT]^[ACGT]GC
DraI 801 TTTAAA TTT^AAA
HinfI 379 GATTC G^A[ACGT]TC
HpaI 367 GTTAAC GTT^AAC
HphI 599 TCACC GGTGA(8/7)
MfeI 603 CAATTG C^AATTG
MunI 603 CAATTG C^AATTG
NlaIV 852 GGTTCC GG[ACGT]^[ACGT]CC
SspI 790 AATATT AAT^ATT
TaqI 354 TCGA T^CGA
TfiI 379 GATTC G^A[AT]TC
TspRI 375 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 354 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 688 AAATTT [GA]^AATT[CT]
797 AAATTT
ApoI 688 AAATTT [GA]^AATT[CT]
797 AAATTT
BbvI 142 GCTGC GCAGC(8/12)
641 GCTGC
BfaI 74 CTAG C^TAG
664 CTAG
BglII 83 AGATCT A^GATCT
564 AGATCT
BpmI 372 CTCCAG CTGGAG(16/14)
513 CTGGAG
BssKI 268 CCTGG ^CC[ACGT]GG
856 CCAGG
BstNI 268 CCTGG CC^[AT]GG
856 CCAGG
BstYI 83 AGATCT [GA]^GATC[CT]
564 AGATCT
Cfr13I 447 GGACC G^G[ACGT]CC
462 GGCCC
EcoRII 268 CCTGG ^CC[AT]GG
856 CCAGG
Fnu4HI 142 GCTGC GC^[ACGT]GC
641 GCTGC
HaeIII 322 GGCC GG^CC
462 GGCC
HincII 367 GTTAAC GT[CT]^[GA]AC
649 GTTGAC
HindII 367 GTTAAC GT[CT]^[GA]AC
649 GTTGAC
ItaI 142 GCTGC GC^[ACGT]GC
641 GCTGC
MaeI 74 CTAG C^TAG
664 CTAG
MflI 83 AGATCT [GA]^GATC[CT]
564 AGATCT
MvaI 268 CCTGG CC^[AT]GG
856 CCAGG
Sau96I 447 GGACC G^G[ACGT]CC
462 GGCCC
ScrFI 268 CCTGG CC^[ACGT]GG
856 CCAGG
SfaNI 889 GATGC GCATC(5/9)
4 GCATC
TseI 142 GCTGC G^C[AT]GC
641 GCTGC
Tsp45I 284 GTGAC ^GT[GC]AC
672 GTCAC
XhoII 83 AGATCT [GA]^GATC[CT]
564 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BsrI BstF5I DdeI DpnII
FokI MaeIII MboI MboII MnlI
MseI NdeII NlaIII Sau3AI Tru9I
Tsp509I