Restriction map for
pFN21AE9688
(Circular) (Six-base) MAPSORT of: pFN21AE9688.seq Check: 6850 from: 1 to: 5617
pFN21AE9688 5617 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2340 2340
Size: 5617
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3680 5064 5332 1632
Size: 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2914 4516 5493 1203
Size: 1711 1602 977 1327
Fragments arranged by size:
1711 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5617
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2693 2802 2926 3152 3304 5413 1493
Size: 1200 109 124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1200 226 152 124 109
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2942 1870
Size: 73 999 4545
Fragments arranged by size:
4545 999 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 4393 618
Size: 325 217 627 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2936 1391
Size: 1545 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2088 2569 5612 2088
Size: 481 3043 2093
Fragments arranged by size:
3043 2093 481
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 3678 4834 1790
Size: 1888 1156 2573
Fragments arranged by size:
2573 1888 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2362 2539 4277 1239
Size: 1123 177 1738 2579
Fragments arranged by size:
2579 1738 1123 177
Bpu10I CC'TnA_GC
Cuts at: 2883 2883
Size: 5617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5392
Fragments arranged by size:
5392 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5617
BseYI C'CCAG_C
Cuts at: 4820 5316 4820
Size: 496 5121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2343 1163
Size: 573 607 4437
Fragments arranged by size:
4437 607 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2604 3678 3763 4834 2604
Size: 1074 85 1071 3387
Fragments arranged by size:
3387 1074 1071 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2604 2936 3678 3763 4834 1391
Size: 399 814 332 742 85 1071 2174
Fragments arranged by size:
2174 1071 814 742 399 332 85
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2972 844
Size: 1496 632 3489
Fragments arranged by size:
3489 1496 632
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 1394
Size: 429 1681 3507
Fragments arranged by size:
3507 1681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4261 4448 1700
Size: 110 2451 187 2869
Fragments arranged by size:
2869 2451 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2088 2569 2947 3314 3699 3716 5168 5254
Size: 116 481 378 367 385 17 1452 86
Cuts at: 5254 5266 5612 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 481 385 378 367 346 116
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 3133 3927 3947 3133
Size: 794 20 4803
Fragments arranged by size:
4803 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2808 2921 2808
Size: 113 5504
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3064 3953 5582
Size: 54 1425 50 275 18 1234 889 1629
Cuts at: 5582 8
Size: 43
Fragments arranged by size:
1629 1425 1234 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3547 1353
Size: 2194 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2539 3680 4277 5064 5332 1239
Size: 393 730 177 1141 597 787 268 1524
Fragments arranged by size:
1524 1141 787 730 597 393 268 177
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2554 2586 4258
Size: 32 135 32 472 32 478 32 1672
Cuts at: 4258 4290 4332 4364 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1672 478 472 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 2656 2961 3207 669
Size: 988 281 436 282 305 246 3079
Fragments arranged by size:
3079 988 436 305 282 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 2374 3207 2374
Size: 833 4784
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 2608 3216 2608
Size: 608 5009
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2149 4731 4915 1305
Size: 844 2582 184 2007
Fragments arranged by size:
2582 2007 844 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3575 3934 4093 518
Size: 648 2409 359 159 2042
Fragments arranged by size:
2409 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3033 3710 4858 5103 1606
Size: 1427 677 1148 245 2120
Fragments arranged by size:
2120 1427 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2918 2975 4520 1132
Size: 97 1689 57 1545 2229
Fragments arranged by size:
2229 1689 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 2914 4516 2914
Size: 1602 4015
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PfoI T'CCnGG_A
Cuts at: 2860 2860
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 2783 3187 2783
Size: 404 5213
PstI C_TGCA'G
Cuts at: 830 2969 830
Size: 2139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2965 4100 4781 4972 826
Size: 219 1920 1135 681 191 1471
Fragments arranged by size:
1920 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3721 4622 4884 820
Size: 197 926 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 2797 3541 5
Size: 47 2745 744 2081
Fragments arranged by size:
2745 2081 744 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 3056 513
Size: 552 939 1052 3074
Fragments arranged by size:
3074 1052 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3661 3814 3999 4444 325
Size: 1255 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 5577 5609 2163
Size: 32 3382 32 2171
Fragments arranged by size:
3382 2171 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI