################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 154 GCGG CCGC(-3/-1) AlwNI 282 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 666 GGTCC G^G[AT]CC BbsI 778 GTCTTC GAAGAC(2/6) BbvCI 303 CCTCAGC CCTCAGC(-5/-2) BbvI 627 GCAGC GCAGC(8/12) BglII 119 AGATCT A^GATCT BpmI 159 CTCCAG CTGGAG(16/14) BpuAI 778 GTCTTC GAAGAC(2/6) BsaAI 710 TACGTG [CT]AC^GT[GA] BsaI 156 GGTCTC GGTCTC(1/5) BseRI 366 GAGGAG GAGGAG(10/8) BsrDI 511 GCAATG GCAATG(2/0) BsrFI 271 ACCGGC [GA]^CCGG[CT] BstF5I 831 CATCC GGATG(2/0) BstXI 792 CCAAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 271 ACCGGC [GA]^CCGG[CT] DraII 402 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 96 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 133 GACCAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 633 CCATGG C^C[GA][CT]GG EaeI 273 CGGCCA [CT]^GGCC[GA] EarI 108 GAAGAG CTCTTC(1/4) EcoO109I 402 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 576 GATATC GAT^ATC Fnu4HI 627 GCAGC GC^[ACGT]GC FokI 831 CATCC GGATG(9/13) HapII 272 CCGG C^CGG HpaII 272 CCGG C^CGG HphI 105 GGTGA GGTGA(8/7) ItaI 627 GCAGC GC^[ACGT]GC Ksp632I 108 GAAGAG CTCTTC(1/4) MaeII 711 ACGT A^CGT MslI 384 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 807 CAGCTG C[AC]G^C[GT]G MspI 272 CCGG C^CGG NcoI 633 CCATGG C^CATGG NheI 543 GCTAGC G^CTAGC NlaIV 402 GGGGCC GG[ACGT]^[ACGT]CC PflMI 95 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 807 CAGCTG CAG^CTG SfaNI 624 GATGC GCATC(5/9) TseI 627 GCAGC G^C[AT]GC Tsp45I 142 GTCAC ^GT[GC]AC TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 95 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 29 CCATTCTAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 60 AAATTT [GA]^AATT[CT] 90 AAATTC AlwI 652 GATCC GGATC(4/5) 643 GGATC ApoI 60 AAATTT [GA]^AATT[CT] 90 AAATTC BsaJI 633 CCATGG C^C[ACGT][ACGT]GG 662 CCAAGG BsiYI 95 CCACCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCTGATTTGGG BslI 95 CCACCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCTGATTTGGG BsmAI 111 GAGAC GTCTC(1/5) 157 GTCTC BsrI 287 ACTGG ACTGG(1/-1) 656 CCAGT Cfr13I 403 GGGCC G^G[ACGT]CC 666 GGTCC DdeI 304 CTCAG C^T[ACGT]AG 552 CTTAG EcoT14I 633 CCATGG C^C[AT][AT]GG 662 CCAAGG MwoI 275 GCCATTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 396 GCTGTTGGGGC Sau96I 403 GGGCC G^G[ACGT]CC 666 GGTCC StyI 633 CCATGG C^C[AT][AT]GG 662 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AfaI AluI BfaI BstYI Cac8I DpnII HaeIII HinfI MaeI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII RsaI Sau3AI SmlI TfiI Tru9I Tsp509I XhoII