Restriction map for
pFN21AE9289
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI FauI FseI FspI
HaeII HaeIII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 663 GACGCC G[GA]^CG[CT]C
AflIII 377 ACATGT A^C[GA][CT]GT
AlwI 502 GGATC GGATC(4/5)
AlwNI 62 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 50 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 103 GAGCTC G[AT]GC[AT]^C
BclI 173 TGATCA T^GATCA
BsaHI 663 GACGCC G[GA]^CG[CT]C
BsiHKAI 103 GAGCTC G[AT]GC[AT]^C
BsmAI 531 GAGAC GTCTC(1/5)
Bsp1407I 431 TGTACA T^GTACA
BspLU11I 377 ACATGT A^CATGT
BsrDI 702 CATTGC GCAATG(2/0)
BsrFI 517 ACCGGC [GA]^CCGG[CT]
BsrGI 431 TGTACA T^GTACA
BsrI 630 ACTGG ACTGG(1/-1)
BssKI 846 CCAGG ^CC[ACGT]GG
BstNI 846 CCAGG CC^[AT]GG
BstXI 275 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 502 GGATCT [GA]^GATC[CT]
CfoI 114 GCGC GCG^C
Cfr10I 517 ACCGGC [GA]^CCGG[CT]
DsaI 839 CCATGG C^C[GA][CT]GG
EcoRII 846 CCAGG ^CC[AT]GG
EcoRV 247 GATATC GAT^ATC
EcoT22I 327 ATGCAT ATGCA^T
FbaI 173 TGATCA T^GATCA
HapII 518 CCGG C^CGG
HgaI 663 GACGC GACGC(5/10)
HhaI 114 GCGC GCG^C
Hin1I 663 GACGCC G[GA]^CG[CT]C
HinP1I 114 GCGC G^CGC
HpaII 518 CCGG C^CGG
MflI 502 GGATCT [GA]^GATC[CT]
MslI 150 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 109 CAGCTG C[AC]G^C[GT]G
MspI 518 CCGG C^CGG
MvaI 846 CCAGG CC^[AT]GG
NcoI 839 CCATGG C^CATGG
NdeI 395 CATATG CA^TATG
NsiI 327 ATGCAT ATGCA^T
NspI 377 ACATGT [GA]CATG^[CT]
PflMI 768 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 109 CAGCTG CAG^CTG
SacI 103 GAGCTC GAGCT^C
ScrFI 846 CCAGG CC^[ACGT]GG
SspBI 431 TGTACA T^GTACA
TfiI 137 GAATC G^A[AT]TC
Van91I 768 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 502 GGATCT [GA]^GATC[CT]
XmnI 50 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 402 CCGC CCGC(-3/-1)
717 CCGC
AvaII 740 GGTCC G^G[AT]CC
843 GGACC
BpmI 106 CTCCAG CTGGAG(16/14)
606 CTCCAG
Cac8I 666 GCCTGC GC[ACGT]^[ACGT]GC
706 GCCAGC
Cfr13I 740 GGTCC G^G[ACGT]CC
843 GGACC
EarI 118 CTCTTC CTCTTC(1/4)
580 CTCTTC
EcoT14I 498 CCTTGG C^C[AT][AT]GG
839 CCATGG
Ksp632I 118 CTCTTC CTCTTC(1/4)
580 CTCTTC
PleI 333 GACTC GAGTC(4/5)
533 GACTC
Sau96I 740 GGTCC G^G[ACGT]CC
843 GGACC
SfaNI 494 GCATC GCATC(5/9)
710 GCATC
SmlI 165 CTTGAG C^T[CT][GA]AG
642 CTTGAG
StyI 498 CCTTGG C^C[AT][AT]GG
839 CCATGG
Tsp509I 510 AATT ^AATT
626 AATT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmI Bsp1286I
BspMI BstF5I DdeI DpnII Fnu4HI
FokI HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII PstI RsaI Sau3AI
SfcI Tru9I TseI Tsp45I TspRI