################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BpuAI BsaBI BsaHI BsaI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AfaI 720 GTAC GT^AC Asp700I 918 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 805 GTGCTC G[AT]GC[AT]^C BalI 569 TGGCCA TGG^CCA BamHI 164 GGATCC G^GATCC BanI 877 GGCACC G^G[CT][GA]CC BclI 840 TGATCA T^GATCA BcnI 801 CCCGG CC^[GC]GG BglII 483 AGATCT A^GATCT BpmI 355 CTGGAG CTGGAG(16/14) BsaAI 604 CACGTA [CT]AC^GT[GA] BsaWI 40 ACCGGA [AT]^CCGG[AT] BseRI 325 GAGGAG GAGGAG(10/8) BsiEI 939 CGACCG CG[GA][CT]^CG BsiHKAI 805 GTGCTC G[AT]GC[AT]^C BsmAI 797 GTCTC GTCTC(1/5) BsmI 962 GAATGC GAATGC(1/-1) BspHI 759 TCATGA T^CATGA Bst1107I 372 GTATAC GTA^TAC BstNI 180 CCAGG CC^[AT]GG BstXI 83 CCAGCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 372 GTATAC GTA^TAC DraII 746 AGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 274 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 403 GACATCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 278 CCGTGG C^C[GA][CT]GG EaeI 569 TGGCCA [CT]^GGCC[GA] EarI 283 GAAGAG CTCTTC(1/4) EcoNI 768 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 746 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 180 CCAGG ^CC[AT]GG EcoT14I 320 CCAAGG C^C[AT][AT]GG FbaI 840 TGATCA T^GATCA HaeII 676 AGCGCC [GA]GCGC^[CT] HgaI 701 GCGTC GACGC(5/10) HinfI 505 GAGTC G^A[ACGT]TC Ksp632I 283 GAAGAG CTCTTC(1/4) MluNI 569 TGGCCA TGG^CCA MscI 569 TGGCCA TGG^CCA MseI 755 TTAA T^TAA MslI 681 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 51 CAGCGG C[AC]G^C[GT]G MvaI 180 CCAGG CC^[AT]GG NciI 801 CCCGG CC^[GC]GG NspI 170 GCATGC [GA]CATG^[CT] PflMI 273 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 505 GAGTC GAGTC(4/5) PpuMI 746 AGGTCCC [GA]G^G[AT]CC[CT] PstI 23 CTGCAG CTGCA^G RcaI 759 TCATGA T^CATGA RsaI 720 GTAC GT^AC SfcI 23 CTGCAG C^T[GA][CT]AG SphI 170 GCATGC GCATG^C SspI 308 AATATT AAT^ATT StyI 320 CCAAGG C^C[AT][AT]GG Tru9I 755 TTAA T^TAA Van91I 273 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 918 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 372 GTATAC GT^[AC][GT]AC 598 GTAGAC AccII 700 CGCG CG^CG 967 CGCG AvaII 38 GGACC G^G[AT]CC 747 GGTCC BlpI 550 GCTGAGC GC^T[ACGT]AGC 807 GCTCAGC Bpu1102I 550 GCTGAGC GC^T[ACGT]AGC 807 GCTCAGC BsgI 691 CTGCAC GTGCAG(16/14) 196 GTGCAG BspMI 198 GCAGGT ACCTGC(4/8) 420 GCAGGT BssKI 180 CCAGG ^CC[ACGT]GG 801 CCCGG BstUI 700 CGCG CG^CG 967 CGCG CelII 550 GCTGAGC GC^T[ACGT]AGC 807 GCTCAGC HapII 41 CCGG C^CGG 802 CCGG HpaII 41 CCGG C^CGG 802 CCGG MaeIII 103 GTGAC ^GT[ACGT]AC 791 GTCAC MspI 41 CCGG C^CGG 802 CCGG MvnI 700 CGCG CG^CG 967 CGCG ScrFI 180 CCAGG CC^[ACGT]GG 801 CCCGG StuI 728 AGGCCT AGG^CCT 776 AGGCCT TaqI 242 TCGA T^CGA 437 TCGA Tsp45I 103 GTGAC ^GT[GC]AC 791 GTCAC TthHB8I 242 TCGA T^CGA 437 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BstF5I BstYI Cac8I CfoI Cfr13I DdeI DpnII FauI Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MaeII MboI MboII MflI MnlI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I SfaNI TseI TspRI XhoII