(Circular) (Six-base) MAPSORT of: pFN21AE9284.seq Check: 1663 from: 1 to: 5686 pFN21AE9284 5686 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2194 2416 2194 Size: 222 5464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2378 2604 3029 1937 Size: 441 226 425 4594 Fragments arranged by size: 4594 441 425 226 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 2760 3749 5133 5401 1632 Size: 848 280 989 1384 268 1917 Fragments arranged by size: 1917 1384 989 848 280 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2984 1184 Size: 1800 3886 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 2321 Size: 32 5654 AlwNI CAG_nnn'CTG Cuts at: 3429 5001 3429 Size: 1572 4114 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 2169 3016 3383 2169 Size: 847 367 4472 Fragments arranged by size: 4472 847 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2882 3007 4462 618 Size: 325 217 627 1095 125 1455 1842 Fragments arranged by size: 1842 1455 1095 627 325 217 125 BanII G_rGCy'C Cuts at: 1391 2218 2450 2868 2979 3005 1391 Size: 827 232 418 111 26 4072 Fragments arranged by size: 4072 827 418 232 111 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 2845 1548 Size: 1297 4389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4297 136 Size: 107 122 71 631 605 337 2288 1525 Fragments arranged by size: 2288 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2488 5681 2488 Size: 3193 2493 BlpI GC'TnA_GC Cuts at: 2556 2813 3103 2556 Size: 257 290 5139 Fragments arranged by size: 5139 290 257 Bme1580I G_kGCm'C Cuts at: 1790 3747 4903 1790 Size: 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 BmgBI CAC'GTC Cuts at: 1318 2416 2860 1318 Size: 1098 444 4144 Fragments arranged by size: 4144 1098 444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2121 3417 4375 Size: 416 53 83 399 24 704 1296 958 Cuts at: 4375 442 Size: 1753 Fragments arranged by size: 1753 1296 958 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 4346 1239 Size: 1142 1965 2579 Fragments arranged by size: 2579 1965 1142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4349 882 Size: 617 2850 2219 Fragments arranged by size: 2850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2611 3477 493 Size: 711 1407 866 2702 Fragments arranged by size: 2702 1407 866 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3493 3875 4510 275 Size: 53 83 186 936 1960 382 635 1451 Fragments arranged by size: 1960 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2045 4119 4517 4791 4938 5349 2045 Size: 2074 398 274 147 411 2382 Fragments arranged by size: 2382 2074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2348 2351 2378 1178 Size: 30 165 30 918 27 3 27 4486 Fragments arranged by size: 4486 918 165 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2345 1186 Size: 1159 4527 BseYI C'CCAG_C Cuts at: 2087 4889 5385 2087 Size: 2802 496 2388 Fragments arranged by size: 2802 2388 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 2681 1163 Size: 573 486 459 4168 Fragments arranged by size: 4168 573 486 459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2947 3136 3897 4925 1540 Size: 275 186 946 189 761 1028 2301 Fragments arranged by size: 2301 1028 946 761 275 189 186 BsiHKAI G_wGCw'C Cuts at: 2814 3747 3832 4903 2814 Size: 933 85 1071 3597 Fragments arranged by size: 3597 1071 933 85 BsmI GAATG_Cn' Cuts at: 2973 3197 3290 2973 Size: 224 93 5369 Fragments arranged by size: 5369 224 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 2450 2814 2868 2979 3005 3747 Size: 399 428 232 364 54 111 26 742 Cuts at: 3747 3832 4903 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 428 399 364 232 111 85 54 26 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 2764 3575 5305 2764 Size: 811 1730 3145 Fragments arranged by size: 3145 1730 811 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2194 2416 3041 844 Size: 1350 222 625 3489 Fragments arranged by size: 3489 1350 625 222 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4181 4355 65 Size: 1183 2933 174 1396 Fragments arranged by size: 2933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstXI CCAn_nnnn'nTGG Cuts at: 2095 2095 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2169 2488 2769 3016 3383 3768 3785 5237 Size: 197 319 281 247 367 385 17 1452 Cuts at: 5237 5323 5335 5681 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 319 281 247 197 86 17 12 BstZ17I GTA'TAC Cuts at: 2379 2379 Size: 5686 BtgI C'CryG_G Cuts at: 513 1065 2004 2283 513 Size: 552 939 279 3916 Fragments arranged by size: 3916 939 552 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3202 3996 4016 2641 Size: 561 794 20 4311 Fragments arranged by size: 4311 794 561 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DraIII CAC_nnn'GTG Cuts at: 2284 2284 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2414 4693 809 Size: 507 1098 2279 1802 Fragments arranged by size: 2279 1802 1098 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 3133 4022 Size: 54 1425 50 275 18 744 559 889 Cuts at: 4022 5651 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 744 559 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 3616 1353 Size: 930 1333 3423 Fragments arranged by size: 3423 1333 930 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4273 4647 4793 387 Size: 1064 138 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2480 2760 3749 4346 5133 5401 Size: 393 749 99 280 989 597 787 268 Cuts at: 5401 1239 Size: 1524 Fragments arranged by size: 1524 989 787 749 597 393 280 268 99 EcoNI CCTnn'n_nnAGG Cuts at: 2777 2777 Size: 5686 EcoO109I rG'GnC_Cy Cuts at: 2752 2752 Size: 5686 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2734 2734 Size: 5686 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2685 4833 1678 Size: 1007 2148 2531 Fragments arranged by size: 2531 2148 1007 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2258 2290 2554 Size: 32 135 32 628 32 26 32 264 Cuts at: 2554 2586 2940 2972 4327 4359 4401 4433 1373 Size: 32 354 32 1355 32 42 32 2626 Fragments arranged by size: 2626 1355 628 354 264 135 42 32 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 10 Size: 54 1425 343 744 3120 Fragments arranged by size: 3120 1425 744 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3644 4003 4162 518 Size: 648 1524 954 359 159 2042 Fragments arranged by size: 2042 1524 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 3102 3779 4927 5172 1606 Size: 452 1044 677 1148 245 2120 Fragments arranged by size: 2120 1148 1044 677 452 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4195 Fragments arranged by size: 4195 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2179 3044 4589 1132 Size: 97 950 865 1545 2229 Fragments arranged by size: 2229 1545 950 865 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 1108 Size: 1176 4510 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2353 3755 3787 5268 5300 2321 Size: 32 1402 32 1481 32 2707 Fragments arranged by size: 2707 1481 1402 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2752 2752 Size: 5686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 2032 3038 830 Size: 1202 1006 3478 Fragments arranged by size: 3478 1202 1006 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2028 3034 4169 4850 5041 826 Size: 219 983 1006 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 983 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 2179 3044 1132 Size: 1047 865 3774 Fragments arranged by size: 3774 1047 865 SspI AAT'ATT Cuts at: 5 52 2315 3610 5 Size: 47 2263 1295 2081 Fragments arranged by size: 2263 2081 1295 47 StuI AGG'CCT Cuts at: 1517 2735 2783 1517 Size: 1218 48 4420 Fragments arranged by size: 4420 1218 48 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 3125 513 Size: 552 939 321 800 3074 Fragments arranged by size: 3074 939 800 552 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2961 3713 3730 3883 4068 4513 325 Size: 1255 1381 752 17 153 185 445 1498 Fragments arranged by size: 1498 1381 1255 752 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2476 2508 5646 5678 2476 Size: 32 3138 32 2484 Fragments arranged by size: 3138 2484 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 2927 3815 2927 Size: 888 4798 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI