Restriction map for
pFN21AE9392
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI BamHI BbeI
BcgI BciVI BclI BcnI BfrI
BglI BglII BsiEI BsiWI Bsp1407I
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FbaI
FseI HpaI KasI KpnI KspI
MluI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 3027 GACGTC GACGT^C
AccI 580 GTCTAC GT^[AC][GT]AC
AccII 3244 CGCG CG^CG
AccIII 3074 TCCGGA T^CCGGA
AcyI 3027 GACGTC G[GA]^CG[CT]C
Asp700I 682 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1758 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3812 TGCGCA TGC^GCA
BbrPI 467 CACGTG CAC^GTG
BbvCI 2166 CCTCAGC CCTCAGC(-5/-2)
BlpI 1185 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1185 GCTGAGC GC^T[ACGT]AGC
BsaAI 467 CACGTG [CT]AC^GT[GA]
BsaBI 3383 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3027 GACGTC G[GA]^CG[CT]C
BseAI 3074 TCCGGA T^CCGGA
BsgI 974 GTGCAG GTGCAG(16/14)
BsmBI 3555 CGTCTC CGTCTC(1/5)
BspEI 3074 TCCGGA T^CCGGA
BsrBI 91 GAGCGG CCGCTC(-3/-3)
BssSI 1439 CTCGTG CACGAG(-5/-1)
BstUI 3244 CGCG CG^CG
BstXI 504 CCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1185 GCTGAGC GC^T[ACGT]AGC
DrdI 571 GACTTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2511 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2802 GATATC GAT^ATC
FspI 3812 TGCGCA TGC^GCA
HgaI 2409 GCGTC GACGC(5/10)
Hin1I 3027 GACGTC G[GA]^CG[CT]C
HindIII 324 AAGCTT A^AGCTT
MamI 3383 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3074 TCCGGA T^CCGGA
MvnI 3244 CGCG CG^CG
NheI 3363 GCTAGC G^CTAGC
NspI 3125 ACATGC [GA]CATG^[CT]
PflFI 1758 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 467 CACGTG CAC^GTG
PmlI 467 CACGTG CAC^GTG
PshAI 2415 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1328 CTGCAG CTGCA^G
SapI 2221 GAAGAGC GCTCTTC(1/4)
SfcI 1328 CTGCAG C^T[GA][CT]AG
SpeI 1750 ACTAGT A^CTAGT
StuI 1657 AGGCCT AGG^CCT
Tth111I 1758 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2332 TCTAGA T^CTAGA
XmnI 682 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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Alw44I 68 GTGCAC G^TGCAC
1236 GTGCAC
AocI 2446 CCTAAGG CC^T[ACGT]AGG
3300 CCTCAGG
ApaLI 68 GTGCAC G^TGCAC
1236 GTGCAC
AvaI 1975 CCCGAG C^[CT]CG[GA]G
2021 CTCGGG
AvrII 1279 CCTAGG C^CTAGG
1939 CCTAGG
BanII 1249 GAGCTC G[GA]GC[CT]^C
3295 GAGCTC
BlnI 1279 CCTAGG C^CTAGG
1939 CCTAGG
BsmI 1623 GAATGC GAATGC(1/-1)
3614 GCATTC
BsoBI 1975 CCCGAG C^[CT]CG[GA]G
2021 CTCGGG
BspDI 1161 ATCGAT AT^CGAT
3817 ATCGAT
BspHI 1890 TCATGA T^CATGA
2283 TCATGA
BspMI 1326 ACCTGC ACCTGC(4/8)
2367 ACCTGC
BstAPI 2362 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2836 GCATCCTCTGC
BstYI 3 GGATCT [GA]^GATC[CT]
2489 AGATCC
Bsu36I 2446 CCTAAGG CC^T[ACGT]AGG
3300 CCTCAGG
ClaI 1161 ATCGAT AT^CGAT
3817 ATCGAT
Eco81I 2446 CCTAAGG CC^T[ACGT]AGG
3300 CCTCAGG
FauI 1071 GCGGG CCCGC(4/6)
1343 GCGGG
HaeII 729 AGCGCC [GA]GCGC^[CT]
2507 AGCGCC
HincII 1743 GTCAAC GT[CT]^[GA]AC
3640 GTCAAC
HindII 1743 GTCAAC GT[CT]^[GA]AC
3640 GTCAAC
MfeI 911 CAATTG C^AATTG
1775 CAATTG
MflI 3 GGATCT [GA]^GATC[CT]
2489 AGATCC
MunI 911 CAATTG C^AATTG
1775 CAATTG
PflMI 1475 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1590 CCAACCAATGG
PpuMI 1634 AGGTCCC [GA]G^G[AT]CC[CT]
2666 AGGACCC
RcaI 1890 TCATGA T^CATGA
2283 TCATGA
SacI 1249 GAGCTC GAGCT^C
3295 GAGCTC
ScaI 3369 AGTACT AGT^ACT
3440 AGTACT
SspI 1846 AATATT AAT^ATT
3461 AATATT
Van91I 1475 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1590 CCAACCAATGG
XhoII 3 GGATCT [GA]^GATC[CT]
2489 AGATCC
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI