(Circular) (Six-base) MAPSORT of: pFN21AE9392.seq Check: 1531 from: 1 to: 8539 pFN21AE9392 8539 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3340 4381 3340 Size: 1041 7498 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5036 6349 278 Size: 53 83 186 936 3500 1313 2468 Fragments arranged by size: 3500 2468 1313 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2586 5882 1937 Size: 649 3296 4594 Fragments arranged by size: 4594 3296 649 Acc65I G'GTAC_C Cuts at: 5860 5860 Size: 8539 AclI AA'CG_TT Cuts at: 1563 6666 7039 1563 Size: 5103 373 3063 Fragments arranged by size: 5103 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2373 3206 4797 5171 5345 5444 6602 Size: 492 249 833 1591 374 174 99 1158 Cuts at: 6602 7986 8254 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1591 1384 1158 833 492 374 268 249 174 99 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8342 AflIII A'CryG_T Cuts at: 1203 7438 8415 1203 Size: 6235 977 1327 Fragments arranged by size: 6235 1327 977 AgeI A'CCGG_T Cuts at: 7370 7370 Size: 8539 AhdI GACnn_n'nnGTC Cuts at: 7268 7268 Size: 8539 AleI CACnn'nnGTG Cuts at: 1166 4433 1166 Size: 3267 5272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4031 5395 1184 Size: 2847 1364 4328 Fragments arranged by size: 4328 2847 1364 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5522 5554 5522 Size: 32 8507 AlwNI CAG_nnn'CTG Cuts at: 2031 3091 3404 3464 4373 4516 4931 6282 7854 Size: 1060 313 60 909 143 415 1351 1572 Cuts at: 7854 2031 Size: 2716 Fragments arranged by size: 2716 1572 1351 1060 909 415 313 143 60 ApaLI G'TGCA_C Cuts at: 2073 3241 6596 7752 2073 Size: 1168 3355 1156 2860 Fragments arranged by size: 3355 2860 1168 1156 ApoI r'AATT_y Cuts at: 1493 2239 2280 2290 2425 2720 2921 3714 4031 Size: 746 41 10 135 295 201 793 317 Cuts at: 4031 4784 5180 5720 5848 6074 6226 8335 1493 Size: 753 396 540 128 226 152 2109 1697 Fragments arranged by size: 2109 1697 793 753 746 540 396 317 295 226 201 152 135 128 41 10 AseI AT'TA_AT Cuts at: 160 1032 7093 160 Size: 872 6061 1606 Fragments arranged by size: 6061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8539 AvaI C'yCGr_G Cuts at: 1870 1943 3980 4026 5864 1870 Size: 73 2037 46 1838 4545 Fragments arranged by size: 4545 2037 1838 73 46 AvrII C'CTAG_G Cuts at: 3284 3944 3284 Size: 660 7879 BamHI G'GATC_C Cuts at: 5869 6236 5869 Size: 367 8172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2884 3439 4157 5860 7315 Size: 325 217 627 1097 555 718 1703 1455 Cuts at: 7315 618 Size: 1842 Fragments arranged by size: 1842 1703 1455 1097 718 627 555 325 217 BanII G_rGCy'C Cuts at: 1391 3258 5304 5858 1391 Size: 1867 2046 554 4072 Fragments arranged by size: 4072 2046 1867 554 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2580 2652 3230 3629 928 Size: 971 231 450 72 578 399 5838 Fragments arranged by size: 5838 971 578 450 399 231 72 BbvCI CC'TCA_GC Cuts at: 4172 4172 Size: 8539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6730 6764 1903 Size: 34 4793 34 3678 Fragments arranged by size: 4793 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6431 7641 6431 Size: 1210 7329 BclI T'GATC_A Cuts at: 1548 1548 Size: 8539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7150 136 Size: 107 122 71 631 605 5478 1525 Fragments arranged by size: 5478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8534 8534 Size: 8539 BlpI GC'TnA_GC Cuts at: 3191 5956 3191 Size: 2765 5774 Bme1580I G_kGCm'C Cuts at: 1790 2077 2301 3245 6600 7756 1790 Size: 287 224 944 3355 1156 2573 Fragments arranged by size: 3355 2573 1156 944 287 224 BmgBI CAC'GTC Cuts at: 1318 5644 1318 Size: 4326 4213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 2970 3039 Size: 416 53 83 399 24 624 929 69 Cuts at: 3039 5537 5594 5692 6270 7228 442 Size: 2498 57 98 578 958 1753 Fragments arranged by size: 2498 1753 958 929 624 578 416 399 98 83 69 57 53 24 BmtI G_CTAG'C Cuts at: 1056 5372 1056 Size: 4316 4223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3400 3490 3400 Size: 90 8449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3349 3441 4143 4538 5045 5434 7199 1239 Size: 2110 92 702 395 507 389 1765 2579 Fragments arranged by size: 2579 2110 1765 702 507 395 389 92 Bpu10I CC'TnA_GC Cuts at: 3465 4172 3465 Size: 707 7832 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2544 3194 3233 6664 7529 7827 2544 Size: 650 39 3431 865 298 3256 Fragments arranged by size: 3431 3256 865 650 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2748 4076 5077 7202 882 Size: 617 1249 1328 1001 2125 2219 Fragments arranged by size: 2219 2125 1328 1249 1001 617 BsaAI yAC'GTr Cuts at: 493 1204 2474 6330 493 Size: 711 1270 3856 2702 Fragments arranged by size: 3856 2702 1270 711 BsaBI GATnn'nnATC Cuts at: 5392 8533 5392 Size: 3141 5398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5033 6346 6728 7363 Size: 53 83 186 936 3500 1313 382 635 Cuts at: 7363 275 Size: 1451 Fragments arranged by size: 3500 1451 1313 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2707 2887 5079 6972 7370 7644 7791 8202 2707 Size: 180 2192 1893 398 274 147 411 3044 Fragments arranged by size: 3044 2192 1893 411 398 274 180 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 5018 5048 5546 Size: 30 165 30 630 30 2955 30 498 Cuts at: 5546 5576 1178 Size: 30 4141 Fragments arranged by size: 4141 2955 630 498 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3573 3659 4535 4638 5294 5390 1186 Size: 2387 86 876 103 656 96 4335 Fragments arranged by size: 4335 2387 876 656 103 96 86 BseYI C'CCAG_C Cuts at: 2590 3321 3643 5613 5760 7742 8238 2590 Size: 731 322 1970 147 1982 496 2891 Fragments arranged by size: 2891 1982 1970 731 496 322 147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3000 1163 Size: 573 1264 6702 Fragments arranged by size: 6702 1264 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5989 6750 7778 1540 Size: 275 186 3988 761 1028 2301 Fragments arranged by size: 3988 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2077 3245 3258 5304 6600 6685 7756 2077 Size: 1168 13 2046 1296 85 1071 2860 Fragments arranged by size: 2860 2046 1296 1168 1071 85 13 BsmI GAATG_Cn' Cuts at: 3634 5619 6050 6143 3634 Size: 1985 431 93 6030 Fragments arranged by size: 6030 1985 431 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5566 5566 Size: 8539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2301 3245 3258 5304 5858 6600 Size: 399 287 224 944 13 2046 554 742 Cuts at: 6600 6685 7756 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 2046 1071 944 742 554 399 287 224 85 13 BspEI T'CCGG_A Cuts at: 5079 8202 5079 Size: 3123 5416 BspHI T'CATG_A Cuts at: 3895 4288 6428 8158 3895 Size: 393 2140 1730 4276 Fragments arranged by size: 4276 2140 1730 393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3340 4381 5894 844 Size: 2496 1041 1513 3489 Fragments arranged by size: 3489 2496 1513 1041 BsrBI CCG'CTC Cuts at: 1394 1823 2098 6426 1394 Size: 429 275 4328 3507 Fragments arranged by size: 4328 3507 429 275 BsrDI GCAATG_nn' Cuts at: 65 1248 5242 5322 5527 5583 5665 7034 7208 Size: 1183 3994 80 205 56 82 1369 174 Cuts at: 7208 65 Size: 1396 Fragments arranged by size: 3994 1396 1369 1183 205 174 82 80 56 BsrFI r'CCGG_y Cuts at: 1700 1810 7183 7370 1700 Size: 110 5373 187 2869 Fragments arranged by size: 5373 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8539 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8539 BssSI C'ACGA_G Cuts at: 3444 6599 7611 3444 Size: 3155 1012 4372 Fragments arranged by size: 4372 3155 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4373 4847 794 Size: 3579 474 4486 Fragments arranged by size: 4486 3579 474 BstBI TT'CG_AA Cuts at: 7343 7343 Size: 8539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8539 BstXI CCAn_nnnn'nTGG Cuts at: 2516 2516 Size: 8539 BstYI r'GATC_y Cuts at: 1972 2008 4494 5869 6236 6621 6638 8090 8176 Size: 36 2486 1375 367 385 17 1452 86 Cuts at: 8176 8188 8534 1972 Size: 12 346 1977 Fragments arranged by size: 2486 1977 1452 1375 385 367 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 4452 5306 4452 Size: 854 7685 BtgI C'CryG_G Cuts at: 513 1065 2004 2865 3561 3698 4941 5241 5510 Size: 552 939 861 696 137 1243 300 269 Cuts at: 5510 513 Size: 3542 Fragments arranged by size: 3542 1243 939 861 696 552 300 269 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4717 487 Size: 683 56 3491 4309 Fragments arranged by size: 4309 3491 683 56 BtsI GCAGTG_nn' Cuts at: 2054 2785 3202 3243 3342 3924 4198 4661 4881 Size: 731 417 41 99 582 274 463 220 Cuts at: 4881 4893 6055 6849 6869 2054 Size: 12 1162 794 20 3724 Fragments arranged by size: 3724 1162 794 731 582 463 417 274 220 99 41 20 12 ClaI AT'CG_AT Cuts at: 1554 3167 5823 1554 Size: 1613 2656 4270 Fragments arranged by size: 4270 2656 1613 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8504 DraI TTT'AAA Cuts at: 5843 5843 Size: 8539 DraIII CAC_nnn'GTG Cuts at: 2275 3240 3446 2275 Size: 965 206 7368 Fragments arranged by size: 7368 965 206 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2582 7546 809 Size: 507 1266 4964 1802 Fragments arranged by size: 4964 1802 1266 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2872 3312 3544 Size: 54 1425 50 275 18 1042 440 232 Cuts at: 3544 4348 4944 5691 5986 6875 8504 8 Size: 804 596 747 295 889 1629 43 Fragments arranged by size: 1629 1425 1042 889 804 747 596 440 295 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5986 1537 Size: 275 4174 4090 Fragments arranged by size: 4174 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2616 4074 4221 6469 1353 Size: 1263 1458 147 2248 3423 Fragments arranged by size: 3423 2248 1458 1263 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7126 7500 7646 387 Size: 1064 138 5537 374 146 1280 Fragments arranged by size: 5537 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3256 5302 3256 Size: 2046 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2373 3206 3349 3441 4143 4538 Size: 393 492 249 833 143 92 702 395 Cuts at: 4538 4797 5045 5171 5345 5434 5444 6602 7199 Size: 259 248 126 174 89 10 1158 597 Cuts at: 7199 7986 8254 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1158 833 787 702 597 492 395 393 268 259 249 248 174 143 126 92 89 10 EcoNI CCTnn'n_nnAGG Cuts at: 4520 4520 Size: 8539 EcoO109I rG'GnC_Cy Cuts at: 3640 4001 4672 5750 3640 Size: 361 671 1078 6429 Fragments arranged by size: 6429 1078 671 361 EcoRI G'AATT_C Cuts at: 2280 2290 2425 2720 4031 5720 5848 6226 2280 Size: 10 135 295 1311 1689 128 378 4593 Fragments arranged by size: 4593 1689 1311 378 295 135 128 10 EcoRV GAT'ATC Cuts at: 4809 4809 Size: 8539 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3056 3227 3056 Size: 171 8368 FspI TGC'GCA Cuts at: 5819 7045 5819 Size: 1226 7313 HaeII r_GCGC'y Cuts at: 1678 2738 4516 7686 1678 Size: 1060 1778 3170 2531 Fragments arranged by size: 3170 2531 1778 1060 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3413 3445 3559 3591 3752 Size: 32 135 32 1841 32 114 32 161 Cuts at: 3752 3754 3784 3786 3951 3983 4319 4351 5018 Size: 2 30 2 165 32 336 32 667 Cuts at: 5018 5050 5076 5108 5491 5523 5737 5769 7180 Size: 32 26 32 383 32 214 32 1411 Cuts at: 7180 7212 7254 7286 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1841 1411 667 383 336 214 165 161 135 114 42 32 32 32 32 32 32 32 32 32 32 32 32 30 26 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3750 5647 5883 6129 669 Size: 988 281 1812 1897 236 246 3079 Fragments arranged by size: 3079 1897 1812 988 281 246 236 HindIII A'AGCT_T Cuts at: 748 2329 748 Size: 1581 6958 HpaI GTT'AAC Cuts at: 6129 6129 Size: 8539 KpnI G_GTAC'C Cuts at: 5864 5864 Size: 8539 MfeI C'AATT_G Cuts at: 2916 3780 6138 2916 Size: 864 2358 5317 Fragments arranged by size: 5317 2358 864 MluI A'CGCG_T Cuts at: 8415 8415 Size: 8539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3372 3619 4089 7653 7837 1305 Size: 2067 247 470 3564 184 2007 Fragments arranged by size: 3564 2067 2007 470 247 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2874 3314 3546 4350 10 Size: 54 1425 343 1042 440 232 804 4199 Fragments arranged by size: 4199 1425 1042 804 440 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 4181 4433 4736 5744 6497 6856 7015 Size: 648 3015 252 303 1008 753 359 159 Cuts at: 7015 518 Size: 2042 Fragments arranged by size: 3015 2042 1008 753 648 359 303 252 159 MspA1I CmG'CkG Cuts at: 1606 2594 3348 3647 4424 4433 5955 6632 7780 Size: 988 754 299 777 9 1522 677 1148 Cuts at: 7780 8025 1606 Size: 245 2120 Fragments arranged by size: 2120 1522 1148 988 777 754 677 299 245 9 NcoI C'CATG_G Cuts at: 513 1065 2004 2865 3561 5241 5510 513 Size: 552 939 861 696 1680 269 3542 Fragments arranged by size: 3542 1680 939 861 696 552 269 NdeI CA'TA_TG Cuts at: 387 387 Size: 8539 NheI G'CTAG_C Cuts at: 1052 5368 1052 Size: 4316 4223 NotI GC'GGCC_GC Cuts at: 5986 5986 Size: 8539 NspI r_CATG'y Cuts at: 1132 1229 5134 5897 7442 1132 Size: 97 3905 763 1545 2229 Fragments arranged by size: 3905 2229 1545 763 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8539 PciI A'CATG_T Cuts at: 7438 7438 Size: 8539 PflMI CCAn_nnn'nTGG Cuts at: 1108 3486 3601 5836 1108 Size: 2378 115 2235 3811 Fragments arranged by size: 3811 2378 2235 115 PfoI T'CCnGG_A Cuts at: 4607 4607 Size: 8539 PmeI GTTT'AAAC Cuts at: 5843 5843 Size: 8539 PmlI CAC'GTG Cuts at: 2474 2474 Size: 8539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6608 6640 8121 8153 6608 Size: 32 1481 32 6994 Fragments arranged by size: 6994 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3640 4672 3640 Size: 1032 7507 PshAI GACnn'nnGTC Cuts at: 1613 4424 1613 Size: 2811 5728 PsiI TTA'TAA Cuts at: 6109 6109 Size: 8539 PstI C_TGCA'G Cuts at: 830 3337 5891 830 Size: 2507 2554 3478 Fragments arranged by size: 3478 2554 2507 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8539 PvuII CAG'CTG Cuts at: 2594 3647 4424 4433 2594 Size: 1053 777 9 6700 Fragments arranged by size: 6700 1053 777 9 SacI G_AGCT'C Cuts at: 3258 5304 3258 Size: 2046 6493 SalI G'TCGA_C Cuts at: 1936 5881 1936 Size: 3945 4594 SapI GCTCTTCn'nnn_ Cuts at: 4221 4221 Size: 8539 SbfI CC_TGCA'GG Cuts at: 5891 5891 Size: 8539 ScaI AGT'ACT Cuts at: 5376 5447 6787 8499 5376 Size: 71 1340 1712 5416 Fragments arranged by size: 5416 1712 1340 71 SfcI C'TryA_G Cuts at: 826 1045 3333 5887 7022 7703 7894 826 Size: 219 2288 2554 1135 681 191 1471 Fragments arranged by size: 2554 2288 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8539 SmaI CCC'GGG Cuts at: 1872 5866 1872 Size: 3994 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2559 3173 3212 6643 7544 7806 Size: 197 926 616 614 39 3431 901 262 Cuts at: 7806 820 Size: 1553 Fragments arranged by size: 3431 1553 926 901 616 614 262 197 39 SnaBI TAC'GTA Cuts at: 493 6330 493 Size: 5837 2702 SpeI A'CTAG_T Cuts at: 152 3755 152 Size: 3603 4936 SphI G_CATG'C Cuts at: 1132 5897 1132 Size: 4765 3774 SspI AAT'ATT Cuts at: 5 52 3853 5468 6463 5 Size: 47 3801 1615 995 2081 Fragments arranged by size: 3801 2081 1615 995 47 StuI AGG'CCT Cuts at: 1517 3664 1517 Size: 2147 6392 StyI C'CwwG_G Cuts at: 513 1065 2004 2865 3284 3309 3561 3944 4143 Size: 552 939 861 419 25 252 383 199 Cuts at: 4143 5241 5343 5510 5800 5978 513 Size: 1098 102 167 290 178 3074 Fragments arranged by size: 3074 1098 939 861 552 419 383 290 252 199 178 167 102 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3574 4300 6566 6583 6736 6921 7366 Size: 1255 1994 726 2266 17 153 185 445 Cuts at: 7366 325 Size: 1498 Fragments arranged by size: 2266 1994 1498 1255 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2497 3993 5283 Size: 274 80 33 51 1443 520 1496 1290 Cuts at: 5283 5374 5445 6785 8497 96 Size: 91 71 1340 1712 138 Fragments arranged by size: 1712 1496 1443 1340 1290 520 274 138 91 80 71 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 8499 8531 2033 Size: 32 6434 32 2041 Fragments arranged by size: 6434 2041 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3766 1316 Size: 2450 6089 XbaI T'CTAG_A Cuts at: 4337 5875 4337 Size: 1538 7001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2213 5458 5575 2213 Size: 3245 117 5177 Fragments arranged by size: 5177 3245 117 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8539 XmaI C'CCGG_G Cuts at: 1870 5864 1870 Size: 3994 4545 XmnI GAAnn'nnTTC Cuts at: 2691 6668 2691 Size: 3977 4562 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5034 6347 276 Size: 53 83 186 936 3500 1313 2468 Fragments arranged by size: 3500 2468 1313 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI