Restriction map for
pFN21AE9392
(Linear) (Six-base) MAPSORT of: KIEE9392.seq Check: 857 from: 1 to: 3834
KIEE9392.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1335 2376 3834
Size: 1335 1041 1458
Fragments arranged by size:
1458 1335 1041
AatII G_ACGT'C
Cuts at: 0 3031 3834
Size: 3031 803
AccI GT'mk_AC
Cuts at: 0 581 3834
Size: 581 3253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 368 1201 2792 3166 3340 3439 3834
Size: 119 249 833 1591 374 174 99 395
Fragments arranged by size:
1591 833 395 374 249 174 119 99
AleI CACnn'nnGTG
Cuts at: 0 2428 3834
Size: 2428 1406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2026 3390 3834
Size: 2026 1364 444
Fragments arranged by size:
2026 1364 444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3517 3549 3834
Size: 3517 32 285
Fragments arranged by size:
3517 285 32
AlwNI CAG_nnn'CTG
Cuts at: 0 26 1086 1399 1459 2368 2511 2926 3834
Size: 26 1060 313 60 909 143 415 908
Fragments arranged by size:
1060 909 908 415 313 143 60 26
ApaLI G'TGCA_C
Cuts at: 0 68 1236 3834
Size: 68 1168 2598
Fragments arranged by size:
2598 1168 68
ApoI r'AATT_y
Cuts at: 0 234 275 285 420 715 916 1709 2026
Size: 234 41 10 135 295 201 793 317
Cuts at: 2026 2779 3175 3715 3834
Size: 753 396 540 119
Fragments arranged by size:
793 753 540 396 317 295 234 201
135 119 41 10
AvaI C'yCGr_G
Cuts at: 0 1975 2021 3834
Size: 1975 46 1813
Fragments arranged by size:
1975 1813 46
AvrII C'CTAG_G
Cuts at: 0 1279 1939 3834
Size: 1279 660 1895
Fragments arranged by size:
1895 1279 660
BanI G'GyrC_C
Cuts at: 0 879 1434 2152 3834
Size: 879 555 718 1682
Fragments arranged by size:
1682 879 718 555
BanII G_rGCy'C
Cuts at: 0 1253 3299 3834
Size: 1253 2046 535
Fragments arranged by size:
2046 1253 535
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 575 647 1225 1624 3834
Size: 125 450 72 578 399 2210
Fragments arranged by size:
2210 578 450 399 125 72
BbvCI CC'TCA_GC
Cuts at: 0 2167 3834
Size: 2167 1667
BlpI GC'TnA_GC
Cuts at: 0 1186 3834
Size: 1186 2648
Bme1580I G_kGCm'C
Cuts at: 0 72 296 1240 3834
Size: 72 224 944 2594
Fragments arranged by size:
2594 944 224 72
BmgBI CAC'GTC
Cuts at: 0 3639 3834
Size: 3639 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 965 1034 3532 3589 3687 3834
Size: 36 929 69 2498 57 98 147
Fragments arranged by size:
2498 929 147 98 69 57 36
BmtI G_CTAG'C
Cuts at: 0 3367 3834
Size: 3367 467
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1395 1485 3834
Size: 1395 90 2349
Fragments arranged by size:
2349 1395 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1344 1436 2138 2533 3040 3429 3834
Size: 1344 92 702 395 507 389 405
Fragments arranged by size:
1344 702 507 405 395 389 92
Bpu10I CC'TnA_GC
Cuts at: 0 1460 2167 3834
Size: 1460 707 1667
Fragments arranged by size:
1667 1460 707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1189 1228 3834
Size: 539 650 39 2606
Fragments arranged by size:
2606 650 539 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 743 2071 3072 3834
Size: 743 1328 1001 762
Fragments arranged by size:
1328 1001 762 743
BsaAI yAC'GTr
Cuts at: 0 469 3834
Size: 469 3365
BsaBI GATnn'nnATC
Cuts at: 0 3387 3834
Size: 3387 447
BsaHI Gr'CG_yC
Cuts at: 0 3028 3834
Size: 3028 806
BsaWI w'CCGG_w
Cuts at: 0 702 882 3074 3834
Size: 702 180 2192 760
Fragments arranged by size:
2192 760 702 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 3013 3043 3541 3571 3834
Size: 28 30 2955 30 498 30 263
Fragments arranged by size:
2955 498 263 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1568 1654 2530 2633 3289 3385 3834
Size: 1568 86 876 103 656 96 449
Fragments arranged by size:
1568 876 656 449 103 96 86
BseYI C'CCAG_C
Cuts at: 0 585 1316 1638 3608 3755 3834
Size: 585 731 322 1970 147 79
Fragments arranged by size:
1970 731 585 322 147 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 3834
Size: 995 2839
BsiHKAI G_wGCw'C
Cuts at: 0 72 1240 1253 3299 3834
Size: 72 1168 13 2046 535
Fragments arranged by size:
2046 1168 535 72 13
BsmI GAATG_Cn'
Cuts at: 0 1629 3614 3834
Size: 1629 1985 220
Fragments arranged by size:
1985 1629 220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3561 3834
Size: 3561 273
Bsp1286I G_dGCh'C
Cuts at: 0 72 296 1240 1253 3299 3834
Size: 72 224 944 13 2046 535
Fragments arranged by size:
2046 944 535 224 72 13
BspEI T'CCGG_A
Cuts at: 0 3074 3834
Size: 3074 760
BspHI T'CATG_A
Cuts at: 0 1890 2283 3834
Size: 1890 393 1551
Fragments arranged by size:
1890 1551 393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1335 2376 3834
Size: 1335 1041 1458
Fragments arranged by size:
1458 1335 1041
BsrBI CCG'CTC
Cuts at: 0 93 3834
Size: 93 3741
BsrDI GCAATG_nn'
Cuts at: 0 3237 3317 3522 3578 3660 3834
Size: 3237 80 205 56 82 174
Fragments arranged by size:
3237 205 174 82 80 56
BssSI C'ACGA_G
Cuts at: 0 1439 3834
Size: 1439 2395
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2368 2842 3834
Size: 2368 474 992
Fragments arranged by size:
2368 992 474
BstXI CCAn_nnnn'nTGG
Cuts at: 0 511 3834
Size: 511 3323
BstYI r'GATC_y
Cuts at: 0 3 2489 3834
Size: 3 2486 1345
Fragments arranged by size:
2486 1345 3
Bsu36I CC'TnA_GG
Cuts at: 0 2447 3301 3834
Size: 2447 854 533
Fragments arranged by size:
2447 854 533
BtgI C'CryG_G
Cuts at: 0 860 1556 1693 2936 3236 3505 3834
Size: 860 696 137 1243 300 269 329
Fragments arranged by size:
1243 860 696 329 300 269 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2712 3834
Size: 2712 1122
BtsI GCAGTG_nn'
Cuts at: 0 49 780 1197 1238 1337 1919 2193 2656
Size: 49 731 417 41 99 582 274 463
Cuts at: 2656 2876 2888 3834
Size: 220 12 946
Fragments arranged by size:
946 731 582 463 417 274 220 99
49 41 12
ClaI AT'CG_AT
Cuts at: 0 1162 3818 3834
Size: 1162 2656 16
Fragments arranged by size:
2656 1162 16
DraIII CAC_nnn'GTG
Cuts at: 0 270 1235 1441 3834
Size: 270 965 206 2393
Fragments arranged by size:
2393 965 270 206
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 3834
Size: 577 3257
EaeI y'GGCC_r
Cuts at: 0 867 1307 1539 2343 2939 3686 3834
Size: 867 440 232 804 596 747 148
Fragments arranged by size:
867 804 747 596 440 232 148
EarI CTCTTCn'nnn_
Cuts at: 0 611 2069 2216 3834
Size: 611 1458 147 1618
Fragments arranged by size:
1618 1458 611 147
EcoICRI GAG'CTC
Cuts at: 0 1251 3297 3834
Size: 1251 2046 537
Fragments arranged by size:
2046 1251 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 368 1201 1344 1436 2138 2533 2792
Size: 119 249 833 143 92 702 395 259
Cuts at: 2792 3040 3166 3340 3429 3439 3834
Size: 248 126 174 89 10 395
Fragments arranged by size:
833 702 395 395 259 249 248 174
143 126 119 92 89 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2515 3834
Size: 2515 1319
EcoO109I rG'GnC_Cy
Cuts at: 0 1635 1996 2667 3745 3834
Size: 1635 361 671 1078 89
Fragments arranged by size:
1635 1078 671 361 89
EcoRI G'AATT_C
Cuts at: 0 275 285 420 715 2026 3715 3834
Size: 275 10 135 295 1311 1689 119
Fragments arranged by size:
1689 1311 295 275 135 119 10
EcoRV GAT'ATC
Cuts at: 0 2804 3834
Size: 2804 1030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1051 1222 3834
Size: 1051 171 2612
Fragments arranged by size:
2612 1051 171
FspI TGC'GCA
Cuts at: 0 3814 3834
Size: 3814 20
HaeII r_GCGC'y
Cuts at: 0 733 2511 3834
Size: 733 1778 1323
Fragments arranged by size:
1778 1323 733
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 1554 1586 1747 1749 1779 1781
Size: 1408 32 114 32 161 2 30 2
Cuts at: 1781 1946 1978 2314 2346 3013 3045 3071 3103
Size: 165 32 336 32 667 32 26 32
Cuts at: 3103 3486 3518 3732 3764 3834
Size: 383 32 214 32 70
Fragments arranged by size:
1408 667 383 336 214 165 161 114
70 32 32 32 32 32 32 32
32 30 26 2 2
HincII GTy'rAC
Cuts at: 0 1745 3642 3834
Size: 1745 1897 192
Fragments arranged by size:
1897 1745 192
HindIII A'AGCT_T
Cuts at: 0 324 3834
Size: 324 3510
MfeI C'AATT_G
Cuts at: 0 911 1775 3834
Size: 911 864 2059
Fragments arranged by size:
2059 911 864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 1614 2084 3834
Size: 1367 247 470 1750
Fragments arranged by size:
1750 1367 470 247
MscI TGG'CCA
Cuts at: 0 869 1309 1541 2345 3834
Size: 869 440 232 804 1489
Fragments arranged by size:
1489 869 804 440 232
MslI CAynn'nnrTG
Cuts at: 0 2176 2428 2731 3739 3834
Size: 2176 252 303 1008 95
Fragments arranged by size:
2176 1008 303 252 95
MspA1I CmG'CkG
Cuts at: 0 589 1343 1642 2419 2428 3834
Size: 589 754 299 777 9 1406
Fragments arranged by size:
1406 777 754 589 299 9
NcoI C'CATG_G
Cuts at: 0 860 1556 3236 3505 3834
Size: 860 696 1680 269 329
Fragments arranged by size:
1680 860 696 329 269
NheI G'CTAG_C
Cuts at: 0 3363 3834
Size: 3363 471
NspI r_CATG'y
Cuts at: 0 3129 3834
Size: 3129 705
PflMI CCAn_nnn'nTGG
Cuts at: 0 1481 1596 3834
Size: 1481 115 2238
Fragments arranged by size:
2238 1481 115
PfoI T'CCnGG_A
Cuts at: 0 2602 3834
Size: 2602 1232
PmlI CAC'GTG
Cuts at: 0 469 3834
Size: 469 3365
PpuMI rG'GwC_Cy
Cuts at: 0 1635 2667 3834
Size: 1635 1032 1167
Fragments arranged by size:
1635 1167 1032
PshAI GACnn'nnGTC
Cuts at: 0 2419 3834
Size: 2419 1415
PstI C_TGCA'G
Cuts at: 0 1332 3834
Size: 1332 2502
PvuII CAG'CTG
Cuts at: 0 589 1642 2419 2428 3834
Size: 589 1053 777 9 1406
Fragments arranged by size:
1406 1053 777 589 9
SacI G_AGCT'C
Cuts at: 0 1253 3299 3834
Size: 1253 2046 535
Fragments arranged by size:
2046 1253 535
SapI GCTCTTCn'nnn_
Cuts at: 0 2216 3834
Size: 2216 1618
ScaI AGT'ACT
Cuts at: 0 3371 3442 3834
Size: 3371 71 392
Fragments arranged by size:
3371 392 71
SfcI C'TryA_G
Cuts at: 0 1328 3834
Size: 1328 2506
SmlI C'TyrA_G
Cuts at: 0 554 1168 1207 3834
Size: 554 614 39 2627
Fragments arranged by size:
2627 614 554 39
SpeI A'CTAG_T
Cuts at: 0 1750 3834
Size: 1750 2084
SspI AAT'ATT
Cuts at: 0 1848 3463 3834
Size: 1848 1615 371
Fragments arranged by size:
1848 1615 371
StuI AGG'CCT
Cuts at: 0 1659 3834
Size: 1659 2175
StyI C'CwwG_G
Cuts at: 0 860 1279 1304 1556 1939 2138 3236 3338
Size: 860 419 25 252 383 199 1098 102
Cuts at: 3338 3505 3795 3834
Size: 167 290 39
Fragments arranged by size:
1098 860 419 383 290 252 199 167
102 39 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1569 2295 3834
Size: 1569 726 1539
Fragments arranged by size:
1569 1539 726
TatI w'GTAC_w
Cuts at: 0 492 1988 3278 3369 3440 3834
Size: 492 1496 1290 91 71 394
Fragments arranged by size:
1496 1290 492 394 91 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 3834
Size: 28 32 3774
Fragments arranged by size:
3774 32 28
Tth111I GACn'n_nGTC
Cuts at: 0 1761 3834
Size: 1761 2073
XbaI T'CTAG_A
Cuts at: 0 2332 3834
Size: 2332 1502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 208 3453 3570 3834
Size: 208 3245 117 264
Fragments arranged by size:
3245 264 208 117
XmnI GAAnn'nnTTC
Cuts at: 0 686 3834
Size: 686 3148
ZraI GAC'GTC
Cuts at: 0 3029 3834
Size: 3029 805
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AloI AlwNI
ApaLI ApoI AvaI AvrII BanI BanII BbsI BbvCI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NheI NspI PflMI PfoI PmlI PpuMI
PshAI PstI PvuII SacI SapI ScaI SfcI SmlI
SpeI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaI
AscI AseI AsiSI BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BglII BsiEI BsiWI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I CspCI DraI EagI EciI
FseI FspAI HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI XhoI XmaI