################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 1150 GAATTC [GA]^AATT[CT] Alw44I 346 GTGCAC G^TGCAC AlwNI 998 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 346 GTGCAC G^TGCAC ApoI 1150 GAATTC [GA]^AATT[CT] AviII 470 TGCGCA TGC^GCA BamHI 59 GGATCC G^GATCC BbsI 28 GTCTTC GAAGAC(2/6) BglI 1006 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 333 CTCCAG CTGGAG(16/14) BpuAI 28 GTCTTC GAAGAC(2/6) BsaI 892 GAGACC GGTCTC(1/5) BsgI 328 CTGCAC GTGCAG(16/14) BsiEI 35 CGGCCG CG[GA][CT]^CG BsmBI 463 GAGACG CGTCTC(1/5) Bsp1407I 701 TGTACA T^GTACA BsrBI 22 GAGCGG CCGCTC(-3/-3) BsrDI 159 CATTGC GCAATG(2/0) BsrGI 701 TGTACA T^GTACA BstBI 169 TTCGAA TT^CGAA EagI 35 CGGCCG C^GGCCG EarI 210 GAAGAG CTCTTC(1/4) EclXI 35 CGGCCG C^GGCCG Eco52I 35 CGGCCG C^GGCCG EcoNI 237 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1150 GAATTC G^AATTC FspI 470 TGCGCA TGC^GCA Ksp632I 210 GAAGAG CTCTTC(1/4) KspI 496 CCGCGG CCGC^GG NaeI 1006 GCCGGC GCC^GGC NgoMIV 1006 GCCGGC G^CCGGC NlaIII 678 CATG CATG^ NspV 169 TTCGAA TT^CGAA PpuMI 216 GGGACCC [GA]G^G[AT]CC[CT] PstI 11 CTGCAG CTGCA^G SacII 496 CCGCGG CCGC^GG SexAI 83 ACCTGGT A^CC[AT]GGT SfcI 11 CTGCAG C^T[GA][CT]AG SfuI 169 TTCGAA TT^CGAA SgrAI 1038 CGCCGGTG C[GA]^CCGG[CT]G SmaI 762 CCCGGG CCC^GGG SmlI 355 CTCAAG C^T[CT][GA]AG SspBI 701 TGTACA T^GTACA StuI 752 AGGCCT AGG^CCT TfiI 40 GAATC G^A[AT]TC Tsp509I 1151 AATT ^AATT XmaCI 762 CCCGGG C^CCGGG XmaI 762 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AocI 988 CCTGAGG CC^T[ACGT]AGG 1162 CCTGAGG AspHI 346 GTGCAC G[AT]GC[AT]^C 934 GTGCTC AvaII 217 GGACC G^G[AT]CC 411 GGACC BbvCI 755 CCTCAGC CCTCAGC(-5/-2) 748 GCTGAGG BsiHKAI 346 GTGCAC G[AT]GC[AT]^C 934 GTGCTC BsmAI 463 GAGAC GTCTC(1/5) 892 GAGAC BsrI 1070 ACTGG ACTGG(1/-1) 308 CCAGT BstYI 59 GGATCC [GA]^GATC[CT] 668 AGATCC Bsu36I 988 CCTGAGG CC^T[ACGT]AGG 1162 CCTGAGG DsaI 496 CCGCGG C^C[GA][CT]GG 1022 CCACGG EaeI 35 CGGCCG [CT]^GGCC[GA] 782 TGGCCG Eco81I 988 CCTGAGG CC^T[ACGT]AGG 1162 CCTGAGG MboII 210 GAAGA GAAGA(8/7) 29 TCTTC MflI 59 GGATCC [GA]^GATC[CT] 668 AGATCC PleI 148 GACTC GAGTC(4/5) 958 GAGTC PvuII 915 CAGCTG CAG^CTG 998 CAGCTG XhoII 59 GGATCC [GA]^GATC[CT] 668 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfoI TaqI TseI Tsp45I TspRI TthHB8I