(Circular) (Six-base) MAPSORT of: pFN21AE3544.seq Check: 4819 from: 1 to: 5941 pFN21AE3544 5941 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2558 4004 5388 5656 1632 Size: 635 291 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 635 291 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 3008 3684 5256 3008 Size: 676 1572 3693 Fragments arranged by size: 3693 1572 676 ApaI G_GGCC'C Cuts at: 2211 2260 2774 2211 Size: 49 514 5378 Fragments arranged by size: 5378 514 49 ApaLI G'TGCA_C Cuts at: 2351 3998 5154 2351 Size: 1647 1156 3138 Fragments arranged by size: 3138 1647 1156 ApoI r'AATT_y Cuts at: 1493 3155 3250 3476 3628 5737 1493 Size: 1662 95 226 152 2109 1697 Fragments arranged by size: 2109 1697 1662 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2767 2894 2960 3062 3083 3266 Size: 73 192 632 127 66 102 21 183 Cuts at: 3266 1870 Size: 4545 Fragments arranged by size: 4545 632 192 183 127 102 73 66 21 BamHI G'GATC_C Cuts at: 2064 3271 3638 2064 Size: 1207 367 4367 Fragments arranged by size: 4367 1207 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2570 2693 2986 3019 3047 Size: 325 217 627 783 123 293 33 28 Cuts at: 3047 3078 3203 3262 4717 618 Size: 31 125 59 1455 1842 Fragments arranged by size: 1842 1455 783 627 325 293 217 125 123 59 33 31 28 BanII G_rGCy'C Cuts at: 1391 2136 2211 2260 2511 2774 2909 3042 3063 Size: 745 75 49 251 263 135 133 21 Cuts at: 3063 3260 1391 Size: 197 4072 Fragments arranged by size: 4072 745 263 251 197 135 133 75 49 21 BbeI G_GCGC'C Cuts at: 2574 2697 3082 3207 2574 Size: 123 385 125 5308 Fragments arranged by size: 5308 385 125 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2026 928 Size: 971 127 4843 Fragments arranged by size: 4843 971 127 BbvCI CC'TCA_GC Cuts at: 2754 2761 2754 Size: 7 5934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3017 4552 136 Size: 107 122 71 631 605 1345 1535 1525 Fragments arranged by size: 1535 1525 1345 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2211 2260 2355 2774 2989 4002 5158 1790 Size: 421 49 95 419 215 1013 1156 2573 Fragments arranged by size: 2573 1156 1013 421 419 215 95 49 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2323 4601 1239 Size: 1084 2278 2579 Fragments arranged by size: 2579 2278 1084 Bpu10I CC'TnA_GC Cuts at: 2754 2761 3036 2754 Size: 7 275 5659 Fragments arranged by size: 5659 275 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 4066 4931 5229 2345 Size: 1721 865 298 3057 Fragments arranged by size: 3057 1721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2891 4604 882 Size: 617 1392 1713 2219 Fragments arranged by size: 2219 1713 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2571 2694 2811 3079 Size: 53 83 186 936 1038 123 117 268 Cuts at: 3079 3204 3748 4130 4765 275 Size: 125 544 382 635 1451 Fragments arranged by size: 1451 1038 936 635 544 382 268 186 125 123 117 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5716 Fragments arranged by size: 5716 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2311 2818 2879 3043 1186 Size: 967 158 507 61 164 4084 Fragments arranged by size: 4084 967 507 164 158 61 BseYI C'CCAG_C Cuts at: 2918 3068 3191 3209 5144 5640 2918 Size: 150 123 18 1935 496 3219 Fragments arranged by size: 3219 1935 496 150 123 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 1163 Size: 573 582 4786 Fragments arranged by size: 4786 582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 3391 4152 5180 1540 Size: 275 186 42 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2355 2943 4002 4087 5158 2355 Size: 588 1059 85 1071 3138 Fragments arranged by size: 3138 1071 1059 588 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2462 2462 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2211 2260 2355 2511 2774 2909 Size: 399 346 75 49 95 156 263 135 Cuts at: 2909 2943 2989 3042 3063 3260 4002 4087 5158 Size: 34 46 53 21 197 742 85 1071 Cuts at: 5158 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 346 263 197 156 135 95 85 75 53 49 46 34 21 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2029 3828 1394 Size: 429 206 1799 3507 Fragments arranged by size: 3507 1799 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 2163 4436 4610 65 Size: 1183 915 2273 174 1396 Fragments arranged by size: 2273 1396 1183 915 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2355 3011 3044 4585 4772 1700 Size: 110 545 656 33 1541 187 2869 Fragments arranged by size: 2869 1541 656 545 187 110 33 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3331 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 2175 4745 2175 Size: 2570 3371 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2064 2673 3271 3638 4023 4040 5492 5578 Size: 92 609 598 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 609 598 385 367 346 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2994 3168 2994 Size: 174 5767 BtgI C'CryG_G Cuts at: 513 1065 2004 2501 3027 513 Size: 552 939 497 526 3427 Fragments arranged by size: 3427 939 552 526 497 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2025 3457 4251 4271 2025 Size: 1432 794 20 3695 Fragments arranged by size: 3695 1432 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2787 3388 Size: 54 1425 50 275 18 210 747 601 Cuts at: 3388 4277 5906 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 747 601 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 3388 1537 Size: 275 228 1348 4090 Fragments arranged by size: 4090 1348 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 3871 1353 Size: 857 1661 3423 Fragments arranged by size: 3423 1661 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2077 2363 2666 2706 2902 4528 Size: 1064 138 488 286 303 40 196 1626 Cuts at: 4528 4902 5048 387 Size: 374 146 1280 Fragments arranged by size: 1626 1280 1064 488 374 303 286 196 146 138 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2323 2558 4004 4601 5388 5656 Size: 393 635 56 235 1446 597 787 268 Cuts at: 5656 1239 Size: 1524 Fragments arranged by size: 1524 1446 787 635 597 393 268 235 56 EcoNI CCTnn'n_nnAGG Cuts at: 2246 2246 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2222 2269 2771 2222 Size: 47 502 5392 Fragments arranged by size: 5392 502 47 EcoRI G'AATT_C Cuts at: 3155 3250 3628 3155 Size: 95 378 5468 Fragments arranged by size: 5468 378 95 FspI TGC'GCA Cuts at: 2477 4447 2477 Size: 1970 3971 FspAI rTGC'GCAy Cuts at: 2477 2477 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2313 2559 2574 2594 2697 3082 3207 5088 Size: 635 246 15 20 103 385 125 1881 Cuts at: 5088 1678 Size: 2531 Fragments arranged by size: 2531 1881 635 385 246 125 103 20 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373 Size: 32 135 32 3010 32 42 32 2626 Fragments arranged by size: 3010 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2570 2693 3078 3203 2570 Size: 123 385 125 5308 Fragments arranged by size: 5308 385 125 123 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3899 4258 4417 518 Size: 648 2733 359 159 2042 Fragments arranged by size: 2733 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2379 2503 2922 3005 3209 3357 4034 5182 Size: 773 124 419 83 204 148 677 1148 Cuts at: 5182 5427 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 773 677 419 245 204 148 124 83 NaeI GCC'GGC Cuts at: 3013 3013 Size: 5941 NarI GG'CG_CC Cuts at: 2571 2694 3079 3204 2571 Size: 123 385 125 5308 Fragments arranged by size: 5308 385 125 123 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 3011 3011 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 2774 1348 Size: 1426 4515 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PfoI T'CCnGG_A Cuts at: 2148 2148 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 2222 Size: 5941 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2207 2256 2770 2207 Size: 49 514 5378 Fragments arranged by size: 5378 514 49 PstI C_TGCA'G Cuts at: 830 2020 3293 830 Size: 1190 1273 3478 Fragments arranged by size: 3478 1273 1190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2922 3005 2922 Size: 83 5858 SacII CC_GC'GG Cuts at: 2504 2504 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2222 2222 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SexAI A'CCwGG_T Cuts at: 2088 2088 Size: 5941 SfcI C'TryA_G Cuts at: 826 1045 2016 3289 4424 5105 5296 826 Size: 219 971 1273 1135 681 191 1471 Fragments arranged by size: 1471 1273 1135 971 681 219 191 SfoI GGC'GCC Cuts at: 2572 2695 3080 3205 2572 Size: 123 385 125 5308 Fragments arranged by size: 5308 385 125 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 3044 1700 Size: 1344 4597 SmaI CCC'GGG Cuts at: 1872 2769 3268 1872 Size: 897 499 4545 Fragments arranged by size: 4545 897 499 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 4045 4946 5208 820 Size: 197 926 417 1685 901 262 1553 Fragments arranged by size: 1685 1553 926 901 417 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2759 1517 Size: 1242 4699 StyI C'CwwG_G Cuts at: 513 1065 2004 3380 513 Size: 552 939 1376 3074 Fragments arranged by size: 3074 1376 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2706 4187 Size: 274 80 33 51 1443 141 588 1481 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1481 1443 588 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 5901 5933 2375 Size: 32 3494 32 2383 Fragments arranged by size: 3494 2383 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2767 3266 1870 Size: 897 499 4545 Fragments arranged by size: 4545 897 499 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI XcmI