(Linear) (Six-base) MAPSORT of: KIEE3544.seq Check: 2748 from: 1 to: 1236 KIEE3544.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 553 1236 Size: 262 291 683 Fragments arranged by size: 683 291 262 AlwNI CAG_nnn'CTG Cuts at: 0 1003 1236 Size: 1003 233 ApaI G_GGCC'C Cuts at: 0 206 255 769 1236 Size: 206 49 514 467 Fragments arranged by size: 514 467 206 49 ApaLI G'TGCA_C Cuts at: 0 346 1236 Size: 346 890 ApoI r'AATT_y Cuts at: 0 1150 1236 Size: 1150 86 AvaI C'yCGr_G Cuts at: 0 130 762 889 955 1057 1078 1236 Size: 130 632 127 66 102 21 158 Fragments arranged by size: 632 158 130 127 102 66 21 BamHI G'GATC_C Cuts at: 0 59 1236 Size: 59 1177 BanI G'GyrC_C Cuts at: 0 565 688 981 1014 1042 1073 1198 1236 Size: 565 123 293 33 28 31 125 38 Fragments arranged by size: 565 293 125 123 38 33 31 28 BanII G_rGCy'C Cuts at: 0 131 206 255 506 769 904 1037 1058 Size: 131 75 49 251 263 135 133 21 Cuts at: 1058 1236 Size: 178 Fragments arranged by size: 263 251 178 135 133 131 75 49 21 BbeI G_GCGC'C Cuts at: 0 569 692 1077 1202 1236 Size: 569 123 385 125 34 Fragments arranged by size: 569 385 125 123 34 BbsI GAAGACnn'nnnn_ Cuts at: 0 21 1236 Size: 21 1215 BbvCI CC'TCA_GC Cuts at: 0 749 756 1236 Size: 749 7 480 Fragments arranged by size: 749 480 7 BglI GCCn_nnn'nGGC Cuts at: 0 1012 1236 Size: 1012 224 Bme1580I G_kGCm'C Cuts at: 0 206 255 350 769 984 1236 Size: 206 49 95 419 215 252 Fragments arranged by size: 419 252 215 206 95 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 1236 Size: 318 918 Bpu10I CC'TnA_GC Cuts at: 0 749 756 1031 1236 Size: 749 7 275 205 Fragments arranged by size: 749 275 205 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1236 Size: 340 896 BsaI GGTCTCn'nnnn_ Cuts at: 0 886 1236 Size: 886 350 BsaHI Gr'CG_yC Cuts at: 0 566 689 806 1074 1199 1236 Size: 566 123 117 268 125 37 Fragments arranged by size: 566 268 125 123 117 37 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 148 306 813 874 1038 1236 Size: 148 158 507 61 164 198 Fragments arranged by size: 507 198 164 158 148 61 BseYI C'CCAG_C Cuts at: 0 913 1063 1186 1204 1236 Size: 913 150 123 18 32 Fragments arranged by size: 913 150 123 32 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 1236 Size: 313 923 BsiEI CG_ry'CG Cuts at: 0 38 1236 Size: 38 1198 BsiHKAI G_wGCw'C Cuts at: 0 350 938 1236 Size: 350 588 298 Fragments arranged by size: 588 350 298 BsmBI CGTCTCn'nnnn_ Cuts at: 0 457 1236 Size: 457 779 Bsp1286I G_dGCh'C Cuts at: 0 131 206 255 350 506 769 904 938 Size: 131 75 49 95 156 263 135 34 Cuts at: 938 984 1037 1058 1236 Size: 46 53 21 178 Fragments arranged by size: 263 178 156 135 131 95 75 53 49 46 34 21 BsrBI CCG'CTC Cuts at: 0 24 1236 Size: 24 1212 BsrDI GCAATG_nn' Cuts at: 0 158 1236 Size: 158 1078 BsrFI r'CCGG_y Cuts at: 0 350 1006 1039 1236 Size: 350 656 33 197 Fragments arranged by size: 656 350 197 33 BsrGI T'GTAC_A Cuts at: 0 701 1236 Size: 701 535 BstBI TT'CG_AA Cuts at: 0 170 1236 Size: 170 1066 BstYI r'GATC_y Cuts at: 0 59 668 1236 Size: 59 609 568 Fragments arranged by size: 609 568 59 Bsu36I CC'TnA_GG Cuts at: 0 989 1163 1236 Size: 989 174 73 Fragments arranged by size: 989 174 73 BtgI C'CryG_G Cuts at: 0 496 1022 1236 Size: 496 526 214 Fragments arranged by size: 526 496 214 BtsI GCAGTG_nn' Cuts at: 0 20 1236 Size: 20 1216 EaeI y'GGCC_r Cuts at: 0 35 782 1236 Size: 35 747 454 Fragments arranged by size: 747 454 35 EagI C'GGCC_G Cuts at: 0 35 1236 Size: 35 1201 EarI CTCTTCn'nnn_ Cuts at: 0 205 1236 Size: 205 1031 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 72 358 661 701 897 1236 Size: 72 286 303 40 196 339 Fragments arranged by size: 339 303 286 196 72 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 318 553 1236 Size: 262 56 235 683 Fragments arranged by size: 683 262 235 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 241 1236 Size: 241 995 EcoO109I rG'GnC_Cy Cuts at: 0 217 264 766 1236 Size: 217 47 502 470 Fragments arranged by size: 502 470 217 47 EcoRI G'AATT_C Cuts at: 0 1150 1236 Size: 1150 86 FspI TGC'GCA Cuts at: 0 472 1236 Size: 472 764 FspAI rTGC'GCAy Cuts at: 0 472 1236 Size: 472 764 HaeII r_GCGC'y Cuts at: 0 308 554 569 589 692 1077 1202 1236 Size: 308 246 15 20 103 385 125 34 Fragments arranged by size: 385 308 246 125 103 34 20 15 KasI G'GCGC_C Cuts at: 0 565 688 1073 1198 1236 Size: 565 123 385 125 38 Fragments arranged by size: 565 385 125 123 38 MspA1I CmG'CkG Cuts at: 0 374 498 917 1000 1204 1236 Size: 374 124 419 83 204 32 Fragments arranged by size: 419 374 204 124 83 32 NaeI GCC'GGC Cuts at: 0 1008 1236 Size: 1008 228 NarI GG'CG_CC Cuts at: 0 566 689 1074 1199 1236 Size: 566 123 385 125 37 Fragments arranged by size: 566 385 125 123 37 NgoMIV G'CCGG_C Cuts at: 0 1006 1236 Size: 1006 230 PasI CC'CwG_GG Cuts at: 0 769 1236 Size: 769 467 PfoI T'CCnGG_A Cuts at: 0 143 1236 Size: 143 1093 PpuMI rG'GwC_Cy Cuts at: 0 217 1236 Size: 217 1019 PspOMI G'GGCC_C Cuts at: 0 202 251 765 1236 Size: 202 49 514 471 Fragments arranged by size: 514 471 202 49 PstI C_TGCA'G Cuts at: 0 15 1236 Size: 15 1221 PvuII CAG'CTG Cuts at: 0 917 1000 1236 Size: 917 83 236 Fragments arranged by size: 917 236 83 SacII CC_GC'GG Cuts at: 0 499 1236 Size: 499 737 SanDI GG'GwC_CC Cuts at: 0 217 1236 Size: 217 1019 SexAI A'CCwGG_T Cuts at: 0 83 1236 Size: 83 1153 SfcI C'TryA_G Cuts at: 0 11 1236 Size: 11 1225 SfoI GGC'GCC Cuts at: 0 567 690 1075 1200 1236 Size: 567 123 385 125 36 Fragments arranged by size: 567 385 125 123 36 SgrAI Cr'CCGG_yG Cuts at: 0 1039 1236 Size: 1039 197 SmaI CCC'GGG Cuts at: 0 764 1236 Size: 764 472 SmlI C'TyrA_G Cuts at: 0 355 1236 Size: 355 881 StuI AGG'CCT Cuts at: 0 754 1236 Size: 754 482 TatI w'GTAC_w Cuts at: 0 113 701 1236 Size: 113 588 535 Fragments arranged by size: 588 535 113 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 370 402 1236 Size: 370 32 834 Fragments arranged by size: 834 370 32 XmaI C'CCGG_G Cuts at: 0 762 1236 Size: 762 474 Enzymes that do cut: AcuI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstBI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII KasI MspA1I NaeI NarI NgoMIV PasI PfoI PpuMI PspOMI PstI PvuII SacII SanDI SexAI SfcI SfoI SgrAI SmaI SmlI StuI TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BspMI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FalI FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SfiI SgfI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI