Restriction map for
pFN21AE6196
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# NO CUT ENZYMES ( 199 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI Sau96I
SbfI ScaI ScrFI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 249 GTCGAC GT^[AC][GT]AC
AciI 83 CCGC CCGC(-3/-1)
AfaI 93 GTAC GT^AC
AlwI 135 GATCC GGATC(4/5)
AlwNI 151 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 176 GTGCTC G[AT]GC[AT]^C
BalI 236 TGGCCA TGG^CCA
BbsI 30 GAAGAC GAAGAC(2/6)
BbvI 52 GCTGC GCAGC(8/12)
BfaI 140 CTAG C^TAG
BmyI 176 GTGCTC G[AGT]GC[ACT]^C
BpuAI 30 GAAGAC GAAGAC(2/6)
BsaJI 101 CCACGG C^C[ACGT][ACGT]GG
BsiHKAI 176 GTGCTC G[AT]GC[AT]^C
BsmI 48 GAATGC GAATGC(1/-1)
Bsp1286I 176 GTGCTC G[AGT]GC[ACT]^C
BstYI 134 AGATCC [GA]^GATC[CT]
Cac8I 281 GCAAGC GC[ACGT]^[ACGT]GC
DsaI 101 CCACGG C^C[GA][CT]GG
HincII 249 GTCGAC GT[CT]^[GA]AC
HindII 249 GTCGAC GT[CT]^[GA]AC
HindIII 215 AAGCTT A^AGCTT
HphI 45 GGTGA GGTGA(8/7)
MaeI 140 CTAG C^TAG
MaeII 253 ACGT A^CGT
MboII 30 GAAGA GAAGA(8/7)
MflI 134 AGATCC [GA]^GATC[CT]
MluNI 236 TGGCCA TGG^CCA
MscI 236 TGGCCA TGG^CCA
MseI 209 TTAA T^TAA
MslI 240 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 281 GCAAGCGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RsaI 93 GTAC GT^AC
SalI 249 GTCGAC G^TCGAC
SfaNI 290 GCATC GCATC(5/9)
Tru9I 209 TTAA T^TAA
TseI 52 GCTGC G^C[AT]GC
Tsp45I 58 GTGAC ^GT[GC]AC
XcmI 101 CCACGGTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 134 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BsiYI 36 CCCAGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
83 CCGCATTTTGG
BslI 36 CCCAGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
83 CCGCATTTTGG
BsrI 42 ACTGG ACTGG(1/-1)
184 ACTGG
DpnII 135 GATC ^GATC
273 GATC
EaeI 80 TGGCCG [CT]^GGCC[GA]
236 TGGCCA
Fnu4HI 52 GCTGC GC^[ACGT]GC
82 GCCGC
HaeIII 81 GGCC GG^CC
237 GGCC
ItaI 52 GCTGC GC^[ACGT]GC
82 GCCGC
MaeIII 17 GTAAC ^GT[ACGT]AC
58 GTGAC
MboI 135 GATC ^GATC
273 GATC
NdeII 135 GATC ^GATC
273 GATC
NlaIV 3 GGCTCC GG[ACGT]^[ACGT]CC
114 GGTTCC
Sau3AI 135 GATC ^GATC
273 GATC
TaqI 75 TCGA T^CGA
250 TCGA
TspRI 41 CACTG CA[GC]TG[ACGT][ACGT]^
240 CACTG
TthHB8I 75 TCGA T^CGA
250 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
AluI DdeI MnlI