################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 272 GGTACC G^GTACC AccII 14 CGCG CG^CG AocI 381 CCTCAGG CC^T[ACGT]AGG Asp718I 272 GGTACC G^GTACC BanI 272 GGTACC G^G[CT][GA]CC BcgI 294 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsgI 132 CTGCAC GTGCAG(16/14) BsrBI 268 GAGCGG CCGCTC(-3/-3) BstF5I 93 GGATG GGATG(2/0) BstUI 14 CGCG CG^CG Bsu36I 381 CCTCAGG CC^T[ACGT]AGG DraII 242 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 182 CCGTGG C^C[GA][CT]GG EaeI 290 TGGCCG [CT]^GGCC[GA] Eco81I 381 CCTCAGG CC^T[ACGT]AGG EcoO109I 242 GGGTCCT [GA]G^G[ACGT]CC[CT] FauI 143 CCCGC CCCGC(4/6) Fnu4HI 375 GCGGC GC^[ACGT]GC FokI 93 GGATG GGATG(9/13) HapII 357 CCGG C^CGG HinfI 121 GAGTC G^A[ACGT]TC HpaII 357 CCGG C^CGG HphI 284 TCACC GGTGA(8/7) ItaI 375 GCGGC GC^[ACGT]GC KpnI 272 GGTACC GGTAC^C MspI 357 CCGG C^CGG MvnI 14 CGCG CG^CG MwoI 339 GCTCGCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 312 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 121 GAGTC GAGTC(4/5) PpuMI 242 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 237 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Van91I 312 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 278 CCATGTTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BbvCI 246 CCTCAGC CCTCAGC(-5/-2) 257 CCTCAGC BpmI 118 CTGGAG CTGGAG(16/14) 197 CTGGAG BsmAI 200 GAGAC GTCTC(1/5) 217 GAGAC Cac8I 80 GCCAGC GC[ACGT]^[ACGT]GC 339 GCTCGC EcoNI 288 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 364 CCTCGCACAGG NlaIII 279 CATG CATG^ 301 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AfaI AluI AvaII BsaJI BseRI BsiYI BslI BssKI BstNI Cfr13I DdeI EcoRII HaeIII MnlI MvaI NlaIV RsaI Sau96I ScrFI TspRI