################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 912 GCGG CCGC(-3/-1) AcsI 94 GAATTT [GA]^AATT[CT] AflIII 754 ACATGT A^C[GA][CT]GT ApoI 94 GAATTT [GA]^AATT[CT] AseI 75 ATTAAT AT^TAAT AsnI 75 ATTAAT AT^TAAT Asp700I 367 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 761 GAGCTC G[AT]GC[AT]^C BalI 704 TGGCCA TGG^CCA BamHI 544 GGATCC G^GATCC BbvI 382 GCTGC GCAGC(8/12) BpmI 773 CTGGAG CTGGAG(16/14) BsaAI 613 CACGTA [CT]AC^GT[GA] BsaBI 19 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 816 GAGACC GGTCTC(1/5) BsiHKAI 761 GAGCTC G[AT]GC[AT]^C BsiYI 178 CCGATCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 178 CCGATCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 816 GAGAC GTCTC(1/5) BsmFI 793 GGGAC GGGAC(10/14) BsmI 367 GAATGC GAATGC(1/-1) BspLU11I 754 ACATGT A^CATGT BsrDI 385 GCAATG GCAATG(2/0) BsrI 772 ACTGG ACTGG(1/-1) BssKI 291 CCTGG ^CC[ACGT]GG BstNI 291 CCTGG CC^[AT]GG Cac8I 487 GCTGGC GC[ACGT]^[ACGT]GC EaeI 704 TGGCCA [CT]^GGCC[GA] EarI 802 CTCTTC CTCTTC(1/4) EcoRII 291 CCTGG ^CC[AT]GG Fnu4HI 382 GCTGC GC^[ACGT]GC HaeIII 705 GGCC GG^CC ItaI 382 GCTGC GC^[ACGT]GC Ksp632I 802 CTCTTC CTCTTC(1/4) MaeII 614 ACGT A^CGT MamI 19 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 704 TGGCCA TGG^CCA MscI 704 TGGCCA TGG^CCA MvaI 291 CCTGG CC^[AT]GG MwoI 382 GCTGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PleI 452 GAGTC GAGTC(4/5) PshBI 75 ATTAAT AT^TAAT SacI 761 GAGCTC GAGCT^C ScrFI 291 CCTGG CC^[ACGT]GG SfaNI 717 GATGC GCATC(5/9) SspI 861 AATATT AAT^ATT TseI 382 GCTGC G^C[AT]GC XmnI 367 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanII 645 GGGCTC G[GA]GC[CT]^C 761 GAGCTC BbsI 109 GTCTTC GAAGAC(2/6) 745 GTCTTC BmyI 645 GGGCTC G[AGT]GC[ACT]^C 761 GAGCTC BpuAI 109 GTCTTC GAAGAC(2/6) 745 GTCTTC BseRI 581 CTCCTC GAGGAG(10/8) 735 GAGGAG Bsp1286I 645 GGGCTC G[AGT]GC[ACT]^C 761 GAGCTC BstF5I 243 GGATG GGATG(2/0) 322 GGATG BstYI 24 GGATCT [GA]^GATC[CT] 544 GGATCC FokI 243 GGATG GGATG(9/13) 322 GGATG HindIII 34 AAGCTT A^AGCTT 81 AAGCTT MflI 24 GGATCT [GA]^GATC[CT] 544 GGATCC MseI 43 TTAA T^TAA 76 TTAA MslI 807 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 849 CATGATAGTG NlaIV 358 GGAACC GG[ACGT]^[ACGT]CC 544 GGATCC SfcI 257 CTACAG C^T[GA][CT]AG 903 CTACAG TfiI 879 GATTC G^A[AT]TC 891 GAATC Tru9I 43 TTAA T^TAA 76 TTAA XhoII 24 GGATCT [GA]^GATC[CT] 544 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AfaI AluI AlwI DpnII HinfI HphI MaeIII MboI MboII MnlI NdeII NlaIII NspI RsaI Sau3AI Tsp45I Tsp509I TspRI