################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 192 GAATTT [GA]^AATT[CT] AfaI 177 GTAC GT^AC ApaI 680 GGGCCC GGGCC^C ApoI 192 GAATTT [GA]^AATT[CT] Asp700I 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 139 GTGCTC G[AT]GC[AT]^C AvaII 465 GGTCC G^G[AT]CC BpmI 205 CTGGAG CTGGAG(16/14) BsaI 397 GAGACC GGTCTC(1/5) BsiHKAI 139 GTGCTC G[AT]GC[AT]^C BsmFI 569 GGGAC GGGAC(10/14) BsmI 659 GCATTC GAATGC(1/-1) BspMI 578 ACCTGC ACCTGC(4/8) BsrDI 878 GCAATG GCAATG(2/0) BstEII 96 GGTGACC G^GT[ACGT]ACC BstPI 96 GGTGACC G^GT[ACGT]ACC CfoI 722 GCGC GCG^C EcoNI 548 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 96 GGTGACC G^GT[ACGT]ACC EcoT14I 911 CCTTGG C^C[AT][AT]GG HhaI 722 GCGC GCG^C HinP1I 722 GCGC G^CGC HindIII 30 AAGCTT A^AGCTT HphI 96 GGTGA GGTGA(8/7) MaeII 610 ACGT A^CGT MseI 91 TTAA T^TAA MwoI 682 GCCCCTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 447 GCTAGC G^CTAGC PleI 18 GACTC GAGTC(4/5) PpuMI 464 GGGTCCT [GA]G^G[AT]CC[CT] RsaI 177 GTAC GT^AC SfcI 686 CTACAG C^T[GA][CT]AG SpeI 429 ACTAGT A^CTAGT StyI 911 CCTTGG C^C[AT][AT]GG TfiI 455 GATTC G^A[AT]TC Tru9I 91 TTAA T^TAA XcmI 476 CCACTGTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 264 GGATC GGATC(4/5) 360 GGATC AlwNI 70 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 689 CAGCTGCTG BalI 411 TGGCCA TGG^CCA 488 TGGCCA BanI 322 GGCACC G^G[CT][GA]CC 817 GGTGCC BanII 624 GGGCTC G[GA]GC[CT]^C 680 GGGCCC BlpI 141 GCTCAGC GC^T[ACGT]AGC 694 GCTGAGC Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC 694 GCTGAGC BsgI 163 CTGCAC GTGCAG(16/14) 889 GTGCAG BsmAI 397 GAGAC GTCTC(1/5) 354 GTCTC BstF5I 252 CATCC GGATG(2/0) 294 CATCC CelII 141 GCTCAGC GC^T[ACGT]AGC 694 GCTGAGC DpnII 265 GATC ^GATC 361 GATC FokI 252 CATCC GGATG(9/13) 294 CATCC HinfI 18 GACTC G^A[ACGT]TC 455 GATTC MboI 265 GATC ^GATC 361 GATC MluNI 411 TGGCCA TGG^CCA 488 TGGCCA MscI 411 TGGCCA TGG^CCA 488 TGGCCA NdeII 265 GATC ^GATC 361 GATC Sau3AI 265 GATC ^GATC 361 GATC Tsp509I 132 AATT ^AATT 193 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII ItaI MaeI MaeIII MboII MnlI MslI MspA1I MvaI NlaIII NlaIV PvuII Sau96I ScrFI SfaNI TseI Tsp45I TspRI