(Circular) (Six-base) MAPSORT of: pFN21AE6076.seq Check: 587 from: 1 to: 5674 pFN21AE6076 5674 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2592 2592 Size: 5674 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3737 5121 5389 1632 Size: 2105 1384 268 1917 Fragments arranged by size: 2105 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 4573 5550 1203 Size: 3370 977 1327 Fragments arranged by size: 3370 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 2939 1166 Size: 1773 3901 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2080 2699 3417 4989 2080 Size: 619 718 1572 2765 Fragments arranged by size: 2765 1572 718 619 ApaI G_GGCC'C Cuts at: 2689 2689 Size: 5674 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2197 2983 3209 3361 5470 1493 Size: 704 786 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 704 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2999 1870 Size: 73 1056 4545 Fragments arranged by size: 4545 1056 73 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2822 2995 4450 618 Size: 325 217 627 540 495 173 1455 1842 Fragments arranged by size: 1842 1455 627 540 495 325 217 173 BanII G_rGCy'C Cuts at: 1391 2633 2689 2993 1391 Size: 1242 56 304 4072 Fragments arranged by size: 4072 1242 304 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 2147 2700 3091 2147 Size: 553 391 4730 Fragments arranged by size: 4730 553 391 Bme1580I G_kGCm'C Cuts at: 1790 2689 3735 4891 1790 Size: 899 1046 1156 2573 Fragments arranged by size: 2573 1156 1046 899 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 2636 3405 Size: 416 53 83 399 24 635 584 769 Cuts at: 3405 4363 442 Size: 958 1753 Fragments arranged by size: 1753 958 769 635 584 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2456 1056 Size: 1400 4274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 4334 1239 Size: 992 2103 2579 Fragments arranged by size: 2579 2103 992 Bpu10I CC'TnA_GC Cuts at: 2558 2834 2558 Size: 276 5398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3799 4664 4962 3799 Size: 865 298 4511 Fragments arranged by size: 4511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 4337 882 Size: 617 897 1941 2219 Fragments arranged by size: 2219 1941 897 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 3863 4498 275 Size: 53 83 186 936 1948 382 635 1451 Fragments arranged by size: 1948 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5674 BseYI C'CCAG_C Cuts at: 4877 5373 4877 Size: 496 5178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2915 1163 Size: 573 417 762 3922 Fragments arranged by size: 3922 762 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 3735 3820 4891 2148 Size: 1587 85 1071 2931 Fragments arranged by size: 2931 1587 1071 85 BsmI GAATG_Cn' Cuts at: 2664 3185 3278 2664 Size: 521 93 5060 Fragments arranged by size: 5060 521 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2633 2689 2993 3735 3820 4891 Size: 399 358 485 56 304 742 85 1071 Cuts at: 4891 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 485 399 358 304 85 56 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2592 3029 844 Size: 1748 437 3489 Fragments arranged by size: 3489 1748 437 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2890 4169 4343 65 Size: 1183 1642 1279 174 1396 Fragments arranged by size: 1642 1396 1279 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4318 4505 1700 Size: 110 2508 187 2869 Fragments arranged by size: 2869 2508 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 2101 1184 Size: 917 4757 BstYI r'GATC_y Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669 Size: 1032 367 385 17 1452 86 12 346 Cuts at: 5669 1972 Size: 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4935 Fragments arranged by size: 4935 683 56 BtsI GCAGTG_nn' Cuts at: 2934 3190 3984 4004 2934 Size: 256 794 20 4604 Fragments arranged by size: 4604 794 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2416 2493 Size: 54 1425 50 275 18 519 67 77 Cuts at: 2493 3121 4010 5639 8 Size: 628 889 1629 43 Fragments arranged by size: 1629 1425 889 628 519 275 77 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3604 1353 Size: 2251 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 3737 4334 5121 5389 1239 Size: 393 599 1506 597 787 268 1524 Fragments arranged by size: 1524 1506 787 599 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2557 2557 Size: 5674 EcoO109I rG'GnC_Cy Cuts at: 2470 2685 2686 2470 Size: 215 1 5458 Fragments arranged by size: 5458 215 1 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2786 2818 4315 4347 4389 Size: 32 135 32 1214 32 1497 32 42 Cuts at: 4389 4421 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 1214 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 2035 748 Size: 1287 4387 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4788 4972 1305 Size: 3483 184 2007 Fragments arranged by size: 3483 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2418 2495 10 Size: 54 1425 343 586 77 3189 Fragments arranged by size: 3189 1425 586 343 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2786 2939 3632 3991 4150 518 Size: 648 1011 609 153 693 359 159 2042 Fragments arranged by size: 2042 1011 693 648 609 359 159 153 MspA1I CmG'CkG Cuts at: 1606 2151 2292 2696 3090 3767 4915 5160 1606 Size: 545 141 404 394 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 404 394 245 141 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5122 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NheI G'CTAG_C Cuts at: 1052 2452 1052 Size: 1400 4274 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 3032 4577 1132 Size: 97 1803 1545 2229 Fragments arranged by size: 2229 1803 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2470 2470 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PspOMI G'GGCC_C Cuts at: 2685 2685 Size: 5674 PstI C_TGCA'G Cuts at: 830 3026 830 Size: 2196 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2151 2292 2696 2151 Size: 141 404 5129 Fragments arranged by size: 5129 404 141 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2691 3022 4157 4838 5029 826 Size: 219 1646 331 1135 681 191 1471 Fragments arranged by size: 1646 1471 1135 681 331 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3778 4679 4941 820 Size: 197 926 1835 901 262 1553 Fragments arranged by size: 1835 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 2434 152 Size: 2282 3392 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2916 3113 513 Size: 552 1851 197 3074 Fragments arranged by size: 3074 1851 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 3701 3718 3871 4056 4501 325 Size: 1255 1014 1107 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1107 1014 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3920 5632 Size: 274 80 33 51 1443 204 1739 1712 Cuts at: 5632 96 Size: 138 Fragments arranged by size: 1739 1712 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2488 2488 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 2864 3803 2864 Size: 939 4735 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI