################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 568 GTAGAC GT^[AC][GT]AC AciI 859 CCGC CCGC(-3/-1) AflII 873 CTTAAG C^TTAAG AlwNI 238 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 458 TGGCCA TGG^CCA BanII 321 GGGCTC G[GA]GC[CT]^C BfaI 205 CTAG C^TAG BfrI 873 CTTAAG C^TTAAG BglII 207 AGATCT A^GATCT BmyI 321 GGGCTC G[AGT]GC[ACT]^C BpmI 444 CTGGAG CTGGAG(16/14) BsaBI 429 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 807 CCAGGG C^C[ACGT][ACGT]GG BsmAI 851 GTCTC GTCTC(1/5) BsmFI 258 GGGAC GGGAC(10/14) Bsp1286I 321 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 478 TGTACA T^GTACA BspMI 776 ACCTGC ACCTGC(4/8) BsrBI 859 CCGCTC CCGCTC(-3/-3) BsrGI 478 TGTACA T^GTACA DraII 258 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 458 TGGCCA [CT]^GGCC[GA] EcoO109I 258 GGGACCT [GA]G^G[ACGT]CC[CT] FauI 858 CCCGC CCCGC(4/6) HaeIII 459 GGCC GG^CC HincII 247 GTCAAC GT[CT]^[GA]AC HindII 247 GTCAAC GT[CT]^[GA]AC HinfI 154 GATTC G^A[ACGT]TC MaeI 205 CTAG C^TAG MamI 429 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 458 TGGCCA TGG^CCA MscI 458 TGGCCA TGG^CCA MslI 413 CACATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 258 GGGACC GG[ACGT]^[ACGT]CC PpuMI 258 GGGACCT [GA]G^G[AT]CC[CT] SexAI 783 ACCAGGT A^CC[AT]GGT SmlI 873 CTTAAG C^T[CT][GA]AG SspBI 478 TGTACA T^GTACA TfiI 154 GATTC G^A[AT]TC TspRI 834 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 272 CCATCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 192 AAATTT [GA]^AATT[CT] 492 GAATTT ApoI 192 AAATTT [GA]^AATT[CT] 492 GAATTT AvaII 3 GGACC G^G[AT]CC 259 GGACC BsiYI 336 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCCACATGTGG BslI 336 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCCACATGTGG BspHI 404 TCATGA T^CATGA 437 TCATGA BsrDI 660 CATTGC GCAATG(2/0) 709 GCAATG BstYI 207 AGATCT [GA]^GATC[CT] 724 GGATCT Cfr13I 3 GGACC G^G[ACGT]CC 259 GGACC HphI 821 TCACC GGTGA(8/7) 921 TCACC MaeIII 122 GTAAC ^GT[ACGT]AC 788 GTAAC MboII 903 GAAGA GAAGA(8/7) 98 TCTTC MflI 207 AGATCT [GA]^GATC[CT] 724 GGATCT MseI 106 TTAA T^TAA 874 TTAA RcaI 404 TCATGA T^CATGA 437 TCATGA Sau96I 3 GGACC G^G[ACGT]CC 259 GGACC SfcI 467 CTGTAG C^T[GA][CT]AG 737 CTGTAG Tru9I 106 TTAA T^TAA 874 TTAA XhoII 207 AGATCT [GA]^GATC[CT] 724 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AfaI AflIII AluI AlwI BspLU11I BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI MboI MnlI MvaI NdeII NlaIII NspI RsaI Sau3AI ScrFI SfaNI Tsp509I