(Circular) (Six-base) MAPSORT of: pFN21AE6065.seq Check: 1461 from: 1 to: 5665 pFN21AE6065 5665 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 3008 1937 Size: 637 434 4594 Fragments arranged by size: 4594 637 434 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2927 3728 5112 5380 1632 Size: 1295 801 1384 268 1917 Fragments arranged by size: 1917 1384 1295 801 268 AflII C'TTAA_G Cuts at: 820 1017 2878 820 Size: 197 1861 3607 Fragments arranged by size: 3607 1861 197 AflIII A'CryG_T Cuts at: 1203 2316 2507 2775 4564 5541 1203 Size: 1113 191 268 1789 977 1327 Fragments arranged by size: 1789 1327 1113 977 268 191 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 2248 3408 4980 2248 Size: 1160 1572 2933 Fragments arranged by size: 2933 1572 1160 ApaLI G'TGCA_C Cuts at: 3722 4878 3722 Size: 1156 4509 ApoI r'AATT_y Cuts at: 1493 2197 2497 2974 3200 3352 5461 1493 Size: 704 300 477 226 152 2109 1697 Fragments arranged by size: 2109 1697 704 477 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2990 1870 Size: 73 1047 4545 Fragments arranged by size: 4545 1047 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2330 2984 1391 Size: 939 654 4072 Fragments arranged by size: 4072 939 654 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2212 5660 2212 Size: 3448 2217 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 3726 4882 1790 Size: 1936 1156 2573 Fragments arranged by size: 2573 1936 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2470 4325 1239 Size: 1231 1855 2579 Fragments arranged by size: 2579 1855 1231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4655 4953 3790 Size: 865 298 4502 Fragments arranged by size: 4502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 2438 5659 2438 Size: 3221 2444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2443 2473 1178 Size: 30 165 30 1040 30 4370 Fragments arranged by size: 4370 1040 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2163 4868 5364 2163 Size: 2705 496 2464 Fragments arranged by size: 2705 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5092 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3726 3811 4882 3726 Size: 85 1071 4509 Fragments arranged by size: 4509 1071 85 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2984 3726 3811 4882 1391 Size: 399 540 654 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 654 540 399 85 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 2409 2442 3554 5284 2409 Size: 33 1112 1730 2790 Fragments arranged by size: 2790 1730 1112 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2790 3020 844 Size: 1946 230 3489 Fragments arranged by size: 3489 1946 230 BsrBI CCG'CTC Cuts at: 1394 1823 2866 3552 1394 Size: 429 1043 686 3507 Fragments arranged by size: 3507 1043 686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2664 2721 4160 4334 65 Size: 1183 1416 57 1439 174 1396 Fragments arranged by size: 1439 1416 1396 1183 174 57 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 2483 96 Size: 2387 3278 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5665 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2212 2729 2995 3362 3747 3764 5216 5302 Size: 240 517 266 367 385 17 1452 86 Cuts at: 5302 5314 5660 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 517 385 367 346 266 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4174 Fragments arranged by size: 4174 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3975 3995 3181 Size: 794 20 4851 Fragments arranged by size: 4851 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2569 2604 529 Size: 35 2005 35 3590 Fragments arranged by size: 3590 2005 35 35 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 3112 4001 Size: 54 1425 50 275 18 633 649 889 Cuts at: 4001 5630 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 649 633 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3595 1353 Size: 2242 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2470 2927 3728 4325 5112 5380 1239 Size: 393 838 457 801 597 787 268 1524 Fragments arranged by size: 1524 838 801 787 597 457 393 268 EcoO109I rG'GnC_Cy Cuts at: 2264 2264 Size: 5665 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 4812 1678 Size: 3134 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373 Size: 32 135 32 2734 32 42 32 2626 Fragments arranged by size: 2734 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 3009 3255 669 Size: 988 281 316 755 246 3079 Fragments arranged by size: 3079 988 755 316 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2326 4779 4963 1305 Size: 1021 2453 184 2007 Fragments arranged by size: 2453 2007 1021 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 10 Size: 54 1425 343 633 3210 Fragments arranged by size: 3210 1425 633 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2422 3623 3982 4141 518 Size: 648 1256 1201 359 159 2042 Fragments arranged by size: 2042 1256 1201 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3081 3758 4906 5151 1606 Size: 1475 677 1148 245 2120 Fragments arranged by size: 2120 1475 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4174 Fragments arranged by size: 4174 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2320 2511 2779 3023 4568 1132 Size: 97 1091 191 268 244 1545 2229 Fragments arranged by size: 2229 1545 1091 268 244 191 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 2316 2507 2775 4564 2316 Size: 191 268 1789 3417 Fragments arranged by size: 3417 1789 268 191 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2264 2264 Size: 5665 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 2618 3235 2618 Size: 617 5048 PstI C_TGCA'G Cuts at: 830 3017 830 Size: 2187 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SexAI A'CCwGG_T Cuts at: 2788 2788 Size: 5665 SfcI C'TryA_G Cuts at: 826 1045 2472 2742 3013 4148 4829 5020 826 Size: 219 1427 270 271 1135 681 191 1471 Fragments arranged by size: 1471 1427 1135 681 271 270 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2992 1872 Size: 1120 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2878 3769 4670 4932 820 Size: 197 926 935 891 901 262 1553 Fragments arranged by size: 1553 935 926 901 891 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5665 StyI C'CwwG_G Cuts at: 513 1065 2004 3104 513 Size: 552 939 1100 3074 Fragments arranged by size: 3074 1100 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3692 3709 3862 4047 4492 325 Size: 1255 2112 17 153 185 445 1498 Fragments arranged by size: 2112 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2483 3911 Size: 274 80 33 51 1443 204 302 1428 Cuts at: 3911 5623 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1428 302 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 5625 5657 2622 Size: 32 2971 32 2630 Fragments arranged by size: 2971 2630 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2990 1870 Size: 1120 4545 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI