################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI Ksp632I KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 656 TCCGGA T^CCGGA AvaII 793 GGTCC G^G[AT]CC BalI 431 TGGCCA TGG^CCA BbrPI 717 CACGTG CAC^GTG BbsI 826 GTCTTC GAAGAC(2/6) BpuAI 826 GTCTTC GAAGAC(2/6) BsaAI 717 CACGTG [CT]AC^GT[GA] BsaWI 656 TCCGGA [AT]^CCGG[AT] BseAI 656 TCCGGA T^CCGGA BseRI 576 CTCCTC GAGGAG(10/8) BspEI 656 TCCGGA T^CCGGA BspHI 107 TCATGA T^CATGA BstYI 680 AGATCC [GA]^GATC[CT] DraII 895 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 895 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 72 GATATC GAT^ATC EcoT22I 406 ATGCAT ATGCA^T HapII 657 CCGG C^CGG HincII 930 GTTAAC GT[CT]^[GA]AC HindII 930 GTTAAC GT[CT]^[GA]AC HinfI 161 GACTC G^A[ACGT]TC HpaI 930 GTTAAC GTT^AAC HpaII 657 CCGG C^CGG MflI 680 AGATCC [GA]^GATC[CT] MluNI 431 TGGCCA TGG^CCA MroI 656 TCCGGA T^CCGGA MscI 431 TGGCCA TGG^CCA MseI 931 TTAA T^TAA MspI 657 CCGG C^CGG NsiI 406 ATGCAT ATGCA^T PleI 161 GACTC GAGTC(4/5) PmaCI 717 CACGTG CAC^GTG PmlI 717 CACGTG CAC^GTG RcaI 107 TCATGA T^CATGA TaqI 206 TCGA T^CGA Tru9I 931 TTAA T^TAA TthHB8I 206 TCGA T^CGA XcmI 780 CCAGCCCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 680 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 384 CCGC AcsI 31 GAATTT [GA]^AATT[CT] 919 AAATTC AflIII 716 ACACGT A^C[GA][CT]GT 718 ACGTGT Alw44I 63 GTGCAC G^TGCAC 504 GTGCAC AlwI 681 GATCC GGATC(4/5) 659 GGATC AlwNI 54 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 238 CAGCTGCTG ApaLI 63 GTGCAC G^TGCAC 504 GTGCAC ApoI 31 GAATTT [GA]^AATT[CT] 919 AAATTC Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 705 GAAAGCCTTC AspHI 63 GTGCAC G[AT]GC[AT]^C 504 GTGCAC BclI 143 TGATCA T^GATCA 522 TGATCA BmyI 63 GTGCAC G[AGT]GC[ACT]^C 504 GTGCAC BsgI 290 GTGCAG GTGCAG(16/14) 472 GTGCAG BsiHKAI 63 GTGCAC G[AT]GC[AT]^C 504 GTGCAC BsmAI 128 GTCTC GTCTC(1/5) 211 GTCTC BsmFI 232 GTCCC GGGAC(10/14) 794 GTCCC Bsp1286I 63 GTGCAC G[AGT]GC[ACT]^C 504 GTGCAC Cfr13I 793 GGTCC G^G[ACGT]CC 896 GGGCC EaeI 398 CGGCCA [CT]^GGCC[GA] 431 TGGCCA EcoT14I 42 CCTTGG C^C[AT][AT]GG 317 CCTTGG FbaI 143 TGATCA T^GATCA 522 TGATCA MaeII 19 ACGT A^CGT 718 ACGT MaeIII 56 GTGAC ^GT[ACGT]AC 97 GTCAC MspA1I 238 CAGCTG C[AC]G^C[GT]G 493 CAGCTG PflMI 53 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 237 CCAGCTGCTGG PvuII 238 CAGCTG CAG^CTG 493 CAGCTG Sau96I 793 GGTCC G^G[ACGT]CC 896 GGGCC StyI 42 CCTTGG C^C[AT][AT]GG 317 CCTTGG Tsp45I 56 GTGAC ^GT[GC]AC 97 GTCAC Tsp509I 32 AATT ^AATT 920 AATT Van91I 53 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 237 CCAGCTGCTGG XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 705 GAAAGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AluI BbvI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeI MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI TseI TspRI