Restriction map for
pFN21AE6040
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MroI MseI
MslI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmlI SpeI SphI
Sse8387I SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 592 GTAGAC GT^[AC][GT]AC
AciI 725 CCGC CCGC(-3/-1)
AcsI 6 AAATTT [GA]^AATT[CT]
AcyI 698 GACGCC G[GA]^CG[CT]C
ApoI 6 AAATTT [GA]^AATT[CT]
AspHI 826 GTGCTC G[AT]GC[AT]^C
AvaI 785 CCCGGG C^[CT]CG[GA]G
AvrII 934 CCTAGG C^CTAGG
BamHI 680 GGATCC G^GATCC
BlnI 934 CCTAGG C^CTAGG
BmyI 826 GTGCTC G[AGT]GC[ACT]^C
BsaAI 280 TACGTA [CT]AC^GT[GA]
BsaHI 698 GACGCC G[GA]^CG[CT]C
BsiHKAI 826 GTGCTC G[AT]GC[AT]^C
BsmFI 696 GGGAC GGGAC(10/14)
BsmI 123 GAATGC GAATGC(1/-1)
BsoBI 785 CCCGGG C^[CT]CG[GA]G
Bsp1286I 826 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 794 TGTACA T^GTACA
BspMI 562 ACCTGC ACCTGC(4/8)
BsrBI 725 CCGCTC CCGCTC(-3/-3)
BsrDI 663 CATTGC GCAATG(2/0)
BsrGI 794 TGTACA T^GTACA
BsrI 413 CCAGT ACTGG(1/-1)
BstYI 680 GGATCC [GA]^GATC[CT]
EarI 766 CTCTTC CTCTTC(1/4)
EcoNI 255 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 786 CCGG C^CGG
HgaI 698 GACGC GACGC(5/10)
Hin1I 698 GACGCC G[GA]^CG[CT]C
HindIII 475 AAGCTT A^AGCTT
HpaII 786 CCGG C^CGG
Ksp632I 766 CTCTTC CTCTTC(1/4)
MfeI 493 CAATTG C^AATTG
MflI 680 GGATCC [GA]^GATC[CT]
MspA1I 70 CAGCTG C[AC]G^C[GT]G
MspI 786 CCGG C^CGG
MunI 493 CAATTG C^AATTG
PleI 272 GAGTC GAGTC(4/5)
PvuII 70 CAGCTG CAG^CTG
SmaI 785 CCCGGG CCC^GGG
SnaBI 280 TACGTA TAC^GTA
SspBI 794 TGTACA T^GTACA
StuI 896 AGGCCT AGG^CCT
TaqI 26 TCGA T^CGA
TfiI 618 GATTC G^A[AT]TC
TthHB8I 26 TCGA T^CGA
XhoII 680 GGATCC [GA]^GATC[CT]
XmaCI 785 CCCGGG C^CCGGG
XmaI 785 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 681 GATCC GGATC(4/5)
680 GGATC
AocI 675 CCTGAGG CC^T[ACGT]AGG
873 CCTGAGG
AvaII 382 GGACC G^G[AT]CC
508 GGACC
BbsI 906 GAAGAC GAAGAC(2/6)
136 GTCTTC
BcnI 785 CCCGG CC^[GC]GG
786 CCGGG
BpmI 67 CTCCAG CTGGAG(16/14)
205 CTGGAG
BpuAI 906 GAAGAC GAAGAC(2/6)
136 GTCTTC
BsmAI 274 GTCTC GTCTC(1/5)
401 GTCTC
Bsu36I 675 CCTGAGG CC^T[ACGT]AGG
873 CCTGAGG
Cac8I 150 GCTTGC GC[ACGT]^[ACGT]GC
579 GCAAGC
DpnII 361 GATC ^GATC
681 GATC
Eco81I 675 CCTGAGG CC^T[ACGT]AGG
873 CCTGAGG
EcoT14I 186 CCTTGG C^C[AT][AT]GG
934 CCTAGG
HinfI 272 GAGTC G^A[ACGT]TC
618 GATTC
MaeII 281 ACGT A^CGT
688 ACGT
MaeIII 226 GTCAC ^GT[ACGT]AC
926 GTCAC
MboI 361 GATC ^GATC
681 GATC
NciI 785 CCCGG CC^[GC]GG
786 CCGGG
NdeII 361 GATC ^GATC
681 GATC
Sau3AI 361 GATC ^GATC
681 GATC
StyI 186 CCTTGG C^C[AT][AT]GG
934 CCTAGG
Tsp45I 226 GTCAC ^GT[GC]AC
926 GTCAC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BalI BbvI BfaI
BsaJI BseRI BsiYI BslI BssKI
BstF5I BstNI Cfr13I DdeI EaeI
EcoRII Fnu4HI FokI HaeIII HphI
ItaI MaeI MboII MluNI MnlI
MscI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TseI Tsp509I
TspRI