Restriction map for pFN21AE6040
 (Circular) (Six-base) MAPSORT of: pFN21AE6040.seq  Check: 1936  from: 1  to: 5656



pFN21AE6040  5656 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2576    2576 
   Size:       5656
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3466     278 
   Size:         53      83     186     936    1930    2468

  Fragments arranged by size: 

                2468    1930     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2598    2999    1937 
   Size:        661     401    4594

  Fragments arranged by size: 

                4594     661     401
 
Acc65I G'GTAC_C

Cuts at:   2977    2977 
   Size:       5656
 
AclI AA'CG_TT

Cuts at:   1563    3783    4156    1563 
   Size:       2220     373    3063

  Fragments arranged by size: 

                3063    2220     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2449    2890    3719    5103    5371    1632 
   Size:        817     441     829    1384     268    1917

  Fragments arranged by size: 

                1917    1384     829     817     441     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5459
 
AflIII A'CryG_T

Cuts at:   1203    4555    5532    1203 
   Size:       3352     977    1327

  Fragments arranged by size: 

                3352    1327     977
 
AgeI A'CCGG_T

Cuts at:   4487    4487 
   Size:       5656
 
AhdI GACnn_n'nnGTC

Cuts at:   4385    4385 
   Size:       5656
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5656
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5656
 
AlwNI CAG_nnn'CTG

Cuts at:   3399    4971    3399 
   Size:       1572    4084
 
ApaLI G'TGCA_C

Cuts at:   3713    4869    3713 
   Size:       1156    4500
 
ApoI r'AATT_y

Cuts at:   1493    2011    2965    3191    3343    5452    1493 
   Size:        518     954     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     954     518     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4210     160 
   Size:        872    3178    1606

  Fragments arranged by size: 

                3178    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5656
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2790    2981    1870 
   Size:         73     847     191    4545

  Fragments arranged by size: 

                4545     847     191      73
 
AvrII C'CTAG_G

Cuts at:   2939    2939 
   Size:       5656
 
BamHI G'GATC_C

Cuts at:   2685    2986    3353    2685 
   Size:        301     367    4988

  Fragments arranged by size: 

                4988     367     301
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2977    4432     618 
   Size:        325     217     627    1190    1455    1842

  Fragments arranged by size: 

                1842    1455    1190     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2975    1391 
   Size:       1584    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2134    2918     928 
   Size:        971     235     784    3666

  Fragments arranged by size: 

                3666     971     784     235
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3847    3881    1903 
   Size:         34    1910      34    3678

  Fragments arranged by size: 

                3678    1910      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3548    4758    3548 
   Size:       1210    4446
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5656
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4267     136 
   Size:        107     122      71     631     605    2595    1525

  Fragments arranged by size: 

                2595    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5651    5651 
   Size:       5656
 
BlpI GC'TnA_GC

Cuts at:   3073    3073 
   Size:       5656
 
Bme1580I G_kGCm'C

Cuts at:   1790    3717    4873    1790 
   Size:       1927    1156    2573

  Fragments arranged by size: 

                2573    1927    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5656
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3387    4345     442 
   Size:        416      53      83     399      24    1970     958    1753

  Fragments arranged by size: 

                1970    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5656
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2057    2231    4316    1239 
   Size:        818     174    2085    2579

  Fragments arranged by size: 

                2579    2085     818     174
 
Bpu10I CC'TnA_GC

Cuts at:   2323    2323 
   Size:       5656
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3781    4646    4944    3781 
   Size:        865     298    4493

  Fragments arranged by size: 

                4493     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4319     882 
   Size:        617    2820    2219

  Fragments arranged by size: 

                2820    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2287    3447     493 
   Size:        711    1083    1160    2702

  Fragments arranged by size: 

                2702    1160    1083     711
 
BsaBI GATnn'nnATC

Cuts at:   5650    5650 
   Size:       5656
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2704    3463    3845    4480 
   Size:         53      83     186     936    1171     759     382     635

Cuts at:   4480     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1171     936     759     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4089    4487    4761    4908    5319    4089 
   Size:        398     274     147     411    4426

  Fragments arranged by size: 

                4426     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2013    2043    2050    2080    2204 
   Size:         30     165      30     610      30       7      30     124

Cuts at:   2204    2234    2747    2777    1178 
   Size:         30     513      30    4057

  Fragments arranged by size: 

                4057     610     513     165     124      30      30      30
                  30      30      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2048    2072    2752    1186 
   Size:        862      24     680    4090

  Fragments arranged by size: 

                4090     862     680      24
 
BseYI C'CCAG_C

Cuts at:   2863    4859    5355    2863 
   Size:       1996     496    3164

  Fragments arranged by size: 

                3164    1996     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5083
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3106    3867    4895    1540 
   Size:        275     186    1105     761    1028    2301

  Fragments arranged by size: 

                2301    1105    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2835    3717    3802    4873    2835 
   Size:        882      85    1071    3618

  Fragments arranged by size: 

                3618    1071     882      85
 
BsmI GAATG_Cn'

Cuts at:   2134    3167    3260    2134 
   Size:       1033      93    4530

  Fragments arranged by size: 

                4530    1033      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2835    2975    3717    3802    4873    1391 
   Size:        399    1045     140     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071    1045     742     399     140      85
 
BspEI T'CCGG_A

Cuts at:   5319    5319 
   Size:       5656
 
BspHI T'CATG_A

Cuts at:   3545    5275    3545 
   Size:       1730    3926
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2576    3011     844 
   Size:       1732     435    3489

  Fragments arranged by size: 

                3489    1732     435
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2732    3543    1394 
   Size:        429     909     811    3507

  Fragments arranged by size: 

                3507     909     811     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2667    4151    4325      65 
   Size:       1183    1419    1484     174    1396

  Fragments arranged by size: 

                1484    1419    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4300    4487    1700 
   Size:        110    2490     187    2869

  Fragments arranged by size: 

                2869    2490     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2799      96 
   Size:       2703    2953
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5656
 
BssSI C'ACGA_G

Cuts at:   3716    4728    3716 
   Size:       1012    4644
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5656
 
BstBI TT'CG_AA

Cuts at:   4460    4460 
   Size:       5656
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5656
 
BstYI r'GATC_y

Cuts at:   1972    2685    2986    3353    3738    3755    5207    5293    5305 
   Size:        713     301     367     385      17    1452      86      12

Cuts at:   5305    5651    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     713     385     367     346     301      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2681    2879    2681 
   Size:        198    5458
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5104
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2156     487 
   Size:        683      56     930    3987

  Fragments arranged by size: 

                3987     930     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2461    2658    2748    3172    3966    3986    2461 
   Size:        197      90     424     794      20    4131

  Fragments arranged by size: 

                4131     794     424     197      90      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5656
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2416    2451     529 
   Size:         35    1852      35    3734

  Fragments arranged by size: 

                3734    1852      35      35
 
DraI TTT'AAA

Cuts at:   2960    2960 
   Size:       5656
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4663     809 
   Size:        507    3347    1802

  Fragments arranged by size: 

                3347    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2194    2376    2424 
   Size:         54    1425      50     275      18     364     182      48

Cuts at:   2424    3103    3992    5621       8 
   Size:        679     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     679     364     275     182      54
                  50      48      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3103    1537 
   Size:        275    1291    4090

  Fragments arranged by size: 

                4090    1291     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2777    3586    1353 
   Size:       1424     809    3423

  Fragments arranged by size: 

                3423    1424     809
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4243    4617    4763     387 
   Size:       1064     138    2654     374     146    1280

  Fragments arranged by size: 

                2654    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2057    2231    2449    2890    3719    4316    5103 
   Size:        393     425     174     218     441     829     597     787

Cuts at:   5103    5371    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     829     787     597     441     425     393     268
                 218     174
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2264    2264 
   Size:       5656
 
EcoRI G'AATT_C

Cuts at:   2965    3343    2965 
   Size:        378    5278
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2920    2920 
   Size:       5656
 
FspI TGC'GCA

Cuts at:   4162    4162 
   Size:       5656
 
HaeII r_GCGC'y

Cuts at:   1678    4803    1678 
   Size:       3125    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2013    2045    4297    4329    4371 
   Size:         32     135      32     441      32    2252      32      42

Cuts at:   4371    4403    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2252     441     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3000    3246     669 
   Size:        988     281    1062     246    3079

  Fragments arranged by size: 

                3079    1062     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2480     748 
   Size:       1732    3924
 
HpaI GTT'AAC

Cuts at:   3246    3246 
   Size:       5656
 
KpnI G_GTAC'C

Cuts at:   2981    2981 
   Size:       5656
 
MfeI C'AATT_G

Cuts at:   2498    3255    2498 
   Size:        757    4899
 
MluI A'CGCG_T

Cuts at:   5532    5532 
   Size:       5656
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2103    4770    4954    1305 
   Size:        798    2667     184    2007

  Fragments arranged by size: 

                2667    2007     798     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2196    2378    2426      10 
   Size:         54    1425     343     364     182      48    3240

  Fragments arranged by size: 

                3240    1425     364     343     182      54      48
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3614    3973    4132     518 
   Size:        648    2448     359     159    2042

  Fragments arranged by size: 

                2448    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2077    3072    3749    4897    5142    1606 
   Size:        471     995     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     995     677     471     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5104
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5656
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5656
 
NotI GC'GGCC_GC

Cuts at:   3103    3103 
   Size:       5656
 
NspI r_CATG'y

Cuts at:   1132    1229    3014    4559    1132 
   Size:         97    1785    1545    2229

  Fragments arranged by size: 

                2229    1785    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5656
 
PciI A'CATG_T

Cuts at:   4555    4555 
   Size:       5656
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5656
 
PfoI T'CCnGG_A

Cuts at:   2208    2208 
   Size:       5656
 
PmeI GTTT'AAAC

Cuts at:   2960    2960 
   Size:       5656
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3725    3757    5238    5270    3725 
   Size:         32    1481      32    4111

  Fragments arranged by size: 

                4111    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5656
 
PsiI TTA'TAA

Cuts at:   3226    3226 
   Size:       5656
 
PstI C_TGCA'G

Cuts at:    830    3008     830 
   Size:       2178    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5656
 
PvuII CAG'CTG

Cuts at:   2077    2077 
   Size:       5656
 
SalI G'TCGA_C

Cuts at:   1936    2998    1936 
   Size:       1062    4594
 
SbfI CC_TGCA'GG

Cuts at:   3008    3008 
   Size:       5656
 
ScaI AGT'ACT

Cuts at:   3904    5616    3904 
   Size:       1712    3944
 
SfcI C'TryA_G

Cuts at:    826    1045    3004    4139    4820    5011     826 
   Size:        219    1959    1135     681     191    1471

  Fragments arranged by size: 

                1959    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5656
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5656
 
SmaI CCC'GGG

Cuts at:   1872    2792    2983    1872 
   Size:        920     191    4545

  Fragments arranged by size: 

                4545     920     191
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3760    4661    4923     820 
   Size:        197     926    1817     901     262    1553

  Fragments arranged by size: 

                1817    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2287    3447     493 
   Size:       1794    1160    2702

  Fragments arranged by size: 

                2702    1794    1160
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5656
 
SphI G_CATG'C

Cuts at:   1132    3014    1132 
   Size:       1882    3774
 
SspI AAT'ATT

Cuts at:      5      52    3580       5 
   Size:         47    3528    2081

  Fragments arranged by size: 

                3528    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2903    1517 
   Size:       1386    4270
 
StyI C'CwwG_G

Cuts at:    513    1065    2191    2939    3095     513 
   Size:        552    1126     748     156    3074

  Fragments arranged by size: 

                3074    1126     748     552     156
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3683    3700    3853    4038    4483     325 
   Size:       1255    2103      17     153     185     445    1498

  Fragments arranged by size: 

                2103    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2181    2307    2330 
   Size:        274      80      33      51    1443     204     126      23

Cuts at:   2330    2799    3902    5614      96 
   Size:        469    1103    1712     138

  Fragments arranged by size: 

                1712    1443    1103     469     274     204     138     126
                  80      51      33      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5616    5648    5616 
   Size:         32    5624
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5656
 
XbaI T'CTAG_A

Cuts at:   2992    2992 
   Size:       5656
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5656
 
XmaI C'CCGG_G

Cuts at:   1870    2790    2981    1870 
   Size:        920     191    4545

  Fragments arranged by size: 

                4545     920     191
 
XmnI GAAnn'nnTTC

Cuts at:   3785    3785 
   Size:       5656
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3464     276 
   Size:         53      83     186     936    1930    2468

  Fragments arranged by size: 

                2468    1930     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI     BaeI     BbeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI EcoO109I
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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