################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MluI MroI MseI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 826 AAATTT [GA]^AATT[CT] AfaI 189 GTAC GT^AC ApoI 826 AAATTT [GA]^AATT[CT] BanII 908 GGGCTC G[GA]GC[CT]^C BcgI 473 GCAACTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 563 TGATCA T^GATCA BglI 358 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 936 CTGGAG CTGGAG(16/14) BsaAI 379 TACGTG [CT]AC^GT[GA] BseRI 915 GAGGAG GAGGAG(10/8) BsiEI 557 CGGCCG CG[GA][CT]^CG BsmBI 753 CGTCTC CGTCTC(1/5) Bsp1407I 188 TGTACA T^GTACA BspLU11I 782 ACATGT A^CATGT BsrFI 555 GCCGGC [GA]^CCGG[CT] BsrGI 188 TGTACA T^GTACA BssSI 442 CTCGTG CACGAG(-5/-1) BstAPI 338 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 555 GCCGGC [GA]^CCGG[CT] DraIII 339 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 557 CGGCCG C^GGCCG EclXI 557 CGGCCG C^GGCCG Eco52I 557 CGGCCG C^GGCCG FauI 518 GCGGG CCCGC(4/6) FbaI 563 TGATCA T^GATCA HincII 259 GTCAAC GT[CT]^[GA]AC HindII 259 GTCAAC GT[CT]^[GA]AC HinfI 31 GACTC G^A[ACGT]TC MfeI 505 CAATTG C^AATTG MslI 585 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 387 CAGCTG C[AC]G^C[GT]G MunI 505 CAATTG C^AATTG NaeI 555 GCCGGC GCC^GGC NcoI 362 CCATGG C^CATGG NgoMIV 555 GCCGGC G^CCGGC NspI 782 ACATGT [GA]CATG^[CT] PleI 31 GACTC GAGTC(4/5) PstI 790 CTGCAG CTGCA^G PvuII 387 CAGCTG CAG^CTG RsaI 189 GTAC GT^AC SfcI 790 CTGCAG C^T[GA][CT]AG SfiI 357 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 188 TGTACA T^GTACA StuI 719 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 730 ACGTGT A^C[GA][CT]GT 782 ACATGT AspHI 425 GTGCTC G[AT]GC[AT]^C 685 GTGCTC AvaI 158 CCCGGG C^[CT]CG[GA]G 677 CCCGGG BamHI 165 GGATCC G^GATCC 692 GGATCC BanI 552 GGTGCC G^G[CT][GA]CC 636 GGTGCC BbsI 279 GAAGAC GAAGAC(2/6) 319 GTCTTC BpuAI 279 GAAGAC GAAGAC(2/6) 319 GTCTTC BsiHKAI 425 GTGCTC G[AT]GC[AT]^C 685 GTGCTC BsmAI 918 GAGAC GTCTC(1/5) 754 GTCTC BsoBI 158 CCCGGG C^[CT]CG[GA]G 677 CCCGGG BstXI 143 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 362 CCATGGCCCTGG DsaI 73 CCGTGG C^C[GA][CT]GG 362 CCATGG EarI 760 CTCTTC CTCTTC(1/4) 838 CTCTTC EcoT14I 362 CCATGG C^C[AT][AT]GG 704 CCAAGG Ksp632I 760 CTCTTC CTCTTC(1/4) 838 CTCTTC MaeIII 8 GTGAC ^GT[ACGT]AC 203 GTCAC PflMI 324 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 929 CCAGGATCTGG SmaI 158 CCCGGG CCC^GGG 677 CCCGGG StyI 362 CCATGG C^C[AT][AT]GG 704 CCAAGG Tsp45I 8 GTGAC ^GT[GC]AC 203 GTCAC Tsp509I 506 AATT ^AATT 827 AATT Van91I 324 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 929 CCAGGATCTGG XmaCI 158 CCCGGG C^CCGGG 677 CCCGGG XmaI 158 CCCGGG C^CCGGG 677 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BalI BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MboI MboII MflI MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI XhoII