(Circular) (Six-base) MAPSORT of: pFN21AE6038.seq Check: 4466 from: 1 to: 5656 pFN21AE6038 5656 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2482 2521 2482 Size: 39 5617 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3719 5103 5371 1632 Size: 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2735 2787 4555 5532 1203 Size: 1532 52 1768 977 1327 Fragments arranged by size: 1768 1532 1327 977 52 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2420 1184 Size: 1236 4420 AlwNI CAG_nnn'CTG Cuts at: 2260 2536 2940 3399 4971 2260 Size: 276 404 459 1572 2945 Fragments arranged by size: 2945 1572 459 404 276 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2831 2965 3191 3343 5452 1493 Size: 1338 134 226 152 2109 1697 Fragments arranged by size: 2109 1697 1338 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2163 2682 2981 1870 Size: 73 220 519 299 4545 Fragments arranged by size: 4545 519 299 220 73 BamHI G'GATC_C Cuts at: 2170 2697 2986 3353 2170 Size: 527 289 367 4473 Fragments arranged by size: 4473 527 367 289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2557 2641 2977 4432 618 Size: 325 217 627 770 84 336 1455 1842 Fragments arranged by size: 1842 1455 770 627 336 325 217 84 BanII G_rGCy'C Cuts at: 1391 2917 2975 1391 Size: 1526 58 4072 Fragments arranged by size: 4072 1526 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2291 2317 928 Size: 971 392 26 4267 Fragments arranged by size: 4267 971 392 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2467 2501 3847 3881 1903 Size: 34 530 34 1346 34 3678 Fragments arranged by size: 3678 1346 530 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 2568 1548 Size: 1020 4636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2369 4267 136 Size: 107 122 71 631 605 697 1898 1525 Fragments arranged by size: 1898 1525 697 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2646 3717 4873 1790 Size: 856 1071 1156 2573 Fragments arranged by size: 2573 1156 1071 856 BmgBI CAC'GTC Cuts at: 1318 2736 1318 Size: 1418 4238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2962 4316 1239 Size: 1723 1354 2579 Fragments arranged by size: 2579 1723 1354 Bpu10I CC'TnA_GC Cuts at: 2693 2693 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2386 3447 493 Size: 711 1182 1061 2702 Fragments arranged by size: 2702 1182 1061 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2935 2965 1178 Size: 30 165 30 1532 30 3869 Fragments arranged by size: 3869 1532 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2935 1186 Size: 1749 3907 BseYI C'CCAG_C Cuts at: 2335 4859 5355 2335 Size: 2524 496 2636 Fragments arranged by size: 2636 2524 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 2390 2510 1163 Size: 573 554 100 120 4309 Fragments arranged by size: 4309 573 554 120 100 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2565 3106 3867 4895 1540 Size: 275 186 564 541 761 1028 2301 Fragments arranged by size: 2301 1028 761 564 541 275 186 BsiHKAI G_wGCw'C Cuts at: 2434 2694 3717 3802 4873 2434 Size: 260 1023 85 1071 3217 Fragments arranged by size: 3217 1071 1023 260 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 BsmBI CGTCTCn'nnnn_ Cuts at: 2764 2764 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2434 2646 2694 2917 2975 3717 3802 Size: 399 644 212 48 223 58 742 85 Cuts at: 3802 4873 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 644 399 223 212 85 58 48 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 2521 2785 3011 844 Size: 1638 39 264 226 3489 Fragments arranged by size: 3489 1638 264 226 39 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2560 4300 4487 1700 Size: 110 750 1740 187 2869 Fragments arranged by size: 2869 1740 750 187 110 BsrGI T'GTAC_A Cuts at: 96 2193 96 Size: 2097 3559 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 2447 3716 4728 2447 Size: 1269 1012 3375 Fragments arranged by size: 3375 1269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2349 794 Size: 1555 4101 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2374 2155 Size: 219 5437 BstYI r'GATC_y Cuts at: 1972 2170 2697 2937 2986 3353 3738 3755 5207 Size: 198 527 240 49 367 385 17 1452 Cuts at: 5207 5293 5305 5651 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 527 385 367 346 240 198 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2078 2367 513 Size: 552 1013 289 3802 Fragments arranged by size: 3802 1013 552 289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2694 3172 3966 3986 2694 Size: 478 794 20 4364 Fragments arranged by size: 4364 794 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DraIII CAC_nnn'GTG Cuts at: 2349 2349 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2361 2388 Size: 54 1425 50 275 18 297 234 27 Cuts at: 2388 2562 2931 3103 3992 5621 8 Size: 174 369 172 889 1629 43 Fragments arranged by size: 1629 1425 889 369 297 275 234 174 172 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2562 3103 1537 Size: 275 750 541 4090 Fragments arranged by size: 4090 750 541 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2771 2849 3586 1353 Size: 1418 78 737 3423 Fragments arranged by size: 3423 1418 737 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2962 3719 4316 5103 5371 1239 Size: 393 1330 757 597 787 268 1524 Fragments arranged by size: 1524 1330 787 757 597 393 268 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2236 2268 4297 4329 4371 Size: 32 135 32 664 32 2029 32 42 Cuts at: 4371 4403 1373 Size: 32 2626 Fragments arranged by size: 2626 2029 664 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 3000 3246 669 Size: 988 281 328 734 246 3079 Fragments arranged by size: 3079 988 734 328 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 2510 3255 2510 Size: 745 4911 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 2390 2933 10 Size: 54 1425 343 297 261 543 2733 Fragments arranged by size: 2733 1425 543 343 297 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2594 3614 3973 4132 518 Size: 648 1428 1020 359 159 2042 Fragments arranged by size: 2042 1428 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2394 3072 3749 4897 5142 1606 Size: 788 678 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 678 677 245 NaeI GCC'GGC Cuts at: 2562 2562 Size: 5656 NcoI C'CATG_G Cuts at: 513 1065 2367 513 Size: 552 1302 3802 Fragments arranged by size: 3802 1302 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NgoMIV G'CCGG_C Cuts at: 2560 2560 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2791 3014 4559 1132 Size: 97 1562 223 1545 2229 Fragments arranged by size: 2229 1562 1545 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 2787 4555 2787 Size: 1768 3888 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 2940 1108 Size: 1227 605 3824 Fragments arranged by size: 3824 1227 605 PfoI T'CCnGG_A Cuts at: 2220 2220 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2079 2111 3725 3757 5238 5270 2079 Size: 32 1614 32 1481 32 2465 Fragments arranged by size: 2465 1614 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 2799 3008 830 Size: 1969 209 3478 Fragments arranged by size: 3478 1969 209 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2394 2394 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2795 3004 4139 4820 5011 826 Size: 219 1750 209 1135 681 191 1471 Fragments arranged by size: 1750 1471 1135 681 219 209 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2369 2369 Size: 5656 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2165 2684 2983 1872 Size: 293 519 299 4545 Fragments arranged by size: 4545 519 299 293 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SrfI GCCC'GGGC Cuts at: 2684 2684 Size: 5656 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 2726 1517 Size: 1209 4447 StyI C'CwwG_G Cuts at: 513 1065 2367 2709 3095 513 Size: 552 1302 342 386 3074 Fragments arranged by size: 3074 1302 552 386 342 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 3683 3700 3853 4038 4483 325 Size: 1255 471 1632 17 153 185 445 1498 Fragments arranged by size: 1632 1498 1255 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 3902 5614 Size: 274 80 33 51 1443 216 1709 1712 Cuts at: 5614 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2857 2889 2935 2967 5616 5648 2857 Size: 32 46 32 2649 32 2865 Fragments arranged by size: 2865 2649 46 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2163 2682 2981 1870 Size: 293 519 299 4545 Fragments arranged by size: 4545 519 299 293 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SwaI XcmI