(Circular) (Six-base) MAPSORT of: pFN21AE6031.seq Check: 1536 from: 1 to: 5653 pFN21AE6031 5653 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2914 2996 1937 Size: 977 82 4594 Fragments arranged by size: 4594 977 82 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 2952 3780 4153 1563 Size: 1389 828 373 3063 Fragments arranged by size: 3063 1389 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2715 3716 5100 5368 1632 Size: 1083 1001 1384 268 1917 Fragments arranged by size: 1917 1384 1083 1001 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 2577 2778 2917 4552 5529 1203 Size: 1374 201 139 1635 977 1327 Fragments arranged by size: 1635 1374 1327 977 201 139 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 2921 4382 2921 Size: 1461 4192 AleI CACnn'nnGTG Cuts at: 1166 2027 2629 1166 Size: 861 602 4190 Fragments arranged by size: 4190 861 602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2162 2292 2693 3396 4968 2162 Size: 130 401 703 1572 2847 Fragments arranged by size: 2847 1572 703 401 130 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2036 2128 2962 3188 3340 5449 1493 Size: 543 92 834 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 543 226 152 92 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2974 4429 618 Size: 325 217 627 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 627 325 217 BanII G_rGCy'C Cuts at: 1391 2169 2972 1391 Size: 778 803 4072 Fragments arranged by size: 4072 803 778 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2596 928 Size: 971 697 3985 Fragments arranged by size: 3985 971 697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BfrBI ATG'CAT Cuts at: 2216 2216 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4264 136 Size: 107 122 71 631 605 2592 1525 Fragments arranged by size: 2592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2786 3714 4870 1790 Size: 996 928 1156 2573 Fragments arranged by size: 2573 1156 996 928 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4313 1239 Size: 3074 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2049 3778 4643 4941 2049 Size: 1729 865 298 2761 Fragments arranged by size: 2761 1729 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2343 1186 Size: 1157 4496 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 1163 Size: 573 642 4438 Fragments arranged by size: 4438 642 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3714 3799 4870 3714 Size: 85 1071 4497 Fragments arranged by size: 4497 1071 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2786 2972 3714 3799 4870 1391 Size: 399 379 617 186 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 617 399 379 186 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 2409 3542 5272 2409 Size: 1133 1730 2790 Fragments arranged by size: 2790 1730 1133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2770 3008 844 Size: 1926 238 3489 Fragments arranged by size: 3489 1926 238 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 4148 4322 65 Size: 1183 1191 1709 174 1396 Fragments arranged by size: 1709 1396 1191 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 4297 4484 1700 Size: 110 575 1912 187 2869 Fragments arranged by size: 2869 1912 575 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 2730 3713 4725 2730 Size: 983 1012 3658 Fragments arranged by size: 3658 1012 983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2359 487 Size: 683 56 1133 3781 Fragments arranged by size: 3781 1133 683 56 BtsI GCAGTG_nn' Cuts at: 3169 3963 3983 3169 Size: 794 20 4839 Fragments arranged by size: 4839 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DraIII CAC_nnn'GTG Cuts at: 2833 2833 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618 Size: 54 1425 50 275 18 1270 889 1629 Cuts at: 5618 8 Size: 43 Fragments arranged by size: 1629 1425 1270 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2894 3583 1353 Size: 1541 689 3423 Fragments arranged by size: 3423 1541 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2715 3716 4313 5100 5368 1239 Size: 393 1083 1001 597 787 268 1524 Fragments arranged by size: 1524 1083 1001 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2793 2793 Size: 5653 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2246 2278 2280 Size: 32 135 32 475 32 167 32 2 Cuts at: 2280 2312 2428 2460 2541 2573 2751 2783 2803 Size: 32 116 32 81 32 178 32 20 Cuts at: 2803 2835 2907 2939 4294 4326 4368 4400 1373 Size: 32 72 32 1355 32 42 32 2626 Fragments arranged by size: 2626 1355 475 178 167 135 116 81 72 42 32 32 32 32 32 32 32 32 32 32 32 32 20 2 HincII GTy'rAC Cuts at: 669 1657 1938 2915 2997 3243 669 Size: 988 281 977 82 246 3079 Fragments arranged by size: 3079 988 977 281 246 82 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2572 4767 4951 1305 Size: 1267 2195 184 2007 Fragments arranged by size: 2195 2007 1267 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3831 Fragments arranged by size: 3831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2576 2582 2629 2777 3611 3970 Size: 648 861 549 6 47 148 834 359 Cuts at: 3970 4129 518 Size: 159 2042 Fragments arranged by size: 2042 861 834 648 549 359 159 148 47 6 MspA1I CmG'CkG Cuts at: 1606 2524 2804 3069 3746 4894 5139 1606 Size: 918 280 265 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 677 280 265 245 NaeI GCC'GGC Cuts at: 2387 2387 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NgoMIV G'CCGG_C Cuts at: 2385 2385 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NsiI A_TGCA'T Cuts at: 2218 2218 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 2581 2724 2782 2921 3011 4556 1132 Size: 97 1352 143 58 139 90 1545 2229 Fragments arranged by size: 2229 1545 1352 143 139 97 90 58 PasI CC'CwG_GG Cuts at: 1348 2162 1348 Size: 814 4839 PciI A'CATG_T Cuts at: 2577 2778 2917 4552 2577 Size: 201 139 1635 3678 Fragments arranged by size: 3678 1635 201 139 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 2942 3223 2942 Size: 281 5372 PstI C_TGCA'G Cuts at: 830 3005 830 Size: 2175 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2524 2804 2524 Size: 280 5373 SalI G'TCGA_C Cuts at: 1936 2913 2995 1936 Size: 977 82 4594 Fragments arranged by size: 4594 977 82 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2284 3001 4136 4817 5008 826 Size: 219 1239 717 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 717 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2064 3757 4658 4920 820 Size: 197 926 121 1693 901 262 1553 Fragments arranged by size: 1693 1553 926 901 262 197 121 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2047 2323 3092 513 Size: 552 982 276 769 3074 Fragments arranged by size: 3074 982 769 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3680 3697 3850 4035 4480 325 Size: 1255 2100 17 153 185 445 1498 Fragments arranged by size: 2100 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3899 5611 96 Size: 274 80 33 51 1443 1922 1712 138 Fragments arranged by size: 1922 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 2059 1316 Size: 743 4910 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2479 2248 Size: 231 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI