(Linear) (Six-base) MAPSORT of: KIEE6031.seq Check: 2782 from: 1 to: 948 KIEE6031.orf With 182 enzymes: SgfI * December 5, 2014 13:20 .. AccI GT'mk_AC Cuts at: 0 909 948 Size: 909 39 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 710 948 Size: 710 238 AflIII A'CryG_T Cuts at: 0 572 773 912 948 Size: 572 201 139 36 Fragments arranged by size: 572 201 139 36 AhdI GACnn_n'nnGTC Cuts at: 0 916 948 Size: 916 32 AleI CACnn'nnGTG Cuts at: 0 22 624 948 Size: 22 602 324 Fragments arranged by size: 602 324 22 AlwNI CAG_nnn'CTG Cuts at: 0 157 287 688 948 Size: 157 130 401 260 Fragments arranged by size: 401 260 157 130 ApoI r'AATT_y Cuts at: 0 31 123 948 Size: 31 92 825 Fragments arranged by size: 825 92 31 BanII G_rGCy'C Cuts at: 0 164 948 Size: 164 784 BbsI GAAGACnn'nnnn_ Cuts at: 0 591 948 Size: 591 357 BfrBI ATG'CAT Cuts at: 0 211 948 Size: 211 737 Bme1580I G_kGCm'C Cuts at: 0 781 948 Size: 781 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 948 Size: 44 904 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 338 948 Size: 338 610 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 948 Size: 373 575 Bsp1286I G_dGCh'C Cuts at: 0 164 781 948 Size: 164 617 167 Fragments arranged by size: 617 167 164 BspHI T'CATG_A Cuts at: 0 404 948 Size: 404 544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 765 948 Size: 765 183 BsrDI GCAATG_nn' Cuts at: 0 434 948 Size: 434 514 BsrFI r'CCGG_y Cuts at: 0 380 948 Size: 380 568 BssSI C'ACGA_G Cuts at: 0 725 948 Size: 725 223 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 948 Size: 243 705 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 354 948 Size: 354 594 DraIII CAC_nnn'GTG Cuts at: 0 828 948 Size: 828 120 EarI CTCTTCn'nnn_ Cuts at: 0 889 948 Size: 889 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 710 948 Size: 710 238 EcoO109I rG'GnC_Cy Cuts at: 0 788 948 Size: 788 160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 42 74 241 273 275 307 423 455 Size: 42 32 167 32 2 32 116 32 Cuts at: 455 536 568 746 778 798 830 902 934 Size: 81 32 178 32 20 32 72 32 Cuts at: 934 948 Size: 14 Fragments arranged by size: 178 167 116 81 72 42 32 32 32 32 32 32 32 32 20 14 2 HincII GTy'rAC Cuts at: 0 910 948 Size: 910 38 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 567 948 Size: 567 381 MslI CAynn'nnrTG Cuts at: 0 22 571 577 624 772 948 Size: 22 549 6 47 148 176 Fragments arranged by size: 549 176 148 47 22 6 MspA1I CmG'CkG Cuts at: 0 519 799 948 Size: 519 280 149 Fragments arranged by size: 519 280 149 NaeI GCC'GGC Cuts at: 0 382 948 Size: 382 566 NgoMIV G'CCGG_C Cuts at: 0 380 948 Size: 380 568 NsiI A_TGCA'T Cuts at: 0 213 948 Size: 213 735 NspI r_CATG'y Cuts at: 0 576 719 777 916 948 Size: 576 143 58 139 32 Fragments arranged by size: 576 143 139 58 32 PasI CC'CwG_GG Cuts at: 0 157 948 Size: 157 791 PciI A'CATG_T Cuts at: 0 572 773 912 948 Size: 572 201 139 36 Fragments arranged by size: 572 201 139 36 PfoI T'CCnGG_A Cuts at: 0 203 948 Size: 203 745 PsiI TTA'TAA Cuts at: 0 937 948 Size: 937 11 PvuII CAG'CTG Cuts at: 0 519 799 948 Size: 519 280 149 Fragments arranged by size: 519 280 149 SalI G'TCGA_C Cuts at: 0 908 948 Size: 908 40 SfcI C'TryA_G Cuts at: 0 279 948 Size: 279 669 SmlI C'TyrA_G Cuts at: 0 59 948 Size: 59 889 StyI C'CwwG_G Cuts at: 0 42 318 948 Size: 42 276 630 Fragments arranged by size: 630 276 42 Tth111I GACn'n_nGTC Cuts at: 0 54 948 Size: 54 894 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 243 474 948 Size: 243 231 474 Fragments arranged by size: 474 243 231 Enzymes that do cut: AccI AcuI AflIII AhdI AleI AlwNI ApoI BanII BbsI BfrBI Bme1580I BpuEI BseRI BsgI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstXI BtgZI DraIII EarI Eco57MI EcoO109I Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PciI PfoI PsiI PvuII SalI SfcI SmlI StyI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI PacI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XhoI XmaI XmnI ZraI