################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 205 GTAGAC GT^[AC][GT]AC AcsI 31 GAATTC [GA]^AATT[CT] AfaI 774 GTAC GT^AC AluI 625 AGCT AG^CT AlwNI 475 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTC [GA]^AATT[CT] Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 149 GGACC G^G[AT]CC BamHI 680 GGATCC G^GATCC BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG BsgI 244 CTGCAC GTGCAG(16/14) BsiYI 78 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 78 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 6 GTCCC GGGAC(10/14) BspLU11I 446 ACATGT A^CATGT BsrDI 592 GCAATG GCAATG(2/0) BstAPI 122 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 190 GGTCACC G^GT[ACGT]ACC BstPI 190 GGTCACC G^GT[ACGT]ACC CfoI 536 GCGC GCG^C Cfr13I 149 GGACC G^G[ACGT]CC DraIII 294 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 571 GACACCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 190 GGTCACC G^GT[ACGT]ACC EcoRI 31 GAATTC G^AATTC EcoT14I 186 CCTTGG C^C[AT][AT]GG HhaI 536 GCGC GCG^C HinP1I 536 GCGC G^CGC HinfI 808 GAGTC G^A[ACGT]TC HphI 192 TCACC GGTGA(8/7) MseI 461 TTAA T^TAA MslI 18 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PleI 808 GAGTC GAGTC(4/5) PshAI 735 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 774 GTAC GT^AC Sau96I 149 GGACC G^G[ACGT]CC SmlI 621 CTCAAG C^T[CT][GA]AG SnaBI 775 TACGTA TAC^GTA StuI 781 AGGCCT AGG^CCT StyI 186 CCTTGG C^C[AT][AT]GG Tru9I 461 TTAA T^TAA XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 446 ACATGT A^C[GA][CT]GT 718 ACGTGT BanII 466 GGGCTC G[GA]GC[CT]^C 843 GAGCCC BbsI 267 GAAGAC GAAGAC(2/6) 302 GTCTTC BclI 143 TGATCA T^GATCA 323 TGATCA BfaI 67 CTAG C^TAG 319 CTAG BmyI 466 GGGCTC G[AGT]GC[ACT]^C 843 GAGCCC BpuAI 267 GAAGAC GAAGAC(2/6) 302 GTCTTC BsaAI 717 TACGTG [CT]AC^GT[GA] 775 TACGTA BseRI 545 CTCCTC GAGGAG(10/8) 805 GAGGAG Bsp1286I 466 GGGCTC G[AGT]GC[ACT]^C 843 GAGCCC BsrI 114 ACTGG ACTGG(1/-1) 61 CCAGT BssKI 93 CCTGG ^CC[ACGT]GG 401 CCAGG BstF5I 286 GGATG GGATG(2/0) 404 GGATG BstNI 93 CCTGG CC^[AT]GG 401 CCAGG BstYI 74 AGATCC [GA]^GATC[CT] 680 GGATCC EcoRII 93 CCTGG ^CC[AT]GG 401 CCAGG FbaI 143 TGATCA T^GATCA 323 TGATCA FokI 286 GGATG GGATG(9/13) 404 GGATG HaeIII 561 GGCC GG^CC 782 GGCC MaeI 67 CTAG C^TAG 319 CTAG MaeII 718 ACGT A^CGT 776 ACGT MflI 74 AGATCC [GA]^GATC[CT] 680 GGATCC MvaI 93 CCTGG CC^[AT]GG 401 CCAGG MwoI 122 GCAACCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 346 GCTTCAATGGC NspI 446 ACATGT [GA]CATG^[CT] 770 GCATGT ScrFI 93 CCTGG CC^[ACGT]GG 401 CCAGG SfaNI 287 GATGC GCATC(5/9) 422 GCATC SfcI 800 CTGTAG C^T[GA][CT]AG 867 CTATAG XhoII 74 AGATCC [GA]^GATC[CT] 680 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AlwI BbvI Cac8I DdeI DpnII EarI Fnu4HI ItaI Ksp632I MaeIII MboI MboII MnlI NdeII NlaIII NlaIV Sau3AI TseI Tsp45I Tsp509I TspRI