Restriction map for
pFN21AE6025
(Circular) (Six-base) MAPSORT of: pFN21AE6025.seq Check: 1003 from: 1 to: 5650
pFN21AE6025 5650 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2175 2175
Size: 5650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 2993 1937
Size: 274 782 4594
Fragments arranged by size:
4594 782 274
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2665 2718 3713 5097 5365 1632
Size: 1033 53 995 1384 268 1917
Fragments arranged by size:
1917 1384 1033 995 268 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2451 2723 4549 5526 1203
Size: 1248 272 1826 977 1327
Fragments arranged by size:
1826 1327 1248 977 272
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 2027 1166
Size: 861 4789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 2408 1184
Size: 975 249 4426
Fragments arranged by size:
4426 975 249
AlwNI CAG_nnn'CTG
Cuts at: 2485 3393 4965 2485
Size: 908 1572 3170
Fragments arranged by size:
3170 1572 908
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2036 2959 3185 3337 5446 1493
Size: 543 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2685 2980 3347 2685
Size: 295 367 4988
Fragments arranged by size:
4988 367 295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2969 1391
Size: 1578 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 2300 928
Size: 971 380 21 4278
Fragments arranged by size:
4278 971 380 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 2148 2328 1548
Size: 600 180 4870
Fragments arranged by size:
4870 600 180
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3381 4339
Size: 416 53 83 399 24 643 1321 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 1321 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4310 1239
Size: 3071 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2611 3775 4640 4938 2611
Size: 1164 865 298 3323
Fragments arranged by size:
3323 1164 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2724 2782 3441 493
Size: 711 1520 58 659 2702
Fragments arranged by size:
2702 1520 711 659 58
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2541 2825 1186
Size: 1355 284 4011
Fragments arranged by size:
4011 1355 284
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 1163
Size: 573 498 4579
Fragments arranged by size:
4579 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2969 3711 3796 4867 1391
Size: 399 1179 742 85 1071 2174
Fragments arranged by size:
2174 1179 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 3005 844
Size: 1331 830 3489
Fragments arranged by size:
3489 1331 830
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2604 4145 4319 65
Size: 1183 1356 1541 174 1396
Fragments arranged by size:
1541 1396 1356 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2133 794
Size: 1339 4311
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2079 2685 2980 3347 3732 3749 5201 5287
Size: 107 606 295 367 385 17 1452 86
Cuts at: 5287 5299 5645 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 606 385 367 346 295 107
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5098
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2302 487
Size: 683 56 1076 3835
Fragments arranged by size:
3835 1076 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 3166 3960 3980 2483
Size: 683 794 20 4153
Fragments arranged by size:
4153 794 683 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DraIII CAC_nnn'GTG
Cuts at: 2304 2304
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 4657 809
Size: 507 1266 2075 1802
Fragments arranged by size:
2075 1802 1266 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 3097 3986
Size: 54 1425 50 275 18 364 903 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 903 889 364 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2642 2774 3580 1353
Size: 965 324 132 806 3423
Fragments arranged by size:
3423 965 806 324 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2665 2718 3713 4310 5097 5365 1239
Size: 393 1033 53 995 597 787 268 1524
Fragments arranged by size:
1524 1033 995 787 597 393 268 53
EcoRI G'AATT_C
Cuts at: 2036 2959 3337 2036
Size: 923 378 4349
Fragments arranged by size:
4349 923 378
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373
Size: 32 135 32 2719 32 42 32 2626
Fragments arranged by size:
2719 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4764 4948 1305
Size: 3459 184 2007
Fragments arranged by size:
3459 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2196 10
Size: 54 1425 343 364 3464
Fragments arranged by size:
3464 1425 364 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 3608 3967 4126 518
Size: 648 861 1581 359 159 2042
Fragments arranged by size:
2042 1581 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3066 3743 4891 5136 1606
Size: 1460 677 1148 245 2120
Fragments arranged by size:
2120 1460 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2455 2779 3008 4553 1132
Size: 97 1226 324 229 1545 2229
Fragments arranged by size:
2229 1545 1226 324 229 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 2451 4549 2451
Size: 2098 3552
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PfoI T'CCnGG_A
Cuts at: 2405 2405
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2744 1613
Size: 1131 4519
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2830 3002 830
Size: 2000 172 3478
Fragments arranged by size:
3478 2000 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2805 2826 2872 2998 4133 4814 5005
Size: 219 1760 21 46 126 1135 681 191
Cuts at: 5005 826
Size: 1471
Fragments arranged by size:
1760 1471 1135 681 219 191 126 46
21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2626 3754 4655 4917 820
Size: 197 926 683 1128 901 262 1553
Fragments arranged by size:
1553 1128 926 901 683 262 197
SnaBI TAC'GTA
Cuts at: 493 2782 3441 493
Size: 2289 659 2702
Fragments arranged by size:
2702 2289 659
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2788 1517
Size: 1271 4379
StyI C'CwwG_G
Cuts at: 513 1065 2191 3089 513
Size: 552 1126 898 3074
Fragments arranged by size:
3074 1126 898 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 3677 3694 3847 4032 4477 325
Size: 1255 611 1486 17 153 185 445 1498
Fragments arranged by size:
1498 1486 1255 611 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3896 5608 96
Size: 274 80 33 51 1443 1919 1712 138
Fragments arranged by size:
1919 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3779 2040
Size: 1739 3911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI