(Circular) (Six-base) MAPSORT of: pFN21AE6024.seq Check: 5770 from: 1 to: 5650 pFN21AE6024 5650 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2060 2668 2930 3713 5097 5365 1632 Size: 428 608 262 783 1384 268 1917 Fragments arranged by size: 1917 1384 783 608 428 268 262 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 2585 2846 1166 Size: 1419 261 3970 Fragments arranged by size: 3970 1419 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2840 1184 Size: 1656 3994 AlwNI CAG_nnn'CTG Cuts at: 2615 3393 4965 2615 Size: 778 1572 3300 Fragments arranged by size: 3300 1572 778 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2036 2959 3185 3337 5446 1493 Size: 543 923 226 152 2109 1697 Fragments arranged by size: 2109 1697 923 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 BamHI G'GATC_C Cuts at: 2654 2980 3347 2654 Size: 326 367 4957 Fragments arranged by size: 4957 367 326 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 4426 618 Size: 325 217 627 1184 1455 1842 Fragments arranged by size: 1842 1455 1184 627 325 217 BanII G_rGCy'C Cuts at: 1391 2939 2969 1391 Size: 1548 30 4072 Fragments arranged by size: 4072 1548 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4679 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 5650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 2262 1548 Size: 714 4936 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 2438 4261 Size: 107 122 71 631 605 688 78 1823 Cuts at: 4261 136 Size: 1525 Fragments arranged by size: 1823 1525 688 631 605 122 107 78 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2244 2434 3711 4867 1790 Size: 454 190 1277 1156 2573 Fragments arranged by size: 2573 1277 1156 454 190 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 3381 4339 Size: 416 53 83 399 24 982 982 958 Cuts at: 4339 442 Size: 1753 Fragments arranged by size: 1753 982 982 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2618 4310 1239 Size: 995 384 1692 2579 Fragments arranged by size: 2579 1692 995 384 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2866 3775 4640 4938 2866 Size: 909 865 298 3578 Fragments arranged by size: 3578 909 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2377 2781 3441 493 Size: 711 1173 404 660 2702 Fragments arranged by size: 2702 1173 711 660 404 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2076 2106 1178 Size: 30 165 30 673 30 4722 Fragments arranged by size: 4722 673 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5650 BseYI C'CCAG_C Cuts at: 2251 2433 4853 5349 2251 Size: 182 2420 496 2552 Fragments arranged by size: 2552 2420 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2151 2889 1163 Size: 573 415 738 3924 Fragments arranged by size: 3924 738 573 415 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3711 3796 4867 3711 Size: 85 1071 4494 Fragments arranged by size: 4494 1071 85 BsmI GAATG_Cn' Cuts at: 2717 3161 3254 2717 Size: 444 93 5113 Fragments arranged by size: 5113 444 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2434 2939 2969 3711 3796 4867 Size: 399 454 190 505 30 742 85 1071 Cuts at: 4867 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 505 454 399 190 85 30 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3005 844 Size: 2161 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 4145 4319 65 Size: 1183 889 2008 174 1396 Fragments arranged by size: 2008 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 2045 3710 4722 2045 Size: 1665 1012 2973 Fragments arranged by size: 2973 1665 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2251 2113 Size: 138 5512 BstYI r'GATC_y Cuts at: 1972 2654 2980 3347 3732 3749 5201 5287 5299 Size: 682 326 367 385 17 1452 86 12 Cuts at: 5299 5645 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 682 385 367 346 326 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2358 513 Size: 552 1293 3805 Fragments arranged by size: 3805 1293 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 2766 3166 3960 3980 2766 Size: 400 794 20 4436 Fragments arranged by size: 4436 794 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 2379 2472 Size: 54 1425 50 275 18 522 27 93 Cuts at: 2472 3097 3986 5615 8 Size: 625 889 1629 43 Fragments arranged by size: 1629 1425 889 625 522 275 93 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 2812 2842 3580 1353 Size: 1409 50 30 738 3423 Fragments arranged by size: 3423 1409 738 50 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2722 4237 4611 4757 387 Size: 1064 138 1133 1515 374 146 1280 Fragments arranged by size: 1515 1280 1133 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2234 2618 2668 2930 3713 4310 Size: 393 428 174 384 50 262 783 597 Cuts at: 4310 5097 5365 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 783 597 428 393 384 268 262 174 50 EcoO109I rG'GnC_Cy Cuts at: 2256 2928 2256 Size: 672 4978 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2646 2678 4291 4323 4365 Size: 32 135 32 1074 32 1613 32 42 Cuts at: 4365 4397 1373 Size: 32 2626 Fragments arranged by size: 2626 1613 1074 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 2025 3249 2025 Size: 1224 4426 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2812 4764 4948 1305 Size: 1507 1952 184 2007 Fragments arranged by size: 2007 1952 1507 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2354 2381 2474 10 Size: 54 1425 343 522 27 93 3186 Fragments arranged by size: 3186 1425 522 343 93 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2585 2846 3608 3967 4126 518 Size: 648 1140 279 261 762 359 159 2042 Fragments arranged by size: 2042 1140 762 648 359 279 261 159 MspA1I CmG'CkG Cuts at: 1606 2437 2444 2699 3066 3743 4891 5136 1606 Size: 831 7 255 367 677 1148 245 2120 Fragments arranged by size: 2120 1148 831 677 367 255 245 7 NcoI C'CATG_G Cuts at: 513 1065 2358 513 Size: 552 1293 3805 Fragments arranged by size: 3805 1293 552 NdeI CA'TA_TG Cuts at: 387 2365 387 Size: 1978 3672 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 2932 1348 Size: 1584 4066 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2743 1108 Size: 902 733 4015 Fragments arranged by size: 4015 902 733 PfoI T'CCnGG_A Cuts at: 2211 2211 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2256 2928 2256 Size: 672 4978 PshAI GACnn'nnGTC Cuts at: 1613 2027 1613 Size: 414 5236 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 3002 830 Size: 2172 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2437 2444 2699 2437 Size: 7 255 5388 Fragments arranged by size: 5388 255 7 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SanDI GG'GwC_CC Cuts at: 2256 2256 Size: 5650 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2154 2761 2875 2998 4133 4814 5005 Size: 219 1109 607 114 123 1135 681 191 Cuts at: 5005 826 Size: 1471 Fragments arranged by size: 1471 1135 1109 681 607 219 191 123 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2881 3754 4655 4917 820 Size: 197 926 938 873 901 262 1553 Fragments arranged by size: 1553 938 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 2947 152 Size: 2795 2855 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5650 StyI C'CwwG_G Cuts at: 513 1065 2290 2358 3089 513 Size: 552 1225 68 731 3074 Fragments arranged by size: 3074 1225 731 552 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3677 3694 3847 4032 4477 325 Size: 1255 2097 17 153 185 445 1498 Fragments arranged by size: 2097 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3896 5608 Size: 274 80 33 51 1443 207 1712 1712 Cuts at: 5608 96 Size: 138 Fragments arranged by size: 1712 1712 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 2040 2534 3779 2040 Size: 494 1245 3911 Fragments arranged by size: 3911 1245 494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI