################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflIII 644 ACGTGT A^C[GA][CT]GT AlwI 288 GATCC GGATC(4/5) AlwNI 408 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 511 ATTAAT AT^TAAT AsnI 511 ATTAAT AT^TAAT AvaII 638 GGTCC G^G[AT]CC AvrII 249 CCTAGG C^CTAGG BanII 615 GAGCTC G[GA]GC[CT]^C BciVI 671 GTATCC GTATCC(6/5) BclI 147 TGATCA T^GATCA BfaI 250 CTAG C^TAG BlnI 249 CCTAGG C^CTAGG BsaAI 643 TACGTG [CT]AC^GT[GA] BsaI 771 GAGACC GGTCTC(1/5) BsgI 382 CTGCAC GTGCAG(16/14) BsmFI 316 GGGAC GGGAC(10/14) BsmI 625 GCATTC GAATGC(1/-1) Bsp1407I 647 TGTACA T^GTACA BsrGI 647 TGTACA T^GTACA BstAPI 407 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 62 TTCGAA TT^CGAA BstEII 831 GGTGACC G^GT[ACGT]ACC BstPI 831 GGTGACC G^GT[ACGT]ACC DsaI 744 CCACGG C^C[GA][CT]GG EaeI 809 TGGCCG [CT]^GGCC[GA] EcoO65I 831 GGTGACC G^GT[ACGT]ACC EcoRV 202 GATATC GAT^ATC EcoT14I 249 CCTAGG C^C[AT][AT]GG FbaI 147 TGATCA T^GATCA Fnu4HI 811 GCCGC GC^[ACGT]GC HindIII 698 AAGCTT A^AGCTT ItaI 811 GCCGC GC^[ACGT]GC MaeI 250 CTAG C^TAG MaeII 644 ACGT A^CGT MspA1I 812 CCGCTG C[AC]G^C[GT]G NspV 62 TTCGAA TT^CGAA PpuMI 637 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 511 ATTAAT AT^TAAT SacI 615 GAGCTC GAGCT^C SfaNI 434 GATGC GCATC(5/9) SfuI 62 TTCGAA TT^CGAA SmlI 864 CTTGAG C^T[CT][GA]AG SspBI 647 TGTACA T^GTACA StyI 249 CCTAGG C^C[AT][AT]GG TaqI 63 TCGA T^CGA TthHB8I 63 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 12 GAGCAC G[AT]GC[AT]^C 615 GAGCTC BmyI 12 GAGCAC G[AGT]GC[ACT]^C 615 GAGCTC BpmI 9 CTGGAG CTGGAG(16/14) 55 CTGGAG BseRI 592 CTCCTC GAGGAG(10/8) 786 CTCCTC BsiHKAI 12 GAGCAC G[AT]GC[AT]^C 615 GAGCTC Bsp1286I 12 GAGCAC G[AGT]GC[ACT]^C 615 GAGCTC BspHI 398 TCATGA T^CATGA 914 TCATGA BsrDI 531 CATTGC GCAATG(2/0) 738 CATTGC DdeI 181 CTCAG C^T[ACGT]AG 613 CTGAG DpnII 148 GATC ^GATC 288 GATC DraII 409 AGGGCCT [GA]G^G[ACGT]CC[CT] 637 GGGTCCT EcoO109I 409 AGGGCCT [GA]G^G[ACGT]CC[CT] 637 GGGTCCT HinfI 60 GATTC G^A[ACGT]TC 911 GAATC MboI 148 GATC ^GATC 288 GATC MseI 103 TTAA T^TAA 512 TTAA MwoI 40 GCCTTCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 407 GCAGGGCCTGC NdeII 148 GATC ^GATC 288 GATC NlaIV 445 GGAGCC GG[ACGT]^[ACGT]CC 637 GGGTCC RcaI 398 TCATGA T^CATGA 914 TCATGA Sau3AI 148 GATC ^GATC 288 GATC SfcI 498 CTGTAG C^T[GA][CT]AG 858 CTACAG TfiI 60 GATTC G^A[AT]TC 911 GAATC Tru9I 103 TTAA T^TAA 512 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AfaI AluI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I EarI EcoRII FokI HaeIII HphI Ksp632I MaeIII MboII MnlI MslI MvaI NlaIII RsaI Sau96I ScrFI Tsp45I Tsp509I TspRI