(Circular) (Six-base) MAPSORT of: pFN21AE5979.seq Check: 3087 from: 1 to: 5644 pFN21AE5979 5644 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2534 3707 5091 5359 1632 Size: 902 1173 1384 268 1917 Fragments arranged by size: 1917 1384 1173 902 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2649 4543 5520 1203 Size: 1446 1894 977 1327 Fragments arranged by size: 1894 1446 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 2418 3387 4959 2418 Size: 969 1572 3103 Fragments arranged by size: 3103 1572 969 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 2517 4198 160 Size: 872 1485 1681 1606 Fragments arranged by size: 1681 1606 1485 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 AvrII C'CTAG_G Cuts at: 2254 2254 Size: 5644 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 4420 618 Size: 325 217 627 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 2624 2963 1391 Size: 1233 339 4072 Fragments arranged by size: 4072 1233 339 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2687 3536 4746 2687 Size: 849 1210 3585 Fragments arranged by size: 3585 1210 849 BclI T'GATC_A Cuts at: 1548 2152 1548 Size: 604 5040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 3705 4861 1790 Size: 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2649 3375 4333 Size: 416 53 83 399 24 1232 726 958 Cuts at: 4333 442 Size: 1753 Fragments arranged by size: 1753 1232 958 726 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2081 4304 1239 Size: 796 46 2223 2579 Fragments arranged by size: 2579 2223 796 46 Bpu10I CC'TnA_GC Cuts at: 2618 2618 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2890 3769 4634 4932 2890 Size: 879 865 298 3602 Fragments arranged by size: 3602 879 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2770 4307 882 Size: 617 1271 1537 2219 Fragments arranged by size: 2219 1537 1271 617 BsaAI yAC'GTr Cuts at: 493 1204 2650 3435 493 Size: 711 1446 785 2702 Fragments arranged by size: 2702 1446 785 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2588 2782 1186 Size: 1402 194 4048 Fragments arranged by size: 4048 1402 194 BseYI C'CCAG_C Cuts at: 2050 2442 4847 5343 2050 Size: 392 2405 496 2351 Fragments arranged by size: 2405 2351 496 392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 1163 Size: 573 636 4435 Fragments arranged by size: 4435 636 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2624 3705 3790 4861 2021 Size: 603 1081 85 1071 2804 Fragments arranged by size: 2804 1081 1071 603 85 BsmI GAATG_Cn' Cuts at: 2630 3155 3248 2630 Size: 525 93 5026 Fragments arranged by size: 5026 525 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2624 2963 3705 3790 4861 1391 Size: 399 231 603 339 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 339 231 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 2403 2919 3533 5263 2403 Size: 516 614 1730 2784 Fragments arranged by size: 2784 1730 614 516 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2535 2742 4139 4313 65 Size: 1183 1287 207 1397 174 1396 Fragments arranged by size: 1397 1396 1287 1183 207 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 2652 96 Size: 2556 3088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 2418 794 Size: 1624 4020 BstBI TT'CG_AA Cuts at: 2068 4448 2068 Size: 2380 3264 BstEII G'GTnAC_C Cuts at: 1184 2836 1184 Size: 1652 3992 BstYI r'GATC_y Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639 Size: 1002 367 385 17 1452 86 12 346 Cuts at: 5639 1972 Size: 1977 Fragments arranged by size: 1977 1452 1002 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2749 513 Size: 552 939 745 3408 Fragments arranged by size: 3408 939 745 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2141 487 Size: 683 56 915 3990 Fragments arranged by size: 3990 915 683 56 BtsI GCAGTG_nn' Cuts at: 2384 3160 3954 3974 2384 Size: 776 794 20 4054 Fragments arranged by size: 4054 794 776 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2814 3091 3980 Size: 54 1425 50 275 18 984 277 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 984 889 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2256 2550 3574 1353 Size: 731 172 294 1024 3423 Fragments arranged by size: 3423 1024 731 294 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2622 2622 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2081 2534 3707 4304 5091 5359 Size: 393 403 46 453 1173 597 787 268 Cuts at: 5359 1239 Size: 1524 Fragments arranged by size: 1524 1173 787 597 453 403 393 268 46 EcoO109I rG'GnC_Cy Cuts at: 2415 2643 2415 Size: 228 5416 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 EcoRV GAT'ATC Cuts at: 2209 2209 Size: 5644 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2514 2608 2514 Size: 94 5550 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 2767 2799 4285 Size: 32 135 32 568 32 595 32 1486 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1486 595 568 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 2703 748 Size: 1955 3689 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2498 4758 4942 1305 Size: 1193 2260 184 2007 Fragments arranged by size: 2260 2007 1193 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 2765 2831 3602 3961 4120 518 Size: 648 1317 282 66 771 359 159 2042 Fragments arranged by size: 2042 1317 771 648 359 282 159 66 MspA1I CmG'CkG Cuts at: 1606 2819 3060 3737 4885 5130 1606 Size: 1213 241 677 1148 245 2120 Fragments arranged by size: 2120 1213 1148 677 245 241 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 2689 1348 Size: 1341 4303 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PfoI T'CCnGG_A Cuts at: 2058 2058 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2643 2643 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SacI G_AGCT'C Cuts at: 2624 2624 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2503 2863 2992 4127 4808 4999 826 Size: 219 1458 360 129 1135 681 191 1471 Fragments arranged by size: 1471 1458 1135 681 360 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2869 3748 4649 4911 820 Size: 197 926 926 879 901 262 1553 Fragments arranged by size: 1553 926 926 901 879 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2004 2254 3083 513 Size: 552 939 250 829 3074 Fragments arranged by size: 3074 939 829 552 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2434 2702 3671 3688 3841 4026 4471 Size: 1255 854 268 969 17 153 185 445 Cuts at: 4471 325 Size: 1498 Fragments arranged by size: 1498 1255 969 854 445 268 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 2652 3890 Size: 274 80 33 51 1443 198 477 1238 Cuts at: 3890 5602 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1238 477 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 5604 5636 2430 Size: 32 3142 32 2438 Fragments arranged by size: 3142 2438 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI