################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BanII BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspHI BspMI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 203 ACATGT A^C[GA][CT]GT Aor51HI 57 AGCGCT AGC^GCT AspHI 100 GTGCTC G[AT]GC[AT]^C AvaI 618 CTCGGG C^[CT]CG[GA]G AvrII 315 CCTAGG C^CTAGG BbsI 82 GTCTTC GAAGAC(2/6) BbvCI 572 CCTCAGC CCTCAGC(-5/-2) BclI 137 TGATCA T^GATCA BfaI 316 CTAG C^TAG BlnI 315 CCTAGG C^CTAGG BlpI 292 GCTCAGC GC^T[ACGT]AGC BpmI 786 CTCCAG CTGGAG(16/14) Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC BpuAI 82 GTCTTC GAAGAC(2/6) BsaAI 97 TACGTG [CT]AC^GT[GA] BseRI 121 CTCCTC GAGGAG(10/8) BsiHKAI 100 GTGCTC G[AT]GC[AT]^C BsmAI 583 GAGAC GTCTC(1/5) BsoBI 618 CTCGGG C^[CT]CG[GA]G Bsp1407I 857 TGTACA T^GTACA BspLU11I 203 ACATGT A^CATGT BsrBI 382 CCGCTC CCGCTC(-3/-3) BsrGI 857 TGTACA T^GTACA BssSI 414 CTCGTG CACGAG(-5/-1) Cac8I 10 GCCAGC GC[ACGT]^[ACGT]GC CelII 292 GCTCAGC GC^T[ACGT]AGC DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 422 CTCTTC CTCTTC(1/4) Eco47III 57 AGCGCT AGC^GCT EcoT14I 315 CCTAGG C^C[AT][AT]GG FauI 381 CCCGC CCCGC(4/6) FbaI 137 TGATCA T^GATCA Ksp632I 422 CTCTTC CTCTTC(1/4) MaeI 316 CTAG C^TAG MaeII 98 ACGT A^CGT MspA1I 282 CAGCTG C[AC]G^C[GT]G NspI 203 ACATGT [GA]CATG^[CT] PflMI 434 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 80 GAGTC GAGTC(4/5) PvuII 282 CAGCTG CAG^CTG SfaNI 535 GATGC GCATC(5/9) SfcI 339 CTACAG C^T[GA][CT]AG SspBI 857 TGTACA T^GTACA StyI 315 CCTAGG C^C[AT][AT]GG TfiI 148 GATTC G^A[AT]TC Tsp509I 233 AATT ^AATT Van91I 434 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 64 GACGCC G[GA]^CG[CT]C 834 GACGCC AocI 774 CCTGAGG CC^T[ACGT]AGG 864 CCTGAGG AvaII 505 GGACC G^G[AT]CC 738 GGTCC BalI 368 TGGCCA TGG^CCA 428 TGGCCA BamHI 515 GGATCC G^GATCC 677 GGATCC BanI 225 GGTGCC G^G[CT][GA]CC 322 GGCACC BbvI 284 GCTGC GCAGC(8/12) 623 GCTGC BsaHI 64 GACGCC G[GA]^CG[CT]C 834 GACGCC BsgI 457 CTGCAC GTGCAG(16/14) 563 GTGCAG BstYI 515 GGATCC [GA]^GATC[CT] 677 GGATCC Bsu36I 774 CCTGAGG CC^T[ACGT]AGG 864 CCTGAGG CfoI 58 GCGC GCG^C 411 GCGC Eco81I 774 CCTGAGG CC^T[ACGT]AGG 864 CCTGAGG Fnu4HI 284 GCTGC GC^[ACGT]GC 623 GCTGC HaeII 57 AGCGCT [GA]GCGC^[CT] 410 GGCGCT HgaI 64 GACGC GACGC(5/10) 834 GACGC HhaI 58 GCGC GCG^C 411 GCGC Hin1I 64 GACGCC G[GA]^CG[CT]C 834 GACGCC HinP1I 58 GCGC G^CGC 411 GCGC HinfI 80 GAGTC G^A[ACGT]TC 148 GATTC ItaI 284 GCTGC GC^[ACGT]GC 623 GCTGC MflI 515 GGATCC [GA]^GATC[CT] 677 GGATCC MluNI 368 TGGCCA TGG^CCA 428 TGGCCA MscI 368 TGGCCA TGG^CCA 428 TGGCCA TseI 284 GCTGC G^C[AT]GC 623 GCTGC Tsp45I 220 GTCAC ^GT[GC]AC 598 GTGAC XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 788 CCAGGAAAGCTGTGG XhoII 515 GGATCC [GA]^GATC[CT] 677 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AfaI AluI AlwI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EaeI EcoRII FokI HaeIII HphI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TspRI