################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BanII BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI HapII HgaI HpaI HpaII KpnI KspI MaeI MamI MfeI MluI MroI MseI MspA1I MspI MunI MwoI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 41 CGCG CG^CG AcyI 889 GGCGCC G[GA]^CG[CT]C Alw44I 417 GTGCAC G^TGCAC ApaLI 417 GTGCAC G^TGCAC AspHI 417 GTGCAC G[AT]GC[AT]^C AviII 650 TGCGCA TGC^GCA BalI 194 TGGCCA TGG^CCA BamHI 674 GGATCC G^GATCC BanI 889 GGCGCC G^G[CT][GA]CC BbeI 889 GGCGCC GGCGC^C BsaHI 889 GGCGCC G[GA]^CG[CT]C BsaI 639 GGTCTC GGTCTC(1/5) BsaJI 440 CCTGGG C^C[ACGT][ACGT]GG BsiHKAI 417 GTGCAC G[AT]GC[AT]^C BsmAI 640 GTCTC GTCTC(1/5) BsmI 873 GAATGC GAATGC(1/-1) BspMI 712 ACCTGC ACCTGC(4/8) BstUI 41 CGCG CG^CG BstYI 674 GGATCC [GA]^GATC[CT] EarI 742 CTCTTC CTCTTC(1/4) EcoT22I 285 ATGCAT ATGCA^T FspI 650 TGCGCA TGC^GCA Hin1I 889 GGCGCC G[GA]^CG[CT]C HincII 202 GTTGAC GT[CT]^[GA]AC HindII 202 GTTGAC GT[CT]^[GA]AC HindIII 157 AAGCTT A^AGCTT KasI 889 GGCGCC G^GCGCC Ksp632I 742 CTCTTC CTCTTC(1/4) MflI 674 GGATCC [GA]^GATC[CT] MluNI 194 TGGCCA TGG^CCA MscI 194 TGGCCA TGG^CCA MvnI 41 CGCG CG^CG NarI 889 GGCGCC GG^CGCC NsiI 285 ATGCAT ATGCA^T PleI 740 GACTC GAGTC(4/5) SexAI 578 ACCAGGT A^CC[AT]GGT SfoI 889 GGCGCC GGC^GCC StuI 701 AGGCCT AGG^CCT Tsp45I 763 GTCAC ^GT[GC]AC XhoII 674 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 174 GTAC GT^AC 768 GTAC AvaII 55 GGTCC G^G[AT]CC 468 GGTCC BmyI 417 GTGCAC G[AGT]GC[ACT]^C 902 GGGCAC BpmI 64 CTGGAG CTGGAG(16/14) 408 CTGGAG BsaAI 322 CACGTA [CT]AC^GT[GA] 765 CACGTA Bsp1286I 417 GTGCAC G[AGT]GC[ACT]^C 902 GGGCAC BspHI 401 TCATGA T^CATGA 510 TCATGA BsrI 63 ACTGG ACTGG(1/-1) 678 CCAGT Cac8I 503 GCGGGC GC[ACGT]^[ACGT]GC 598 GCCTGC EaeI 194 TGGCCA [CT]^GGCC[GA] 659 TGGCCG FauI 785 CCCGC CCCGC(4/6) 503 GCGGG HaeII 754 AGCGCC [GA]GCGC^[CT] 889 GGCGCC MaeIII 389 GTTAC ^GT[ACGT]AC 763 GTCAC MboII 179 TCTTC GAAGA(8/7) 743 TCTTC RcaI 401 TCATGA T^CATGA 510 TCATGA RsaI 174 GTAC GT^AC 768 GTAC SfaNI 237 GATGC GCATC(5/9) 653 GCATC SmlI 547 CTCAAG C^T[CT][GA]AG 669 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AlwI AocI BbvI BseRI BsiYI BslI BsmFI BssKI BstF5I BstNI Bsu36I CfoI Cfr13I DdeI DpnII Eco81I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeII MboI MnlI MslI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI TseI TspRI