(Circular) (Six-base) MAPSORT of: pFN21AE5915.seq Check: 4205 from: 1 to: 5632 pFN21AE5915 5632 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 5632 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3695 5079 5347 1632 Size: 2063 1384 268 1917 Fragments arranged by size: 2063 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 2532 1166 Size: 1366 4266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 2422 3689 4845 2422 Size: 1267 1156 3209 Fragments arranged by size: 3209 1267 1156 ApoI r'AATT_y Cuts at: 1493 2941 3167 3319 5428 1493 Size: 1448 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 BamHI G'GATC_C Cuts at: 2679 2962 3329 2679 Size: 283 367 4982 Fragments arranged by size: 4982 367 283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2894 2953 4408 618 Size: 325 217 627 1107 59 1455 1842 Fragments arranged by size: 1842 1455 1107 627 325 217 59 BanII G_rGCy'C Cuts at: 1391 2951 1391 Size: 1560 4072 BbeI G_GCGC'C Cuts at: 2898 2898 Size: 5632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BfrBI ATG'CAT Cuts at: 2292 2292 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2426 2911 3693 4849 1790 Size: 636 485 782 1156 2573 Fragments arranged by size: 2573 1156 782 636 485 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2434 4292 1239 Size: 851 344 1858 2579 Fragments arranged by size: 2579 1858 851 344 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 2695 3757 4622 4920 2537 Size: 158 1062 865 298 3249 Fragments arranged by size: 3249 1062 865 298 158 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2650 4295 882 Size: 617 1151 1645 2219 Fragments arranged by size: 2219 1645 1151 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 2772 3423 493 Size: 711 1125 443 651 2702 Fragments arranged by size: 2702 1125 711 651 443 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2895 3439 3821 4456 Size: 53 83 186 936 1362 544 382 635 Cuts at: 4456 275 Size: 1451 Fragments arranged by size: 1451 1362 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2713 2816 2917 1186 Size: 883 644 103 101 3901 Fragments arranged by size: 3901 883 644 103 101 BseYI C'CCAG_C Cuts at: 2119 4835 5331 2119 Size: 2716 496 2420 Fragments arranged by size: 2716 2420 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5059 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2426 3693 3778 4849 2426 Size: 1267 85 1071 3209 Fragments arranged by size: 3209 1267 1071 85 BsmI GAATG_Cn' Cuts at: 2884 3143 3236 2884 Size: 259 93 5280 Fragments arranged by size: 5280 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2426 2911 2951 3693 3778 4849 1391 Size: 399 636 485 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 485 399 85 40 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2406 2515 3521 5251 2406 Size: 109 1006 1730 2787 Fragments arranged by size: 2787 1730 1006 109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2726 2987 844 Size: 1882 261 3489 Fragments arranged by size: 3489 1882 261 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2679 2962 3329 3714 3731 5183 5269 5281 Size: 707 283 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 707 385 367 346 283 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2492 2799 2873 2900 2492 Size: 307 74 27 5224 Fragments arranged by size: 5224 307 74 27 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2664 3079 Size: 54 1425 50 275 18 369 465 415 Cuts at: 3079 3968 5597 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 465 415 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2753 3562 1353 Size: 1400 809 3423 Fragments arranged by size: 3423 1400 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2434 3695 4292 5079 5347 1239 Size: 393 458 344 1261 597 787 268 1524 Fragments arranged by size: 1524 1261 787 597 458 393 344 268 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FspI TGC'GCA Cuts at: 2657 4138 2657 Size: 1481 4151 HaeII r_GCGC'y Cuts at: 1678 2763 2898 4779 1678 Size: 1085 135 1881 2531 Fragments arranged by size: 2531 1881 1085 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373 Size: 32 135 32 2701 32 42 32 2626 Fragments arranged by size: 2701 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2209 2976 3222 669 Size: 988 281 271 767 246 3079 Fragments arranged by size: 3079 988 767 281 271 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 4218 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KasI G'GCGC_C Cuts at: 2894 2894 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 4746 4930 1305 Size: 945 2496 184 2007 Fragments arranged by size: 2496 2007 945 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 10 Size: 54 1425 343 369 3441 Fragments arranged by size: 3441 1425 369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2532 2618 2774 3590 3949 4108 Size: 648 1008 358 86 156 816 359 159 Cuts at: 4108 518 Size: 2042 Fragments arranged by size: 2042 1008 816 648 359 358 159 156 86 MspA1I CmG'CkG Cuts at: 1606 3048 3725 4873 5118 1606 Size: 1442 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 NarI GG'CG_CC Cuts at: 2895 2895 Size: 5632 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NsiI A_TGCA'T Cuts at: 2294 2294 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SexAI A'CCwGG_T Cuts at: 2583 2583 Size: 5632 SfcI C'TryA_G Cuts at: 826 1045 2980 4115 4796 4987 826 Size: 219 1935 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2896 2896 Size: 5632 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 2674 3736 4637 4899 820 Size: 197 926 609 122 1062 901 262 1553 Fragments arranged by size: 1553 1062 926 901 609 262 197 122 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 2708 1517 Size: 1191 4441 StyI C'CwwG_G Cuts at: 513 1065 2004 3071 513 Size: 552 939 1067 3074 Fragments arranged by size: 3074 1067 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 2437 3659 3676 3829 4014 4459 Size: 1255 608 249 1222 17 153 185 445 Cuts at: 4459 325 Size: 1498 Fragments arranged by size: 1498 1255 1222 608 445 249 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 3878 5590 Size: 274 80 33 51 1443 201 1700 1712 Cuts at: 5590 96 Size: 138 Fragments arranged by size: 1712 1700 1443 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI