################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BbeI BbrPI BbvCI BbvI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII ItaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 737 CCGC CCGC(-3/-1) AlwNI 327 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG BalI 665 TGGCCA TGG^CCA BamHI 360 GGATCC G^GATCC BanI 52 GGTGCC G^G[CT][GA]CC BanII 453 GGGCTC G[GA]GC[CT]^C BbsI 684 GTCTTC GAAGAC(2/6) BcgI 412 CGAACAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 74 GTATCC GTATCC(6/5) BpuAI 684 GTCTTC GAAGAC(2/6) BsaAI 174 CACGTA [CT]AC^GT[GA] BsaJI 800 CCCAGG C^C[ACGT][ACGT]GG BsgI 692 GTGCAG GTGCAG(16/14) BsmAI 783 GTCTC GTCTC(1/5) Bsp1407I 471 TGTACA T^GTACA BsrGI 471 TGTACA T^GTACA BstAPI 313 GCAACATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DdeI 303 CTTAG C^T[ACGT]AG DraI 900 TTTAAA TTT^AAA EaeI 665 TGGCCA [CT]^GGCC[GA] EarI 79 CTCTTC CTCTTC(1/4) EcoRI 31 GAATTC G^AATTC EcoRV 208 GATATC GAT^ATC HincII 574 GTTGAC GT[CT]^[GA]AC HindII 574 GTTGAC GT[CT]^[GA]AC HinfI 154 GATTC G^A[ACGT]TC HphI 743 TCACC GGTGA(8/7) Ksp632I 79 CTCTTC CTCTTC(1/4) MaeII 175 ACGT A^CGT MluNI 665 TGGCCA TGG^CCA MscI 665 TGGCCA TGG^CCA MslI 99 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PshAI 735 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfcI 867 CTATAG C^T[GA][CT]AG SmlI 873 CTTGAG C^T[CT][GA]AG SspBI 471 TGTACA T^GTACA TfiI 154 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 31 GAATTC [GA]^AATT[CT] 267 GAATTT AfaI 177 GTAC GT^AC 472 GTAC ApoI 31 GAATTC [GA]^AATT[CT] 267 GAATTT BfaI 7 CTAG C^TAG 689 CTAG BmyI 53 GTGCCC G[AGT]GC[ACT]^C 453 GGGCTC BsmFI 757 GGGAC GGGAC(10/14) 408 GTCCC Bsp1286I 53 GTGCCC G[AGT]GC[ACT]^C 453 GGGCTC BssKI 342 CCTGG ^CC[ACGT]GG 801 CCAGG BstF5I 423 CATCC GGATG(2/0) 672 CATCC BstNI 342 CCTGG CC^[AT]GG 801 CCAGG BstYI 360 GGATCC [GA]^GATC[CT] 610 GGATCT DpnII 361 GATC ^GATC 611 GATC EcoRII 342 CCTGG ^CC[AT]GG 801 CCAGG FokI 423 CATCC GGATG(9/13) 672 CATCC HaeIII 666 GGCC GG^CC 819 GGCC HindIII 249 AAGCTT A^AGCTT 896 AAGCTT MaeI 7 CTAG C^TAG 689 CTAG MboI 361 GATC ^GATC 611 GATC MflI 360 GGATCC [GA]^GATC[CT] 610 GGATCT MvaI 342 CCTGG CC^[AT]GG 801 CCAGG MwoI 313 GCAACATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 346 GCTATGATGGC NdeII 361 GATC ^GATC 611 GATC NlaIV 52 GGTGCC GG[ACGT]^[ACGT]CC 360 GGATCC RsaI 177 GTAC GT^AC 472 GTAC Sau3AI 361 GATC ^GATC 611 GATC ScrFI 342 CCTGG CC^[ACGT]GG 801 CCAGG XhoII 360 GGATCC [GA]^GATC[CT] 610 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AflIII AluI AlwI BspLU11I BsrI MaeIII MboII MnlI MseI NlaIII NspI SfaNI Tru9I Tsp45I Tsp509I TspRI