Restriction map for
pFN21AE5901
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BbeI
BbrPI BbvI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI Hin1I HindIII HinfI HpaII
ItaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI TseI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 770 ACGTGT A^C[GA][CT]GT
AlwNI 714 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 915 CTCGGG C^[CT]CG[GA]G
BamHI 153 GGATCC G^GATCC
BanI 757 GGCACC G^G[CT][GA]CC
BanII 321 GGGCTC G[GA]GC[CT]^C
BbsI 267 GAAGAC GAAGAC(2/6)
BbvCI 675 GCTGAGG CCTCAGC(-5/-2)
BciVI 335 GTATCC GTATCC(6/5)
BmyI 321 GGGCTC G[AGT]GC[ACT]^C
BpmI 67 CTGGAG CTGGAG(16/14)
BpuAI 267 GAAGAC GAAGAC(2/6)
BsaAI 769 TACGTG [CT]AC^GT[GA]
BsoBI 915 CTCGGG C^[CT]CG[GA]G
Bsp1286I 321 GGGCTC G[AGT]GC[ACT]^C
BspHI 404 TCATGA T^CATGA
BstEII 840 GGTGACC G^GT[ACGT]ACC
BstF5I 36 CATCC GGATG(2/0)
BstPI 840 GGTGACC G^GT[ACGT]ACC
BstYI 153 GGATCC [GA]^GATC[CT]
Cac8I 429 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 295 GCGC GCG^C
DraI 852 TTTAAA TTT^AAA
DrdI 571 GACACCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 840 GGTGACC G^GT[ACGT]ACC
FokI 36 CATCC GGATG(9/13)
HaeIII 354 GGCC GG^CC
HhaI 295 GCGC GCG^C
HinP1I 295 GCGC G^CGC
HincII 247 GTTAAC GT[CT]^[GA]AC
HindII 247 GTTAAC GT[CT]^[GA]AC
HpaI 247 GTTAAC GTT^AAC
MaeII 770 ACGT A^CGT
MfeI 493 CAATTG C^AATTG
MflI 153 GGATCC [GA]^GATC[CT]
MslI 834 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 427 CAGCTG C[AC]G^C[GT]G
MunI 493 CAATTG C^AATTG
MwoI 346 GCTGACATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 836 CCATGG C^CATGG
PvuII 427 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SapI 547 GCTCTTC GCTCTTC(1/4)
SfaNI 433 GCATC GCATC(5/9)
XhoII 153 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaII 522 GGACC G^G[AT]CC
804 GGACC
BseRI 233 CTCCTC GAGGAG(10/8)
723 CTCCTC
BspMI 718 ACCTGC ACCTGC(4/8)
776 ACCTGC
BsrDI 369 CATTGC GCAATG(2/0)
11 GCAATG
BssKI 443 CCTGG ^CC[ACGT]GG
801 CCAGG
BstNI 443 CCTGG CC^[AT]GG
801 CCAGG
Cfr13I 522 GGACC G^G[ACGT]CC
804 GGACC
DsaI 412 CCACGG C^C[GA][CT]GG
836 CCATGG
EcoRII 443 CCTGG ^CC[AT]GG
801 CCAGG
MaeIII 788 GTGAC ^GT[ACGT]AC
841 GTGAC
MvaI 443 CCTGG CC^[AT]GG
801 CCAGG
PflMI 423 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCATAGGCTGG
Sau96I 522 GGACC G^G[ACGT]CC
804 GGACC
ScrFI 443 CCTGG CC^[ACGT]GG
801 CCAGG
SmlI 553 CTCAAG C^T[CT][GA]AG
665 CTCAAG
Tsp45I 788 GTGAC ^GT[GC]AC
841 GTGAC
Van91I 423 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCATAGGCTGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
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AfaI AluI AlwI BfaI BsaJI
BsiYI BslI BsrI DdeI DpnII
EarI EcoT14I HphI Ksp632I MaeI
MboI MboII MnlI MseI NdeII
NlaIII NlaIV RsaI Sau3AI StyI
Tru9I Tsp509I TspRI