(Circular) (Six-base) MAPSORT of: pFN21AE5901.seq Check: 591 from: 1 to: 5632 pFN21AE5901 5632 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5632 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2701 3695 5079 5347 1632 Size: 1069 994 1384 268 1917 Fragments arranged by size: 1917 1384 1069 994 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 2775 4531 5508 1203 Size: 1572 1756 977 1327 Fragments arranged by size: 1756 1572 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 2843 1166 Size: 1677 3955 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AlwNI CAG_nnn'CTG Cuts at: 2724 3375 4947 2724 Size: 651 1572 3409 Fragments arranged by size: 3409 1572 651 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2941 3167 3319 5428 1493 Size: 1448 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2920 2957 1870 Size: 73 977 37 4545 Fragments arranged by size: 4545 977 73 37 BamHI G'GATC_C Cuts at: 2158 2962 3329 2158 Size: 804 367 4461 Fragments arranged by size: 4461 804 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2762 2953 4408 618 Size: 325 217 627 975 191 1455 1842 Fragments arranged by size: 1842 1455 975 627 325 217 191 BanII G_rGCy'C Cuts at: 1391 2330 2951 1391 Size: 939 621 4072 Fragments arranged by size: 4072 939 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2279 928 Size: 971 380 4281 Fragments arranged by size: 4281 971 380 BbvCI CC'TCA_GC Cuts at: 2681 2681 Size: 5632 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2351 3524 4734 2351 Size: 1173 1210 3249 Fragments arranged by size: 3249 1210 1173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 3693 4849 1790 Size: 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2171 2333 3363 Size: 416 53 83 399 24 754 162 1030 Cuts at: 3363 4321 442 Size: 958 1753 Fragments arranged by size: 1753 1030 958 754 416 399 162 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 4292 1239 Size: 854 2199 2579 Fragments arranged by size: 2579 2199 854 Bpu10I CC'TnA_GC Cuts at: 2681 2681 Size: 5632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 2655 3757 4622 4920 2543 Size: 112 1102 865 298 3255 Fragments arranged by size: 3255 1102 865 298 112 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2776 3423 493 Size: 711 1572 647 2702 Fragments arranged by size: 2702 1572 711 647 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2229 2719 1186 Size: 1043 490 4099 Fragments arranged by size: 4099 1043 490 BseYI C'CCAG_C Cuts at: 4835 5331 4835 Size: 496 5136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5059 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3693 3778 4849 3693 Size: 85 1071 4476 Fragments arranged by size: 4476 1071 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2951 3693 3778 4849 1391 Size: 399 540 621 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 540 399 85 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2409 3521 5251 2409 Size: 1112 1730 2790 Fragments arranged by size: 2790 1730 1112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 2790 2987 844 Size: 1888 58 197 3489 Fragments arranged by size: 3489 1888 197 58 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2023 2373 4127 4301 65 Size: 1183 775 350 1754 174 1396 Fragments arranged by size: 1754 1396 1183 775 350 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 2845 1184 Size: 1661 3971 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2158 2962 3329 3714 3731 5183 5269 5281 Size: 186 804 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 804 385 367 346 186 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2417 2841 513 Size: 552 939 413 424 3304 Fragments arranged by size: 3304 939 552 424 413 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2859 2936 2859 Size: 77 5555 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2582 4639 809 Size: 507 1266 2057 1802 Fragments arranged by size: 2057 1802 1266 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597 Size: 54 1425 50 275 18 1249 889 1629 Cuts at: 5597 8 Size: 43 Fragments arranged by size: 1629 1425 1249 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2559 2837 3562 1353 Size: 819 387 278 725 3423 Fragments arranged by size: 3423 819 725 387 278 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2701 3695 4292 5079 5347 1239 Size: 393 461 608 994 597 787 268 1524 Fragments arranged by size: 1524 994 787 608 597 461 393 268 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373 Size: 32 135 32 2701 32 42 32 2626 Fragments arranged by size: 2701 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 2976 3222 669 Size: 988 281 316 722 246 3079 Fragments arranged by size: 3079 988 722 316 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 2254 3222 2254 Size: 968 4664 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 2498 3231 2498 Size: 733 4899 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3810 Fragments arranged by size: 3810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 3590 3949 4108 518 Size: 648 1677 747 359 159 2042 Fragments arranged by size: 2042 1677 747 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2434 3048 3725 4873 5118 1606 Size: 828 614 677 1148 245 2120 Fragments arranged by size: 2120 1148 828 677 614 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2841 513 Size: 552 939 837 3304 Fragments arranged by size: 3304 939 837 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2434 2524 1108 Size: 1326 90 4216 Fragments arranged by size: 4216 1326 90 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 PvuII CAG'CTG Cuts at: 2434 2434 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SapI GCTCTTCn'nnn_ Cuts at: 2559 2559 Size: 5632 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2980 4115 4796 4987 826 Size: 219 1935 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 2670 3736 4637 4899 820 Size: 197 926 615 112 1066 901 262 1553 Fragments arranged by size: 1553 1066 926 901 615 262 197 112 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 2812 2841 3071 513 Size: 552 939 357 451 29 230 3074 Fragments arranged by size: 3074 939 552 451 357 230 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3878 5590 Size: 274 80 33 51 1443 204 1697 1712 Cuts at: 5590 96 Size: 138 Fragments arranged by size: 1712 1697 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2845 2877 5592 5624 2845 Size: 32 2715 32 2853 Fragments arranged by size: 2853 2715 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI