Restriction map for
pFN21AE3711
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HgaI HincII HindII
HpaI KspI MaeII MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I Tth111I XbaI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 958 GGTACC G^GTACC
AcsI 738 GAATTT [GA]^AATT[CT]
AcyI 839 GGCGCC G[GA]^CG[CT]C
AflIII 587 ACATGT A^C[GA][CT]GT
AlwNI 158 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 946 GGGCCC GGGCC^C
ApoI 738 GAATTT [GA]^AATT[CT]
Asp718I 958 GGTACC G^GTACC
BbeI 839 GGCGCC GGCGC^C
BcgI 438 CGAGCAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 842 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 640 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 839 GGCGCC G[GA]^CG[CT]C
BseRI 999 CTCCTC GAGGAG(10/8)
BsmI 1068 GCATTC GAATGC(1/-1)
BspLU11I 587 ACATGT A^CATGT
BsrFI 836 ACCGGC [GA]^CCGG[CT]
BstXI 789 CCAGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 840 GCGC GCG^C
Cfr10I 836 ACCGGC [GA]^CCGG[CT]
DsaI 64 CCATGG C^C[GA][CT]GG
EarI 465 GAAGAG CTCTTC(1/4)
EcoT22I 1066 ATGCAT ATGCA^T
HaeII 839 GGCGCC [GA]GCGC^[CT]
HhaI 840 GCGC GCG^C
Hin1I 839 GGCGCC G[GA]^CG[CT]C
HinP1I 840 GCGC G^CGC
HindIII 108 AAGCTT A^AGCTT
KasI 839 GGCGCC G^GCGCC
KpnI 958 GGTACC GGTAC^C
Ksp632I 465 GAAGAG CTCTTC(1/4)
MaeIII 833 GTAAC ^GT[ACGT]AC
MamI 640 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 839 GGCGCC GG^CGCC
NcoI 64 CCATGG C^CATGG
NsiI 1066 ATGCAT ATGCA^T
NspI 587 ACATGT [GA]CATG^[CT]
PleI 299 GAGTC GAGTC(4/5)
PshAI 920 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 839 GGCGCC GGC^GCC
XcmI 114 CCAAGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 91 GATCC GGATC(4/5)
470 GGATC
AocI 232 CCTCAGG CC^T[ACGT]AGG
1015 CCTGAGG
AvaI 436 CTCGAG C^[CT]CG[GA]G
802 CTCGAG
AvaII 816 GGTCC G^G[AT]CC
919 GGACC
BfaI 250 CTAG C^TAG
385 CTAG
BlpI 105 GCTAAGC GC^T[ACGT]AGC
573 GCTTAGC
Bpu1102I 105 GCTAAGC GC^T[ACGT]AGC
573 GCTTAGC
BsaI 271 GAGACC GGTCTC(1/5)
902 GGTCTC
BsgI 342 CTGCAC GTGCAG(16/14)
989 CTGCAC
BsoBI 436 CTCGAG C^[CT]CG[GA]G
802 CTCGAG
Bsu36I 232 CCTCAGG CC^T[ACGT]AGG
1015 CCTGAGG
Cac8I 681 GCAGGC GC[ACGT]^[ACGT]GC
851 GCAAGC
CelII 105 GCTAAGC GC^T[ACGT]AGC
573 GCTTAGC
Eco81I 232 CCTCAGG CC^T[ACGT]AGG
1015 CCTGAGG
FauI 192 CCCGC CCCGC(4/6)
4 GCGGG
HphI 55 GGTGA GGTGA(8/7)
473 TCACC
MaeI 250 CTAG C^TAG
385 CTAG
MboII 465 GAAGA GAAGA(8/7)
648 TCTTC
PaeR7I 436 CTCGAG C^TCGAG
802 CTCGAG
PflMI 43 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCAGGCTATGG
SmlI 436 CTCGAG C^T[CT][GA]AG
802 CTCGAG
TfiI 137 GAATC G^A[AT]TC
177 GATTC
Tsp509I 443 AATT ^AATT
739 AATT
Van91I 43 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCAGGCTATGG
XhoI 436 CTCGAG C^TCGAG
802 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI Asp700I BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI TspRI TthHB8I
XmnI