(Circular) (Six-base) MAPSORT of: pFN21AE3711.seq Check: 8277 from: 1 to: 5782 pFN21AE3711 5782 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 2963 3103 2963 Size: 140 5642 AclI AA'CG_TT Cuts at: 1563 3081 3909 4282 1563 Size: 1518 828 373 3063 Fragments arranged by size: 3063 1518 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2483 2639 3845 5229 5497 1632 Size: 851 156 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 851 268 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2592 4681 5658 1203 Size: 1389 2089 977 1327 Fragments arranged by size: 2089 1389 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2732 2764 2732 Size: 32 5750 AlwNI CAG_nnn'CTG Cuts at: 2168 3525 5097 2168 Size: 1357 1572 2853 Fragments arranged by size: 2853 1572 1357 ApaI G_GGCC'C Cuts at: 2955 2955 Size: 5782 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2743 3091 3317 3469 5578 1493 Size: 1250 348 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 348 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2441 2807 3107 1870 Size: 73 498 366 300 4545 Fragments arranged by size: 4545 498 366 300 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2844 2963 3103 4558 Size: 325 217 627 576 481 119 140 1455 Cuts at: 4558 618 Size: 1842 Fragments arranged by size: 1842 1455 627 576 481 325 217 140 119 BanII G_rGCy'C Cuts at: 1391 2511 2808 2955 2984 3065 3101 1391 Size: 1120 297 147 29 81 36 4072 Fragments arranged by size: 4072 1120 297 147 81 36 29 BbeI G_GCGC'C Cuts at: 2848 2848 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2432 2466 3973 4007 1903 Size: 34 495 34 1507 34 3678 Fragments arranged by size: 3678 1507 495 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BfrBI ATG'CAT Cuts at: 3073 3073 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2853 4393 136 Size: 107 122 71 631 605 1181 1540 1525 Fragments arranged by size: 1540 1525 1181 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2111 2579 3199 2111 Size: 468 620 4694 Fragments arranged by size: 4694 620 468 Bme1580I G_kGCm'C Cuts at: 1790 2262 2762 2955 3843 4999 1790 Size: 472 500 193 888 1156 2573 Fragments arranged by size: 2573 1156 888 500 472 193 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3035 3513 4471 Size: 416 53 83 399 24 1618 478 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1618 958 478 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 2151 2396 2759 4442 1239 Size: 792 120 245 363 1683 2579 Fragments arranged by size: 2579 1683 792 363 245 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 2913 4445 882 Size: 617 771 643 1532 2219 Fragments arranged by size: 2219 1532 771 643 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 2649 5776 2649 Size: 3127 2655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2845 3589 3971 4606 Size: 53 83 186 936 1312 744 382 635 Cuts at: 4606 275 Size: 1451 Fragments arranged by size: 1451 1312 936 744 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2295 2325 2732 2762 1178 Size: 30 165 30 892 30 407 30 4198 Fragments arranged by size: 4198 892 407 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2995 1186 Size: 1809 3973 BseYI C'CCAG_C Cuts at: 2088 2793 2903 4985 5481 2088 Size: 705 110 2082 496 2389 Fragments arranged by size: 2389 2082 705 496 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2332 2979 1163 Size: 573 596 647 3966 Fragments arranged by size: 3966 647 596 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 3928 4999 3843 Size: 85 1071 4626 Fragments arranged by size: 4626 1071 85 BsmI GAATG_Cn' Cuts at: 3073 3293 3386 3073 Size: 220 93 5469 Fragments arranged by size: 5469 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2511 2762 2808 2955 2984 3065 Size: 399 472 249 251 46 147 29 81 Cuts at: 3065 3101 3843 3928 4999 1391 Size: 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 251 249 147 85 81 46 36 29 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3137 844 Size: 2293 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2841 4426 4613 1700 Size: 110 1031 1585 187 2869 Fragments arranged by size: 2869 1585 1031 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 5782 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2238 3021 2238 Size: 783 4999 BtgI C'CryG_G Cuts at: 513 1065 2004 2069 513 Size: 552 939 65 4226 Fragments arranged by size: 4226 939 552 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2773 487 Size: 683 56 1547 3496 Fragments arranged by size: 3496 1547 683 56 BtsI GCAGTG_nn' Cuts at: 2762 2771 2991 3298 4092 4112 2762 Size: 9 220 307 794 20 4432 Fragments arranged by size: 4432 794 307 220 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747 Size: 54 1425 50 275 18 1399 889 1629 Cuts at: 5747 8 Size: 43 Fragments arranged by size: 1629 1425 1399 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2465 3712 1353 Size: 1112 1247 3423 Fragments arranged by size: 3423 1247 1112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 4369 4743 4889 387 Size: 1064 138 504 2276 374 146 1280 Fragments arranged by size: 2276 1280 1064 504 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2151 2396 2483 2639 2759 3845 Size: 393 399 120 245 87 156 120 1086 Cuts at: 3845 4442 5229 5497 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1086 787 597 399 393 268 245 156 120 120 87 EcoNI CCTnn'n_nnAGG Cuts at: 2130 2684 2916 2130 Size: 554 232 4996 Fragments arranged by size: 4996 554 232 EcoO109I rG'GnC_Cy Cuts at: 2123 2331 2951 2952 2123 Size: 208 620 1 4953 Fragments arranged by size: 4953 620 208 1 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2848 4929 1678 Size: 1170 2081 2531 Fragments arranged by size: 2531 2081 1170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 2113 748 Size: 1365 4417 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 2844 2844 Size: 5782 KpnI G_GTAC'C Cuts at: 2967 3107 2967 Size: 140 5642 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 4896 5080 1305 Size: 919 2672 184 2007 Fragments arranged by size: 2672 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4099 4258 518 Size: 648 2574 359 159 2042 Fragments arranged by size: 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3198 3875 5023 5268 1606 Size: 1592 677 1148 245 2120 Fragments arranged by size: 2120 1592 1148 677 245 NarI GG'CG_CC Cuts at: 2845 2845 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2004 2069 513 Size: 552 939 65 4226 Fragments arranged by size: 4226 939 552 65 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NsiI A_TGCA'T Cuts at: 3075 3075 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2596 3140 4685 1132 Size: 97 1367 544 1545 2229 Fragments arranged by size: 2229 1545 1367 544 97 PasI CC'CwG_GG Cuts at: 1348 2482 2948 1348 Size: 1134 466 4182 Fragments arranged by size: 4182 1134 466 PciI A'CATG_T Cuts at: 2592 4681 2592 Size: 2089 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 2054 2975 1108 Size: 946 921 3915 Fragments arranged by size: 3915 946 921 PfoI T'CCnGG_A Cuts at: 2128 2128 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2732 2764 3851 3883 5364 5396 2732 Size: 32 1087 32 1481 32 3118 Fragments arranged by size: 3118 1481 1087 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2929 1613 Size: 1316 4466 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PspOMI G'GGCC_C Cuts at: 2951 2951 Size: 5782 PspXI vC'TCGA_Gb Cuts at: 2441 2441 Size: 5782 PstI C_TGCA'G Cuts at: 830 2433 2577 2619 2757 2778 3134 830 Size: 1603 144 42 138 21 356 3478 Fragments arranged by size: 3478 1603 356 144 138 42 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2429 2573 2615 2753 2774 3130 4265 Size: 219 1384 144 42 138 21 356 1135 Cuts at: 4265 4946 5137 826 Size: 681 191 1471 Fragments arranged by size: 1471 1384 1135 681 356 219 191 144 138 42 21 SfoI GGC'GCC Cuts at: 2846 2846 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2441 2807 3886 4787 5049 820 Size: 197 926 498 366 1079 901 262 1553 Fragments arranged by size: 1553 1079 926 901 498 366 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2004 2069 2119 2263 3221 513 Size: 552 939 65 50 144 958 3074 Fragments arranged by size: 3074 958 939 552 144 65 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2614 3809 3826 3979 4164 4609 325 Size: 1255 1034 1195 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1195 1034 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3000 4028 5740 Size: 274 80 33 51 1443 1023 1028 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1443 1028 1023 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2295 2327 5742 5774 2295 Size: 32 3415 32 2303 Fragments arranged by size: 3415 2303 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2126 2126 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 2441 2807 1943 Size: 498 366 4918 Fragments arranged by size: 4918 498 366 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 2537 2652 2747 3033 3911 2537 Size: 115 95 286 878 4408 Fragments arranged by size: 4408 878 286 115 95 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI