Restriction map for
pFN21AE3711
(Linear) (Six-base) MAPSORT of: KIEE3711.seq Check: 2992 from: 1 to: 1077
KIEE3711.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
Acc65I G'GTAC_C
Cuts at: 0 958 1077
Size: 958 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 634 1077
Size: 478 156 443
Fragments arranged by size:
478 443 156
AflIII A'CryG_T
Cuts at: 0 587 1077
Size: 587 490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 1077
Size: 727 32 318
Fragments arranged by size:
727 318 32
AlwNI CAG_nnn'CTG
Cuts at: 0 163 1077
Size: 163 914
ApaI G_GGCC'C
Cuts at: 0 950 1077
Size: 950 127
ApoI r'AATT_y
Cuts at: 0 738 1077
Size: 738 339
AvaI C'yCGr_G
Cuts at: 0 436 802 1077
Size: 436 366 275
Fragments arranged by size:
436 366 275
BanI G'GyrC_C
Cuts at: 0 358 839 958 1077
Size: 358 481 119 119
Fragments arranged by size:
481 358 119 119
BanII G_rGCy'C
Cuts at: 0 506 803 950 979 1060 1077
Size: 506 297 147 29 81 17
Fragments arranged by size:
506 297 147 81 29 17
BbeI G_GCGC'C
Cuts at: 0 843 1077
Size: 843 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 461 1077
Size: 427 34 616
Fragments arranged by size:
616 427 34
BfrBI ATG'CAT
Cuts at: 0 1068 1077
Size: 1068 9
BglI GCCn_nnn'nGGC
Cuts at: 0 848 1077
Size: 848 229
BlpI GC'TnA_GC
Cuts at: 0 106 574 1077
Size: 106 468 503
Fragments arranged by size:
503 468 106
Bme1580I G_kGCm'C
Cuts at: 0 257 757 950 1077
Size: 257 500 193 127
Fragments arranged by size:
500 257 193 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 1030 1077
Size: 1030 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 333 1077
Size: 333 744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 146 391 754 1077
Size: 26 120 245 363 323
Fragments arranged by size:
363 323 245 120 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 908 1077
Size: 265 643 169
Fragments arranged by size:
643 265 169
BsaBI GATnn'nnATC
Cuts at: 0 644 1077
Size: 644 433
BsaHI Gr'CG_yC
Cuts at: 0 840 1077
Size: 840 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 290 320 727 757 1077
Size: 290 30 407 30 320
Fragments arranged by size:
407 320 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 990 1077
Size: 990 87
BseYI C'CCAG_C
Cuts at: 0 83 788 898 1077
Size: 83 705 110 179
Fragments arranged by size:
705 179 110 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 974 1077
Size: 327 647 103
Fragments arranged by size:
647 327 103
BsmI GAATG_Cn'
Cuts at: 0 1068 1077
Size: 1068 9
Bsp1286I G_dGCh'C
Cuts at: 0 257 506 757 803 950 979 1060 1077
Size: 257 249 251 46 147 29 81 17
Fragments arranged by size:
257 251 249 147 81 46 29 17
BsrFI r'CCGG_y
Cuts at: 0 836 1077
Size: 836 241
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 1077
Size: 796 281
Bsu36I CC'TnA_GG
Cuts at: 0 233 1016 1077
Size: 233 783 61
Fragments arranged by size:
783 233 61
BtgI C'CryG_G
Cuts at: 0 64 1077
Size: 64 1013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 768 1077
Size: 768 309
BtsI GCAGTG_nn'
Cuts at: 0 757 766 986 1077
Size: 757 9 220 91
Fragments arranged by size:
757 220 91 9
EarI CTCTTCn'nnn_
Cuts at: 0 460 1077
Size: 460 617
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 1077
Size: 88 989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 146 391 478 634 754 1077
Size: 26 120 245 87 156 120 323
Fragments arranged by size:
323 245 156 120 120 87 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 125 679 911 1077
Size: 125 554 232 166
Fragments arranged by size:
554 232 166 125
EcoO109I rG'GnC_Cy
Cuts at: 0 118 326 946 947 1077
Size: 118 208 620 1 130
Fragments arranged by size:
620 208 130 118 1
HaeII r_GCGC'y
Cuts at: 0 843 1077
Size: 843 234
HindIII A'AGCT_T
Cuts at: 0 108 1077
Size: 108 969
KasI G'GCGC_C
Cuts at: 0 839 1077
Size: 839 238
KpnI G_GTAC'C
Cuts at: 0 962 1077
Size: 962 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1077
Size: 219 858
NarI GG'CG_CC
Cuts at: 0 840 1077
Size: 840 237
NcoI C'CATG_G
Cuts at: 0 64 1077
Size: 64 1013
NsiI A_TGCA'T
Cuts at: 0 1070 1077
Size: 1070 7
NspI r_CATG'y
Cuts at: 0 591 1077
Size: 591 486
PasI CC'CwG_GG
Cuts at: 0 477 943 1077
Size: 477 466 134
Fragments arranged by size:
477 466 134
PciI A'CATG_T
Cuts at: 0 587 1077
Size: 587 490
PflMI CCAn_nnn'nTGG
Cuts at: 0 49 970 1077
Size: 49 921 107
Fragments arranged by size:
921 107 49
PfoI T'CCnGG_A
Cuts at: 0 123 1077
Size: 123 954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 1077
Size: 727 32 318
Fragments arranged by size:
727 318 32
PshAI GACnn'nnGTC
Cuts at: 0 924 1077
Size: 924 153
PspOMI G'GGCC_C
Cuts at: 0 946 1077
Size: 946 131
PspXI vC'TCGA_Gb
Cuts at: 0 436 1077
Size: 436 641
PstI C_TGCA'G
Cuts at: 0 428 572 614 752 773 1077
Size: 428 144 42 138 21 304
Fragments arranged by size:
428 304 144 138 42 21
SfcI C'TryA_G
Cuts at: 0 424 568 610 748 769 1077
Size: 424 144 42 138 21 308
Fragments arranged by size:
424 308 144 138 42 21
SfoI GGC'GCC
Cuts at: 0 841 1077
Size: 841 236
SmlI C'TyrA_G
Cuts at: 0 436 802 1077
Size: 436 366 275
Fragments arranged by size:
436 366 275
StyI C'CwwG_G
Cuts at: 0 64 114 258 1077
Size: 64 50 144 819
Fragments arranged by size:
819 144 64 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 1077
Size: 609 468
TatI w'GTAC_w
Cuts at: 0 995 1077
Size: 995 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 290 322 1077
Size: 290 32 755
Fragments arranged by size:
755 290 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 121 1077
Size: 121 956
XhoI C'TCGA_G
Cuts at: 0 436 802 1077
Size: 436 366 275
Fragments arranged by size:
436 366 275
XmnI GAAnn'nnTTC
Cuts at: 0 532 647 742 1028 1077
Size: 532 115 95 286 49
Fragments arranged by size:
532 286 115 95 49
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BsrFI BstXI Bsu36I BtgI
BtgZI BtsI EarI EciI Eco57MI EcoNI EcoO109I HaeII
HindIII KasI KpnI MmeI NarI NcoI NsiI NspI
PasI PciI PflMI PfoI PpiI PshAI PspOMI PspXI
PstI SfcI SfoI SmlI StyI TaqII TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BglII BmgBI BmtI
Bpu10I BpuEI BsaAI BsaWI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HpaI MfeI MluI
MscI MslI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI PacI PmeI PmlI PpuMI PsiI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI
ZraI