Restriction map for
pFN21AE5194
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaI HpaII HphI
KasI KspI MaeI MaeIII MfeI
MluI MroI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AciI 314 GCGG CCGC(-3/-1)
AlwI 396 GATCC GGATC(4/5)
AocI 343 CCTGAGG CC^T[ACGT]AGG
Asp700I 258 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 106 TGGCCA TGG^CCA
BbsI 138 GAAGAC GAAGAC(2/6)
BbvCI 41 CCTCAGC CCTCAGC(-5/-2)
BbvI 433 GCAGC GCAGC(8/12)
BclI 401 TGATCA T^GATCA
BlpI 65 GCTCAGC GC^T[ACGT]AGC
BpmI 304 CTCCAG CTGGAG(16/14)
Bpu1102I 65 GCTCAGC GC^T[ACGT]AGC
BpuAI 138 GAAGAC GAAGAC(2/6)
BsaAI 74 TACGTA [CT]AC^GT[GA]
BsaI 350 GAGACC GGTCTC(1/5)
BseRI 427 GAGGAG GAGGAG(10/8)
BsmBI 337 CGTCTC CGTCTC(1/5)
BsmFI 241 GTCCC GGGAC(10/14)
BstAPI 433 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 135 TTCGAA TT^CGAA
BstYI 395 AGATCC [GA]^GATC[CT]
Bsu36I 343 CCTGAGG CC^T[ACGT]AGG
CelII 65 GCTCAGC GC^T[ACGT]AGC
DsaI 452 CCATGG C^C[GA][CT]GG
EarI 391 GAAGAG CTCTTC(1/4)
Eco81I 343 CCTGAGG CC^T[ACGT]AGG
EcoNI 354 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 211 GATATC GAT^ATC
FauI 314 GCGGG CCCGC(4/6)
FbaI 401 TGATCA T^GATCA
Fnu4HI 433 GCAGC GC^[ACGT]GC
HincII 222 GTCAAC GT[CT]^[GA]AC
HindII 222 GTCAAC GT[CT]^[GA]AC
ItaI 433 GCAGC GC^[ACGT]GC
Ksp632I 391 GAAGAG CTCTTC(1/4)
MaeII 75 ACGT A^CGT
MflI 395 AGATCC [GA]^GATC[CT]
MluNI 106 TGGCCA TGG^CCA
MscI 106 TGGCCA TGG^CCA
NcoI 452 CCATGG C^CATGG
NspV 135 TTCGAA TT^CGAA
PpuMI 329 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 331 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 135 TTCGAA TT^CGAA
SnaBI 74 TACGTA TAC^GTA
TseI 433 GCAGC G^C[AT]GC
XcmI 161 CCAAGGTCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 395 AGATCC [GA]^GATC[CT]
XmnI 258 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 290 GGTACC G^GTACC
321 GGTACC
AluI 269 AGCT AG^CT
435 AGCT
Asp718I 290 GGTACC G^GTACC
321 GGTACC
AspHI 63 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
AvaII 165 GGTCC G^G[AT]CC
330 GGACC
BanI 290 GGTACC G^G[CT][GA]CC
321 GGTACC
BmyI 63 GTGCTC G[AGT]GC[ACT]^C
94 GTGCTC
BsaBI 273 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
396 GATCCTGATC
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C
94 GTGCTC
BsrI 142 ACTGG ACTGG(1/-1)
193 ACTGG
BssKI 282 CCAGG ^CC[ACGT]GG
306 CCAGG
BstNI 282 CCAGG CC^[AT]GG
306 CCAGG
BstXI 168 CCATCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
267 CCAGCTGATTGG
Cac8I 104 GCTGGC GC[ACGT]^[ACGT]GC
146 GCAAGC
DraII 37 GGGGCCT [GA]G^G[ACGT]CC[CT]
329 AGGACCT
EaeI 106 TGGCCA [CT]^GGCC[GA]
153 CGGCCA
EcoO109I 37 GGGGCCT [GA]G^G[ACGT]CC[CT]
329 AGGACCT
EcoRII 282 CCAGG ^CC[AT]GG
306 CCAGG
KpnI 290 GGTACC GGTAC^C
321 GGTACC
MamI 273 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
396 GATCCTGATC
MvaI 282 CCAGG CC^[AT]GG
306 CCAGG
PvuII 268 CAGCTG CAG^CTG
434 CAGCTG
ScrFI 282 CCAGG CC^[ACGT]GG
306 CCAGG
SfaNI 31 GATGC GCATC(5/9)
278 GCATC
TaqI 20 TCGA T^CGA
136 TCGA
TspRI 61 CAGTG CA[GC]TG[ACGT][ACGT]^
299 CACTG
TthHB8I 20 TCGA T^CGA
136 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI BsaJI BsiYI BslI BsmAI
BstF5I Cfr13I DdeI DpnII EcoT14I
FokI HaeIII MboI MboII MnlI
MspA1I MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I StyI