################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccII AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BlnI BsgI BsiEI BsiWI BspDI BspHI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI HaeII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 319 GTAGAC GT^[AC][GT]AC AccIII 2582 TCCGGA T^CCGGA AcyI 4184 GACGCC G[GA]^CG[CT]C Alw44I 1934 GTGCAC G^TGCAC ApaLI 1934 GTGCAC G^TGCAC Asp700I 742 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 3410 CACGTG CAC^GTG BbvCI 24 GCTGAGG CCTCAGC(-5/-2) BclI 2345 TGATCA T^GATCA BglI 1352 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 4184 GACGCC G[GA]^CG[CT]C BsaI 4708 GAGACC GGTCTC(1/5) BsaWI 2582 TCCGGA [AT]^CCGG[AT] BseAI 2582 TCCGGA T^CCGGA BsmBI 5030 CGTCTC CGTCTC(1/5) BspEI 2582 TCCGGA T^CCGGA BspMI 2133 GCAGGT ACCTGC(4/8) BstAPI 3853 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3924 TTCGAA TT^CGAA EcoNI 5277 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1673 GAATTC G^AATTC EcoRV 1315 GATATC GAT^ATC EcoT22I 2458 ATGCAT ATGCA^T FbaI 2345 TGATCA T^GATCA Hin1I 4184 GACGCC G[GA]^CG[CT]C HincII 3153 GTCAAC GT[CT]^[GA]AC HindII 3153 GTCAAC GT[CT]^[GA]AC HindIII 3781 AAGCTT A^AGCTT MroI 2582 TCCGGA T^CCGGA NcoI 1747 CCATGG C^CATGG NdeI 3871 CATATG CA^TATG NsiI 2458 ATGCAT ATGCA^T NspV 3924 TTCGAA TT^CGAA PaeR7I 5033 CTCGAG C^TCGAG PmaCI 3410 CACGTG CAC^GTG PmlI 3410 CACGTG CAC^GTG PshAI 3037 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2275 CTGCAG CTGCA^G ScaI 1922 AGTACT AGT^ACT SfuI 3924 TTCGAA TT^CGAA SmaI 483 CCCGGG CCC^GGG SphI 5442 GCATGC GCATG^C SspI 1816 AATATT AAT^ATT XcmI 1058 CCAAACCAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 5033 CTCGAG C^TCGAG XmaCI 483 CCCGGG C^CCGGG XmaI 483 CCCGGG C^CCGGG XmnI 742 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1721 ACATGT A^C[GA][CT]GT 3374 ACATGT AspI 3542 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4206 GACGCTGTC BciVI 2528 GGATAC GTATCC(6/5) 116 GTATCC BlpI 3223 GCTGAGC GC^T[ACGT]AGC 3467 GCTCAGC Bpu1102I 3223 GCTGAGC GC^T[ACGT]AGC 3467 GCTCAGC BsaBI 2080 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5221 GATGGTGATC Bsp1407I 4912 TGTACA T^GTACA 5253 TGTACA BspLU11I 1721 ACATGT A^CATGT 3374 ACATGT BsrBI 140 GAGCGG CCGCTC(-3/-3) 3430 GAGCGG BsrGI 4912 TGTACA T^GTACA 5253 TGTACA CelII 3223 GCTGAGC GC^T[ACGT]AGC 3467 GCTCAGC DraIII 2925 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4343 CACTCCGTG DrdI 4591 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4808 GACCTGAGAGTC MamI 2080 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5221 GATGGTGATC PflFI 3542 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4206 GACGCTGTC PflMI 688 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 882 CCAGGAGGTGG SapI 871 GAAGAGC GCTCTTC(1/4) 3946 GAAGAGC SnaBI 3077 TACGTA TAC^GTA 4715 TACGTA SspBI 4912 TGTACA T^GTACA 5253 TGTACA Tth111I 3542 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4206 GACGCTGTC Van91I 688 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 882 CCAGGAGGTGG XbaI 1192 TCTAGA T^CTAGA 4761 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII