(Circular) (Six-base) MAPSORT of: pFN21AE3488.seq Check: 1897 from: 1 to: 10183 pFN21AE3488 10183 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7993 278 Size: 53 83 186 936 6457 2468 Fragments arranged by size: 6457 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2325 7526 1937 Size: 388 5201 4594 Fragments arranged by size: 5201 4594 388 Acc65I G'GTAC_C Cuts at: 7504 7504 Size: 10183 AclI AA'CG_TT Cuts at: 1563 8310 8683 1563 Size: 6747 373 3063 Fragments arranged by size: 6747 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3686 4775 5358 7290 7326 7427 7437 8246 Size: 2054 1089 583 1932 36 101 10 809 Cuts at: 8246 9630 9898 1632 Size: 1384 268 1917 Fragments arranged by size: 2054 1932 1917 1384 1089 809 583 268 101 36 10 AflII C'TTAA_G Cuts at: 820 1017 4652 5528 5762 5789 820 Size: 197 3635 876 234 27 5214 Fragments arranged by size: 5214 3635 876 234 197 27 AflIII A'CryG_T Cuts at: 1203 3726 5379 9082 10059 1203 Size: 2523 1653 3703 977 1327 Fragments arranged by size: 3703 2523 1653 1327 977 AgeI A'CCGG_T Cuts at: 9014 9014 Size: 10183 AhdI GACnn_n'nnGTC Cuts at: 8912 8912 Size: 10183 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 2733 3438 4734 1184 Size: 1299 250 705 1296 6633 Fragments arranged by size: 6633 1299 1296 705 250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6129 6161 6129 Size: 32 10151 AlwNI CAG_nnn'CTG Cuts at: 2633 2669 4340 7926 9498 2633 Size: 36 1671 3586 1572 3318 Fragments arranged by size: 3586 3318 1671 1572 36 ApaI G_GGCC'C Cuts at: 3888 5190 6984 3888 Size: 1302 1794 7087 Fragments arranged by size: 7087 1794 1302 ApaLI G'TGCA_C Cuts at: 3939 8240 9396 3939 Size: 4301 1156 4726 Fragments arranged by size: 4726 4301 1156 ApoI r'AATT_y Cuts at: 1493 2759 3020 3212 3597 3678 5087 5198 5672 Size: 1266 261 192 385 81 1409 111 474 Cuts at: 5672 5684 5891 7492 7718 7870 9979 1493 Size: 12 207 1601 226 152 2109 1697 Fragments arranged by size: 2109 1697 1601 1409 1266 474 385 261 226 207 192 152 111 81 12 AseI AT'TA_AT Cuts at: 160 1032 8737 160 Size: 872 7705 1606 Fragments arranged by size: 7705 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10183 AvaI C'yCGr_G Cuts at: 1870 1943 2488 6384 7038 7508 1870 Size: 73 545 3896 654 470 4545 Fragments arranged by size: 4545 3896 654 545 470 73 BamHI G'GATC_C Cuts at: 7513 7880 7513 Size: 367 9816 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 3806 5864 7504 8959 Size: 325 217 627 489 1530 2058 1640 1455 Cuts at: 8959 618 Size: 1842 Fragments arranged by size: 2058 1842 1640 1530 1455 627 489 325 217 BanII G_rGCy'C Cuts at: 1391 3888 4259 5190 6984 7502 1391 Size: 2497 371 931 1794 518 4072 Fragments arranged by size: 4072 2497 1794 931 518 371 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2556 3791 5024 5526 6025 6522 Size: 971 555 102 1235 1233 502 499 497 Cuts at: 6522 7152 928 Size: 630 3959 Fragments arranged by size: 3959 1235 1233 971 630 555 502 499 497 102 BbvCI CC'TCA_GC Cuts at: 2030 2030 Size: 10183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 1998 2021 2032 3890 3924 6050 Size: 34 50 11 23 11 1858 34 2126 Cuts at: 6050 6084 8374 8408 1903 Size: 34 2290 34 3678 Fragments arranged by size: 3678 2290 2126 1858 50 34 34 34 34 23 11 11 BciVI GTATCCnnnnn_n' Cuts at: 2132 4527 8075 9285 2132 Size: 2395 3548 1210 3030 Fragments arranged by size: 3548 3030 2395 1210 BclI T'GATC_A Cuts at: 1548 4350 1548 Size: 2802 7381 BfrBI ATG'CAT Cuts at: 4465 4465 Size: 10183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3363 8794 136 Size: 107 122 71 631 605 1691 5431 1525 Fragments arranged by size: 5431 1691 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4090 6253 6465 10178 4090 Size: 2163 212 3713 4095 Fragments arranged by size: 4095 3713 2163 212 BlpI GC'TnA_GC Cuts at: 5229 5473 7600 5229 Size: 244 2127 7812 Fragments arranged by size: 7812 2127 244 Bme1580I G_kGCm'C Cuts at: 1790 3809 3888 3943 5190 6984 7476 8244 9400 Size: 2019 79 55 1247 1794 492 768 1156 Cuts at: 9400 1790 Size: 2573 Fragments arranged by size: 2573 2019 1794 1247 1156 768 492 79 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2274 4923 6647 Size: 416 53 83 399 24 857 2649 1724 Cuts at: 6647 7914 8872 442 Size: 1267 958 1753 Fragments arranged by size: 2649 1753 1724 1267 958 857 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10183 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3640 7165 3640 Size: 3525 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2177 3057 4218 4296 4432 5199 6248 Size: 876 62 880 1161 78 136 767 1049 Cuts at: 6248 6267 6428 7086 7191 8843 1239 Size: 19 161 658 105 1652 2579 Fragments arranged by size: 2579 1652 1161 1049 880 876 767 658 161 136 105 78 62 19 Bpu10I CC'TnA_GC Cuts at: 2030 3525 4695 6840 7200 2030 Size: 1495 1170 2145 360 5013 Fragments arranged by size: 5013 2145 1495 1170 360 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3245 4310 5540 6074 6923 8308 9173 9471 3245 Size: 1065 1230 534 849 1385 865 298 3957 Fragments arranged by size: 3957 1385 1230 1065 865 849 534 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6707 8846 882 Size: 617 5208 2139 2219 Fragments arranged by size: 5208 2219 2139 617 BsaAI yAC'GTr Cuts at: 493 1204 5084 5417 6722 7974 493 Size: 711 3880 333 1305 1252 2702 Fragments arranged by size: 3880 2702 1305 1252 711 333 BsaBI GATnn'nnATC Cuts at: 4089 7230 10177 4089 Size: 3141 2947 4095 Fragments arranged by size: 4095 3141 2947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6190 7990 8372 9007 Size: 53 83 186 936 4657 1800 382 635 Cuts at: 9007 275 Size: 1451 Fragments arranged by size: 4657 1800 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 8616 9014 9288 9435 9846 4587 Size: 4029 398 274 147 411 4924 Fragments arranged by size: 4924 4029 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3620 3650 5951 5981 6333 Size: 30 165 30 2217 30 2301 30 352 Cuts at: 6333 6363 7084 7114 1178 Size: 30 721 30 4247 Fragments arranged by size: 4247 2301 2217 721 352 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2463 5441 5975 5978 7196 7199 1186 Size: 1277 2978 534 3 1218 3 4170 Fragments arranged by size: 4170 2978 1277 1218 534 3 3 BseYI C'CCAG_C Cuts at: 2221 2699 3118 3498 5127 7135 7331 9386 9882 Size: 478 419 380 1629 2008 196 2055 496 Cuts at: 9882 2221 Size: 2522 Fragments arranged by size: 2522 2055 2008 1629 496 478 419 380 196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 9610 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7633 8394 9422 1540 Size: 275 186 5632 761 1028 2301 Fragments arranged by size: 5632 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2839 3943 4204 4312 8244 8329 9400 2839 Size: 1104 261 108 3932 85 1071 3622 Fragments arranged by size: 3932 3622 1104 1071 261 108 85 BsmI GAATG_Cn' Cuts at: 2903 3832 5460 7694 7787 2903 Size: 929 1628 2234 93 5299 Fragments arranged by size: 5299 2234 1628 929 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7041 7041 Size: 10183 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2839 3809 3888 3943 4204 4259 4312 Size: 399 1049 970 79 55 261 55 53 Cuts at: 4312 5190 6984 7476 7502 8244 8329 9400 1391 Size: 878 1794 492 26 742 85 1071 2174 Fragments arranged by size: 2174 1794 1071 1049 970 878 742 492 399 261 85 79 55 55 53 26 BspEI T'CCGG_A Cuts at: 4587 9846 4587 Size: 5259 4924 BspHI T'CATG_A Cuts at: 8072 9802 8072 Size: 1730 8453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4129 7538 844 Size: 3285 3409 3489 Fragments arranged by size: 3489 3409 3285 BsrBI CCG'CTC Cuts at: 1394 1823 2147 5437 8070 1394 Size: 429 324 3290 2633 3507 Fragments arranged by size: 3507 3290 2633 429 324 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 3241 4875 8678 8852 65 Size: 1183 1323 670 1634 3803 174 1396 Fragments arranged by size: 3803 1634 1396 1323 1183 670 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8827 9014 1700 Size: 110 7017 187 2869 Fragments arranged by size: 7017 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 6917 7258 96 Size: 6821 341 3021 Fragments arranged by size: 6821 3021 341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10183 BssSI C'ACGA_G Cuts at: 8243 9255 8243 Size: 1012 9171 BstAPI GCAn_nnn'nTGC Cuts at: 794 5864 794 Size: 5070 5113 BstBI TT'CG_AA Cuts at: 5930 8987 5930 Size: 3057 7126 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10183 BstXI CCAn_nnnn'nTGG Cuts at: 2826 3035 3880 6636 2826 Size: 209 845 2756 6373 Fragments arranged by size: 6373 2756 845 209 BstYI r'GATC_y Cuts at: 1972 2883 3481 3748 4069 4090 4192 4924 6253 Size: 911 598 267 321 21 102 732 1329 Cuts at: 6253 6465 6484 7513 7880 8265 8282 9734 9820 Size: 212 19 1029 367 385 17 1452 86 Cuts at: 9820 9832 10178 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1329 1029 911 732 598 385 367 346 321 267 212 102 86 21 19 17 12 Bsu36I CC'TnA_GG Cuts at: 2646 3345 4030 4065 5100 2646 Size: 699 685 35 1035 7729 Fragments arranged by size: 7729 1035 699 685 35 BtgI C'CryG_G Cuts at: 513 1065 3752 4526 5206 513 Size: 552 2687 774 680 5490 Fragments arranged by size: 5490 2687 774 680 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3893 487 Size: 683 56 2667 6777 Fragments arranged by size: 6777 2667 683 56 BtsI GCAGTG_nn' Cuts at: 2049 2978 3145 4421 4718 5016 7092 7699 8493 Size: 929 167 1276 297 298 2076 607 794 Cuts at: 8493 8513 2049 Size: 20 3719 Fragments arranged by size: 3719 2076 1276 929 794 607 298 297 167 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10183 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2122 2157 529 Size: 35 1558 35 8555 Fragments arranged by size: 8555 1558 35 35 DraI TTT'AAA Cuts at: 7487 7487 Size: 10183 DraIII CAC_nnn'GTG Cuts at: 4935 6353 4935 Size: 1418 8765 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6602 6819 9190 809 Size: 507 5286 217 2371 1802 Fragments arranged by size: 5286 2371 1802 507 217 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4270 4408 4523 Size: 54 1425 50 275 18 2440 138 115 Cuts at: 4523 6329 6638 7630 8519 10148 8 Size: 1806 309 992 889 1629 43 Fragments arranged by size: 2440 1806 1629 1425 992 889 309 275 138 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7630 1537 Size: 275 5818 4090 Fragments arranged by size: 5818 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 3609 3665 4505 4586 4677 5100 5577 Size: 1518 738 56 840 81 91 423 477 Cuts at: 5577 5709 5946 6384 7239 8113 1353 Size: 132 237 438 855 874 3423 Fragments arranged by size: 3423 1518 874 855 840 738 477 438 423 237 132 91 81 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2845 5749 8770 9144 9290 387 Size: 1064 138 1256 2904 3021 374 146 1280 Fragments arranged by size: 3021 2904 1280 1256 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2177 3057 3686 4218 4296 4432 Size: 393 483 62 880 629 532 78 136 Cuts at: 4432 4775 5199 5358 6248 6267 6428 7086 7191 Size: 343 424 159 890 19 161 658 105 Cuts at: 7191 7290 7326 7427 7437 8246 8843 9630 9898 Size: 99 36 101 10 809 597 787 268 Cuts at: 9898 1239 Size: 1524 Fragments arranged by size: 1524 890 880 809 787 658 629 597 532 483 424 393 343 268 161 159 136 105 101 99 78 62 36 19 10 EcoNI CCTnn'n_nnAGG Cuts at: 7286 7286 Size: 10183 EcoO109I rG'GnC_Cy Cuts at: 2642 3884 3956 4869 5186 5795 6980 6981 2642 Size: 1242 72 913 317 609 1185 1 5844 Fragments arranged by size: 5844 1242 1185 913 609 317 72 1 EcoRI G'AATT_C Cuts at: 3678 7492 7870 3678 Size: 3814 378 5991 Fragments arranged by size: 5991 3814 378 EcoRV GAT'ATC Cuts at: 3322 3322 Size: 10183 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2212 2212 Size: 10183 FspI TGC'GCA Cuts at: 4058 5150 5648 8689 4058 Size: 1092 498 3041 5552 Fragments arranged by size: 5552 3041 1092 498 FspAI rTGC'GCAy Cuts at: 4058 5150 5648 4058 Size: 1092 498 8593 Fragments arranged by size: 8593 1092 498 HaeII r_GCGC'y Cuts at: 1678 9330 1678 Size: 7652 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2458 2490 2538 2570 2615 Size: 32 135 32 886 32 48 32 45 Cuts at: 2615 2629 2647 2661 3328 3360 4504 4536 5294 Size: 14 18 14 667 32 1144 32 758 Cuts at: 5294 5315 5326 5347 5490 5522 5538 5570 6682 Size: 21 11 21 143 32 16 32 1112 Cuts at: 6682 6714 8824 8856 8898 8930 1373 Size: 32 2110 32 42 32 2626 Fragments arranged by size: 2626 2110 1144 1112 886 758 667 143 135 48 45 42 32 32 32 32 32 32 32 32 32 32 32 21 21 18 16 14 14 11 HincII GTy'rAC Cuts at: 669 1657 1938 5160 7527 7773 669 Size: 988 281 3222 2367 246 3079 Fragments arranged by size: 3222 3079 2367 988 281 246 HindIII A'AGCT_T Cuts at: 748 5786 748 Size: 5038 5145 HpaI GTT'AAC Cuts at: 7773 7773 Size: 10183 KpnI G_GTAC'C Cuts at: 7508 7508 Size: 10183 MfeI C'AATT_G Cuts at: 7782 7782 Size: 10183 MluI A'CGCG_T Cuts at: 10059 10059 Size: 10183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3525 3994 5949 6115 9297 9481 1305 Size: 2220 469 1955 166 3182 184 2007 Fragments arranged by size: 3182 2220 2007 1955 469 184 166 MscI TGG'CCA Cuts at: 10 64 1489 1832 4272 4410 6640 10 Size: 54 1425 343 2440 138 2230 3553 Fragments arranged by size: 3553 2440 2230 1425 343 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3878 4628 5419 5499 7467 8141 Size: 648 1833 879 750 791 80 1968 674 Cuts at: 8141 8500 8659 518 Size: 359 159 2042 Fragments arranged by size: 2042 1968 1833 879 791 750 674 648 359 159 80 MspA1I CmG'CkG Cuts at: 1606 2155 3283 5127 5336 5410 5627 7375 7599 Size: 549 1128 1844 209 74 217 1748 224 Cuts at: 7599 8276 9424 9669 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1844 1748 1148 1128 677 549 245 224 217 209 74 NcoI C'CATG_G Cuts at: 513 1065 3752 513 Size: 552 2687 6944 Fragments arranged by size: 6944 2687 552 NdeI CA'TA_TG Cuts at: 387 5877 387 Size: 5490 4693 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10183 NotI GC'GGCC_GC Cuts at: 7630 7630 Size: 10183 NsiI A_TGCA'T Cuts at: 4467 4467 Size: 10183 NspI r_CATG'y Cuts at: 1132 1229 3730 5383 5862 7451 7541 9086 1132 Size: 97 2501 1653 479 1589 90 1545 2229 Fragments arranged by size: 2501 2229 1653 1589 1545 479 97 90 PasI CC'CwG_GG Cuts at: 1348 6977 1348 Size: 5629 4554 PciI A'CATG_T Cuts at: 3726 5379 9082 3726 Size: 1653 3703 4827 Fragments arranged by size: 4827 3703 1653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2699 2893 1108 Size: 1591 194 8398 Fragments arranged by size: 8398 1591 194 PfoI T'CCnGG_A Cuts at: 2886 4195 6225 2886 Size: 1309 2030 6844 Fragments arranged by size: 6844 2030 1309 PmeI GTTT'AAAC Cuts at: 7487 7487 Size: 10183 PmlI CAC'GTG Cuts at: 5417 5417 Size: 10183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8252 8284 9765 9797 8252 Size: 32 1481 32 8638 Fragments arranged by size: 8638 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2642 3956 5795 2642 Size: 1314 1839 7030 Fragments arranged by size: 7030 1839 1314 PshAI GACnn'nnGTC Cuts at: 1613 5046 1613 Size: 3433 6750 PsiI TTA'TAA Cuts at: 4173 7753 4173 Size: 3580 6603 PspOMI G'GGCC_C Cuts at: 3884 5186 6980 3884 Size: 1302 1794 7087 Fragments arranged by size: 7087 1794 1302 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6845 6877 6845 Size: 32 10151 PstI C_TGCA'G Cuts at: 830 4284 7535 830 Size: 3454 3251 3478 Fragments arranged by size: 3478 3454 3251 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10183 PvuII CAG'CTG Cuts at: 2155 3283 5336 5410 5627 7375 2155 Size: 1128 2053 74 217 1748 4963 Fragments arranged by size: 4963 2053 1748 1128 217 74 SalI G'TCGA_C Cuts at: 1936 7525 1936 Size: 5589 4594 SapI GCTCTTCn'nnn_ Cuts at: 2871 5946 2871 Size: 3075 7108 SbfI CC_TGCA'GG Cuts at: 7535 7535 Size: 10183 ScaI AGT'ACT Cuts at: 3929 8431 10143 3929 Size: 4502 1712 3969 Fragments arranged by size: 4502 3969 1712 SfcI C'TryA_G Cuts at: 826 1045 2322 4280 4481 5276 7531 8666 9347 Size: 219 1277 1958 201 795 2255 1135 681 Cuts at: 9347 9538 826 Size: 191 1471 Fragments arranged by size: 2255 1958 1471 1277 1135 795 681 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10183 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10183 SmaI CCC'GGG Cuts at: 1872 2490 7510 1872 Size: 618 5020 4545 Fragments arranged by size: 5020 4545 618 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 4289 4652 5528 5555 5762 Size: 197 926 1281 1065 363 876 27 207 Cuts at: 5762 5789 6089 6902 7038 8287 9188 9450 820 Size: 27 300 813 136 1249 901 262 1553 Fragments arranged by size: 1553 1281 1249 1065 926 901 876 813 363 300 262 207 197 136 27 27 SnaBI TAC'GTA Cuts at: 493 5084 6722 7974 493 Size: 4591 1638 1252 2702 Fragments arranged by size: 4591 2702 1638 1252 SpeI A'CTAG_T Cuts at: 152 152 Size: 10183 SphI G_CATG'C Cuts at: 1132 7451 7541 1132 Size: 6319 90 3774 Fragments arranged by size: 6319 3774 90 SspI AAT'ATT Cuts at: 5 52 3823 8107 5 Size: 47 3771 4284 2081 Fragments arranged by size: 4284 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10183 StyI C'CwwG_G Cuts at: 513 1065 2280 3449 3639 3752 4853 7126 7622 Size: 552 1215 1169 190 113 1101 2273 496 Cuts at: 7622 513 Size: 3074 Fragments arranged by size: 3074 2273 1215 1169 1101 552 496 190 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3110 3442 3890 4636 6623 6666 7127 Size: 1255 1530 332 448 746 1987 43 461 Cuts at: 7127 8210 8227 8380 8565 9010 325 Size: 1083 17 153 185 445 1498 Fragments arranged by size: 1987 1530 1498 1255 1083 746 461 448 445 332 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2309 3927 6825 Size: 274 80 33 51 1443 332 1618 2898 Cuts at: 6825 6917 7258 8429 10141 96 Size: 92 341 1171 1712 138 Fragments arranged by size: 2898 1712 1618 1443 1171 341 332 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 2260 2292 6258 6290 7084 7116 10143 Size: 32 60 32 3966 32 794 32 3027 Cuts at: 10143 10175 2168 Size: 32 2176 Fragments arranged by size: 3966 3027 2176 794 60 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5550 6214 1316 Size: 4234 664 5285 Fragments arranged by size: 5285 4234 664 XbaI T'CTAG_A Cuts at: 3197 6766 7519 3197 Size: 3569 753 5861 Fragments arranged by size: 5861 3569 753 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3070 3070 Size: 10183 XhoI C'TCGA_G Cuts at: 1943 7038 1943 Size: 5095 5088 XmaI C'CCGG_G Cuts at: 1870 2488 7508 1870 Size: 618 5020 4545 Fragments arranged by size: 5020 4545 618 XmnI GAAnn'nnTTC Cuts at: 2751 8312 2751 Size: 5561 4622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7991 276 Size: 53 83 186 936 6457 2468 Fragments arranged by size: 6457 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoICRI FseI KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI