(Linear) (Six-base) MAPSORT of: KIEE3488.seq Check: 7087 from: 1 to: 5478 KIEE3488.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AccI GT'mk_AC Cuts at: 0 320 5478 Size: 320 5158 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1681 2770 3353 5285 5321 5422 5432 5478 Size: 1681 1089 583 1932 36 101 10 46 Fragments arranged by size: 1932 1681 1089 583 101 46 36 10 AflII C'TTAA_G Cuts at: 0 2647 3523 3757 3784 5478 Size: 2647 876 234 27 1694 Fragments arranged by size: 2647 1694 876 234 27 AflIII A'CryG_T Cuts at: 0 1721 3374 5478 Size: 1721 1653 2104 Fragments arranged by size: 2104 1721 1653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 478 728 1433 2729 5478 Size: 478 250 705 1296 2749 Fragments arranged by size: 2749 1296 705 478 250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4124 4156 5478 Size: 4124 32 1322 Fragments arranged by size: 4124 1322 32 AlwNI CAG_nnn'CTG Cuts at: 0 628 664 2335 5478 Size: 628 36 1671 3143 Fragments arranged by size: 3143 1671 628 36 ApaI G_GGCC'C Cuts at: 0 1883 3185 4979 5478 Size: 1883 1302 1794 499 Fragments arranged by size: 1883 1794 1302 499 ApaLI G'TGCA_C Cuts at: 0 1934 5478 Size: 1934 3544 ApoI r'AATT_y Cuts at: 0 754 1015 1207 1592 1673 3082 3193 3667 Size: 754 261 192 385 81 1409 111 474 Cuts at: 3667 3679 3886 5478 Size: 12 207 1592 Fragments arranged by size: 1592 1409 754 474 385 261 207 192 111 81 12 AvaI C'yCGr_G Cuts at: 0 483 4379 5033 5478 Size: 483 3896 654 445 Fragments arranged by size: 3896 654 483 445 BanI G'GyrC_C Cuts at: 0 271 1801 3859 5478 Size: 271 1530 2058 1619 Fragments arranged by size: 2058 1619 1530 271 BanII G_rGCy'C Cuts at: 0 1883 2254 3185 4979 5478 Size: 1883 371 931 1794 499 Fragments arranged by size: 1883 1794 931 499 371 BbsI GAAGACnn'nnnn_ Cuts at: 0 449 551 1786 3019 3521 4020 4517 5147 Size: 449 102 1235 1233 502 499 497 630 Cuts at: 5147 5478 Size: 331 Fragments arranged by size: 1235 1233 630 502 499 497 449 331 102 BbvCI CC'TCA_GC Cuts at: 0 25 5478 Size: 25 5453 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 27 1885 1919 4045 4079 5478 Size: 27 1858 34 2126 34 1399 Fragments arranged by size: 2126 1858 1399 34 34 27 BciVI GTATCCnnnnn_n' Cuts at: 0 127 2522 5478 Size: 127 2395 2956 Fragments arranged by size: 2956 2395 127 BclI T'GATC_A Cuts at: 0 2345 5478 Size: 2345 3133 BfrBI ATG'CAT Cuts at: 0 2460 5478 Size: 2460 3018 BglI GCCn_nnn'nGGC Cuts at: 0 1358 5478 Size: 1358 4120 BglII A'GATC_T Cuts at: 0 2085 4248 4460 5478 Size: 2085 2163 212 1018 Fragments arranged by size: 2163 2085 1018 212 BlpI GC'TnA_GC Cuts at: 0 3224 3468 5478 Size: 3224 244 2010 Fragments arranged by size: 3224 2010 244 Bme1580I G_kGCm'C Cuts at: 0 1804 1883 1938 3185 4979 5471 5478 Size: 1804 79 55 1247 1794 492 7 Fragments arranged by size: 1804 1794 1247 492 79 55 7 BmrI ACTGGGnnnn_n' Cuts at: 0 269 2918 4642 5478 Size: 269 2649 1724 836 Fragments arranged by size: 2649 1724 836 269 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1635 5160 5478 Size: 1635 3525 318 Fragments arranged by size: 3525 1635 318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 172 1052 2213 2291 2427 3194 4243 Size: 110 62 880 1161 78 136 767 1049 Cuts at: 4243 4262 4423 5081 5186 5478 Size: 19 161 658 105 292 Fragments arranged by size: 1161 1049 880 767 658 292 161 136 110 105 78 62 19 Bpu10I CC'TnA_GC Cuts at: 0 25 1520 2690 4835 5195 5478 Size: 25 1495 1170 2145 360 283 Fragments arranged by size: 2145 1495 1170 360 283 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1240 2305 3535 4069 4918 5478 Size: 1240 1065 1230 534 849 560 Fragments arranged by size: 1240 1230 1065 849 560 534 BsaI GGTCTCn'nnnn_ Cuts at: 0 4702 5478 Size: 4702 776 BsaAI yAC'GTr Cuts at: 0 3079 3412 4717 5478 Size: 3079 333 1305 761 Fragments arranged by size: 3079 1305 761 333 BsaBI GATnn'nnATC Cuts at: 0 2084 5225 5478 Size: 2084 3141 253 Fragments arranged by size: 3141 2084 253 BsaHI Gr'CG_yC Cuts at: 0 4185 5478 Size: 4185 1293 BsaWI w'CCGG_w Cuts at: 0 2582 5478 Size: 2582 2896 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1615 1645 3946 3976 4328 4358 5079 5109 Size: 1615 30 2301 30 352 30 721 30 Cuts at: 5109 5478 Size: 369 Fragments arranged by size: 2301 1615 721 369 352 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 458 3436 3970 3973 5191 5194 5478 Size: 458 2978 534 3 1218 3 284 Fragments arranged by size: 2978 1218 534 458 284 3 3 BseYI C'CCAG_C Cuts at: 0 216 694 1113 1493 3122 5130 5326 5478 Size: 216 478 419 380 1629 2008 196 152 Fragments arranged by size: 2008 1629 478 419 380 216 196 152 BsiHKAI G_wGCw'C Cuts at: 0 834 1938 2199 2307 5478 Size: 834 1104 261 108 3171 Fragments arranged by size: 3171 1104 834 261 108 BsmI GAATG_Cn' Cuts at: 0 898 1827 3455 5478 Size: 898 929 1628 2023 Fragments arranged by size: 2023 1628 929 898 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5036 5478 Size: 5036 442 Bsp1286I G_dGCh'C Cuts at: 0 834 1804 1883 1938 2199 2254 2307 3185 Size: 834 970 79 55 261 55 53 878 Cuts at: 3185 4979 5471 5478 Size: 1794 492 7 Fragments arranged by size: 1794 970 878 834 492 261 79 55 55 53 7 BspEI T'CCGG_A Cuts at: 0 2582 5478 Size: 2582 2896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2124 5478 Size: 2124 3354 BsrBI CCG'CTC Cuts at: 0 142 3432 5478 Size: 142 3290 2046 Fragments arranged by size: 3290 2046 142 BsrDI GCAATG_nn' Cuts at: 0 566 1236 2870 5478 Size: 566 670 1634 2608 Fragments arranged by size: 2608 1634 670 566 BsrGI T'GTAC_A Cuts at: 0 4912 5253 5478 Size: 4912 341 225 Fragments arranged by size: 4912 341 225 BstAPI GCAn_nnn'nTGC Cuts at: 0 3859 5478 Size: 3859 1619 BstBI TT'CG_AA Cuts at: 0 3925 5478 Size: 3925 1553 BstXI CCAn_nnnn'nTGG Cuts at: 0 821 1030 1875 4631 5478 Size: 821 209 845 2756 847 Fragments arranged by size: 2756 847 845 821 209 BstYI r'GATC_y Cuts at: 0 878 1476 1743 2064 2085 2187 2919 4248 Size: 878 598 267 321 21 102 732 1329 Cuts at: 4248 4460 4479 5478 Size: 212 19 999 Fragments arranged by size: 1329 999 878 732 598 321 267 212 102 21 19 Bsu36I CC'TnA_GG Cuts at: 0 641 1340 2025 2060 3095 5478 Size: 641 699 685 35 1035 2383 Fragments arranged by size: 2383 1035 699 685 641 35 BtgI C'CryG_G Cuts at: 0 1747 2521 3201 5478 Size: 1747 774 680 2277 Fragments arranged by size: 2277 1747 774 680 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1888 5478 Size: 1888 3590 BtsI GCAGTG_nn' Cuts at: 0 44 973 1140 2416 2713 3011 5087 5478 Size: 44 929 167 1276 297 298 2076 391 Fragments arranged by size: 2076 1276 929 391 298 297 167 44 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 117 152 5478 Size: 117 35 5326 Fragments arranged by size: 5326 117 35 DraIII CAC_nnn'GTG Cuts at: 0 2930 4348 5478 Size: 2930 1418 1130 Fragments arranged by size: 2930 1418 1130 DrdI GACnn_nn'nnGTC Cuts at: 0 4597 4814 5478 Size: 4597 217 664 Fragments arranged by size: 4597 664 217 EaeI y'GGCC_r Cuts at: 0 2265 2403 2518 4324 4633 5478 Size: 2265 138 115 1806 309 845 Fragments arranged by size: 2265 1806 845 309 138 115 EarI CTCTTCn'nnn_ Cuts at: 0 866 1604 1660 2500 2581 2672 3095 3572 Size: 866 738 56 840 81 91 423 477 Cuts at: 3572 3704 3941 4379 5234 5478 Size: 132 237 438 855 244 Fragments arranged by size: 866 855 840 738 477 438 423 244 237 132 91 81 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 840 3744 5478 Size: 840 2904 1734 Fragments arranged by size: 2904 1734 840 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 172 1052 1681 2213 2291 2427 2770 Size: 110 62 880 629 532 78 136 343 Cuts at: 2770 3194 3353 4243 4262 4423 5081 5186 5285 Size: 424 159 890 19 161 658 105 99 Cuts at: 5285 5321 5422 5432 5478 Size: 36 101 10 46 Fragments arranged by size: 890 880 658 629 532 424 343 161 159 136 110 105 101 99 78 62 46 36 19 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 5281 5478 Size: 5281 197 EcoO109I rG'GnC_Cy Cuts at: 0 637 1879 1951 2864 3181 3790 4975 4976 Size: 637 1242 72 913 317 609 1185 1 Cuts at: 4976 5478 Size: 502 Fragments arranged by size: 1242 1185 913 637 609 502 317 72 1 EcoRI G'AATT_C Cuts at: 0 1673 5478 Size: 1673 3805 EcoRV GAT'ATC Cuts at: 0 1317 5478 Size: 1317 4161 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 207 5478 Size: 207 5271 FspI TGC'GCA Cuts at: 0 2053 3145 3643 5478 Size: 2053 1092 498 1835 Fragments arranged by size: 2053 1835 1092 498 FspAI rTGC'GCAy Cuts at: 0 2053 3145 3643 5478 Size: 2053 1092 498 1835 Fragments arranged by size: 2053 1835 1092 498 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 453 485 533 565 610 624 642 656 Size: 453 32 48 32 45 14 18 14 Cuts at: 656 1323 1355 2499 2531 3289 3310 3321 3342 Size: 667 32 1144 32 758 21 11 21 Cuts at: 3342 3485 3517 3533 3565 4677 4709 5478 Size: 143 32 16 32 1112 32 769 Fragments arranged by size: 1144 1112 769 758 667 453 143 48 45 32 32 32 32 32 32 32 21 21 18 16 14 14 11 HincII GTy'rAC Cuts at: 0 3155 5478 Size: 3155 2323 HindIII A'AGCT_T Cuts at: 0 3781 5478 Size: 3781 1697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1520 1989 3944 4110 5478 Size: 1520 469 1955 166 1368 Fragments arranged by size: 1955 1520 1368 469 166 MscI TGG'CCA Cuts at: 0 2267 2405 4635 5478 Size: 2267 138 2230 843 Fragments arranged by size: 2267 2230 843 138 MslI CAynn'nnrTG Cuts at: 0 994 1873 2623 3414 3494 5462 5478 Size: 994 879 750 791 80 1968 16 Fragments arranged by size: 1968 994 879 791 750 80 16 MspA1I CmG'CkG Cuts at: 0 150 1278 3122 3331 3405 3622 5370 5478 Size: 150 1128 1844 209 74 217 1748 108 Fragments arranged by size: 1844 1748 1128 217 209 150 108 74 NcoI C'CATG_G Cuts at: 0 1747 5478 Size: 1747 3731 NdeI CA'TA_TG Cuts at: 0 3872 5478 Size: 3872 1606 NsiI A_TGCA'T Cuts at: 0 2462 5478 Size: 2462 3016 NspI r_CATG'y Cuts at: 0 1725 3378 3857 5446 5478 Size: 1725 1653 479 1589 32 Fragments arranged by size: 1725 1653 1589 479 32 PasI CC'CwG_GG Cuts at: 0 4972 5478 Size: 4972 506 PciI A'CATG_T Cuts at: 0 1721 3374 5478 Size: 1721 1653 2104 Fragments arranged by size: 2104 1721 1653 PflMI CCAn_nnn'nTGG Cuts at: 0 694 888 5478 Size: 694 194 4590 Fragments arranged by size: 4590 694 194 PfoI T'CCnGG_A Cuts at: 0 881 2190 4220 5478 Size: 881 1309 2030 1258 Fragments arranged by size: 2030 1309 1258 881 PmlI CAC'GTG Cuts at: 0 3412 5478 Size: 3412 2066 PpuMI rG'GwC_Cy Cuts at: 0 637 1951 3790 5478 Size: 637 1314 1839 1688 Fragments arranged by size: 1839 1688 1314 637 PshAI GACnn'nnGTC Cuts at: 0 3041 5478 Size: 3041 2437 PsiI TTA'TAA Cuts at: 0 2168 5478 Size: 2168 3310 PspOMI G'GGCC_C Cuts at: 0 1879 3181 4975 5478 Size: 1879 1302 1794 503 Fragments arranged by size: 1879 1794 1302 503 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 4840 4872 5478 Size: 4840 32 606 Fragments arranged by size: 4840 606 32 PstI C_TGCA'G Cuts at: 0 2279 5478 Size: 2279 3199 PvuII CAG'CTG Cuts at: 0 150 1278 3331 3405 3622 5370 5478 Size: 150 1128 2053 74 217 1748 108 Fragments arranged by size: 2053 1748 1128 217 150 108 74 SapI GCTCTTCn'nnn_ Cuts at: 0 866 3941 5478 Size: 866 3075 1537 Fragments arranged by size: 3075 1537 866 ScaI AGT'ACT Cuts at: 0 1924 5478 Size: 1924 3554 SfcI C'TryA_G Cuts at: 0 317 2275 2476 3271 5478 Size: 317 1958 201 795 2207 Fragments arranged by size: 2207 1958 795 317 201 SmaI CCC'GGG Cuts at: 0 485 5478 Size: 485 4993 SmlI C'TyrA_G Cuts at: 0 1219 2284 2647 3523 3550 3757 3784 4084 Size: 1219 1065 363 876 27 207 27 300 Cuts at: 4084 4897 5033 5478 Size: 813 136 445 Fragments arranged by size: 1219 1065 876 813 445 363 300 207 136 27 27 SnaBI TAC'GTA Cuts at: 0 3079 4717 5478 Size: 3079 1638 761 Fragments arranged by size: 3079 1638 761 SphI G_CATG'C Cuts at: 0 5446 5478 Size: 5446 32 SspI AAT'ATT Cuts at: 0 1818 5478 Size: 1818 3660 StyI C'CwwG_G Cuts at: 0 275 1444 1634 1747 2848 5121 5478 Size: 275 1169 190 113 1101 2273 357 Fragments arranged by size: 2273 1169 1101 357 275 190 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1105 1437 1885 2631 4618 4661 5122 5478 Size: 1105 332 448 746 1987 43 461 356 Fragments arranged by size: 1987 1105 746 461 448 356 332 43 TatI w'GTAC_w Cuts at: 0 304 1922 4820 4912 5253 5478 Size: 304 1618 2898 92 341 225 Fragments arranged by size: 2898 1618 341 304 225 92 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 163 195 255 287 4253 4285 5079 5111 Size: 163 32 60 32 3966 32 794 32 Cuts at: 5111 5478 Size: 367 Fragments arranged by size: 3966 794 367 163 60 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3545 4209 5478 Size: 3545 664 1269 Fragments arranged by size: 3545 1269 664 XbaI T'CTAG_A Cuts at: 0 1192 4761 5478 Size: 1192 3569 717 Fragments arranged by size: 3569 1192 717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1065 5478 Size: 1065 4413 XhoI C'TCGA_G Cuts at: 0 5033 5478 Size: 5033 445 XmaI C'CCGG_G Cuts at: 0 483 5478 Size: 483 4995 XmnI GAAnn'nnTTC Cuts at: 0 746 5478 Size: 746 4732 Enzymes that do cut: AccI AcuI AflII AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SapI ScaI SfcI SmaI SmlI SnaBI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI BbeI BmgBI BmtI BsgI BsiEI BsiWI BspHI BsrFI BssHII BssSI BstEII BstZ17I ClaI DraI EagI EcoICRI FseI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PmeI PpiI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SpeI SrfI StuI SwaI ZraI